A5015799757- Medical Imaging Techniques and Applications
- Advanced X-ray and CT Imaging
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Photoacoustic and Ultrasonic Imaging
- Glioma Diagnosis and Treatment
- Nerve injury and regeneration
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Neuroscience and Neural Engineering
- Spinal Cord Injury Research
- Multiple Sclerosis Research Studies
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Neurogenesis and neuroplasticity mechanisms
- Spectroscopy Techniques in Biomedical and Chemical Research
- Radiomics and Machine Learning in Medical Imaging
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Neuroscience and Neuropharmacology Research
- Radiation Dose and Imaging
- Olfactory and Sensory Function Studies
- Nuclear Physics and Applications
- Bee Products Chemical Analysis
- Advanced MRI Techniques and Applications
Centre National de la Recherche Scientifique
2010-2024
Aix-Marseille Université
2014-2024
Institut de Neurosciences de la Timone
2014-2024
Institut Universitaire de France
2021-2024
Institut Fresnel
2017
Centrale Marseille
2017
Institut de Biologie du Développement Marseille
2006-2016
Centre de physique des particules de Marseille
2009-2012
RIKEN Center for Brain Science
2004
Centre de Gestion Scientifique
2003
Understanding the endogenous repair capacity of spinal cord is pivotal to develop strategies improve it. Here we design a paradigm lesion in dorsal column using 2-photon microscopy technique dynamically and chronically monitor simultaneous changes vascular axonal networks living mice up 4 months postinjury. High-resolution images showed that early explorative sprouting surviving injured axons resulted extensive regrowth until past site within 2 months. Blood vessel density was transiently...
Repeated in vivo two-photon imaging of adult mammalian spinal cords, with subcellular resolution, would be crucial for understanding cellular mechanisms under normal and pathological conditions. Current methods are limited because they require surgery each session. Here we report a simple glass window methodology avoiding repeated surgical procedures subsequent inflammation. We applied this strategy to follow axon integrity the inflammatory response over months by multicolour transgenic...
Debarbieux, Franck, Jennifer Brunton, and Serge Charpak. Effect of bicuculline on thalamic activity: a direct blockade I AHP in reticularis neurons. J. Neurophysiol. 79: 2911–2918, 1998. The reticular nucleus (RTN) is the major source inhibitory contacts thalamus thus plays an important role regulating excitability thalamocortical network. Inhibition occurs through GABAergic synapses relay cells as well between neurons themselves. Here we report that mechanisms this inhibition, which...
Drug delivery to the central nervous system is hindered by presence of physiological barriers such as blood-brain barrier. To accomplish task nutrient transport, brain endothelium endowed with various transport systems, including receptor-mediated transcytosis (RMT). This can be used shuttle therapeutics into (CNS) in a noninvasive manner. Therefore, low-density lipoprotein receptor (LDLR) relevant target for delivering drugs. From an initial phage display biopanning, series peptide ligands...
Abstract In both multiple sclerosis and its model experimental autoimmune encephalomyelitis (EAE), the extent of resident microglia activation infiltration monocyte-derived cells to CNS is positively correlated tissue damage. To address phenotype characterization different cell subsets, their spatio-temporal distributions contributions disease development we induced EAE in Thy1-CFP//LysM-EGFP//CD11c-EYFP reporter mice. We combined high content flow cytometry, immunofluorescence two-photon...
The majority of intravital studies on brain tumor in living animal so far rely dual color imaging. We describe here a multiphoton imaging protocol to dynamically characterize the interactions between six cellular components mouse. applied this methodology clinically relevant glioblastoma multiforme (GBM) model designed reporter mice with targeted cell populations labeled by fluorescent proteins different colors. This permitted us make non-invasive longitudinal and multi-scale observations...
After spinal cord injury (SCI), resident and peripheral myelomonocytic cells are recruited to the site play a role in progression. These important for clearing cellular debris, can modulate retraction growth of axons vitro. However, their precise spatiotemporal recruitment dynamics is unknown, respective roles after SCI remain heavily debated. Using chronic, quantitative intravital two-photon microscopy adult mice with SCI, here we show that infiltrating lysozyme M (LysM(+)) CD11c(+) have...
Inflammatory cells, an integral component of tumor evolution, are present in Glioblastomas multiforme (GBM). To address the cellular basis and dynamics inflammatory microenvironment GBM, we established orthotopic syngenic model by grafting GL261-DsRed cells immunocompetent transgenic LysM-EGFP//CD11c-EYFP reporter mice. We combined dynamic spectral two-photon imaging with multiparametric cytometry multicolor immunostaining to characterize spatio-temporal distribution, morphology activity...
We previously reported that embryonic motor cortical neurons transplanted immediately after lesions in the adult mouse cortex restored damaged pathways. A critical barrier hindering application of transplantation strategies for a wide range traumatic injuries is determination suitable time window therapeutic intervention. Here, we report 1 week delay between lesion and significantly enhances graft vascularization, survival, proliferation grafted cells. More importantly, dramatically...
Odors evoke beta-gamma frequency field potential oscillations in the olfactory systems of awake and anesthetized vertebrates. In rat bulb, these reflect synchronous discharges mitral cells that result from both their intrinsic membrane properties dendrodendritic interactions with local inhibitory interneurons. Activation synapses is purportedly involved odor memory contrast enhancement. Here we investigate vivo to what extent action potentials propagate remote sites entire dendritic tree if...
XVAD3 is a single photon counting chip based on hybrid pixel counters, with low noise, high dynamics and speed readout. Its features have been improved to provide an rate capability, very threshold, energy discrimination fast image The designed in 0.25 mum IBM technology, contains 9600 pixels (130 times 130 mum) distributed into 80 columns of 120 elements each. An innovative architecture has order prevent the digital circuits from disturbing sensitive analogue cells. XPAD3 was realized two...
Experimental autoimmune encephalomyelitis (EAE) in adult rodents is the standard experimental model for studying autonomic demyelinating diseases such as multiple sclerosis. Here we present a low-cost and reproducible glass window implantation protocol that suitable intravital microscopy dynamics of spinal cord cytoarchitecture with subcellular resolution live mice EAE. Briefly, surgically expose vertebrae T12-L2 construct chamber around exposed using combination cyanoacrylate dental cement....
Abstract Tumor-associated macrophages/microglia (TAMs) are highly plastic and heterogeneous immune cells that can be immune-supportive or tumor-supportive depending of the microenvironment. TAMs most abundant in glioblastoma (GB), play a key role immunosuppression. Therefore, reprogramming toward is promising strategy to overcome By leveraging scRNAseq human GB databases, we identified within tumor microenvironment, ML-IAP, member Inhibitor Apoptosis Proteins (IAP) known regulate...
The spatiotemporal and longitudinal monitoring of cellular processes occurring in tumors is critical for oncological research. We focused on glioblastoma multiforme (GBM), an untreatable highly vascularized brain tumor whose progression thought to critically depend the oxygen metabolites supplied by blood vessels. optimized protocols orthotopic GBM grafting mice that were able recapitulate biophysical constraints normally governing suitable intravital multiphoton microscopy. repeatedly...
Glioblastoma multiforme (GBM) is the most aggressive form of brain tumors with no curative treatments available to date. Murine models this pathology rely on injection a suspension glioma cells into parenchyma following incision dura-mater. Whereas have be injected superficially accessible intravital two-photon microscopy, superficial injections fail recapitulate physiopathological conditions. Indeed, escaping through tract tumor reach extra-dural space where they expand abnormally fast in...
We investigate the capability to perform K-edge imaging with newly developed micro-CT PIXSCAN based on XPAD3 hybrid pixel detector. The detector surface of <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$8~{\rm cm} \times 11~{\rm cm}$</tex></formula> makes it possible whole body mouse imaging. present a proof principle mouse-size phantoms filled Silver and Iodine solutions. Results are compared...
Demyelination and axon degeneration are major events in all neurodegenerative diseases, including multiple sclerosis. Intoxication of oligodendrocytes with lysophosphatidylcholine (LPC) is often used as a selective model focal reversible demyelination thought to have no incidence for neurons. To characterize the cascade cellular involved LPC-induced demyelination, we combined intravital coherent antistoke Raman scattering microscopy two-photon fluorescence multicolor transgenic reporter...
The extension of the lesion following spinal cord injury (SCI) poses a major challenge for regenerating axons, which must grow across several centimetres damaged tissue in absence ordered guidance cues. Biofunctionalized electroconducting microfibres (MFs) that provide biochemical signals, as well electrical and mechanical cues, offer promising therapeutic approach to help axons overcome this blind journey. We used poly(3,4-ethylenedioxythiophene)-coated carbon MFs functionalized with cell...
Synapses formed by the olfactory nerve (ON) provide source of excitatory synaptic input onto mitral cells (MC) in bulb. These synapses, which relay odor-specific inputs, are confined to distally tufted single primary dendrites MCs, first stage central processing. β-adrenergic modulation electrical and chemical signaling at these synapses may be involved early odor preference learning. To investigate this possibility, we combined electrophysiological recordings with calcium imaging bulb...
The aims of the pixel detector CT-scanner for small animal imaging PIXSCAN are to provide high count rate and fast image readout, improve soft tissue contrast by energy discrimination, dynamic range low noise. As a proof principle, photon counting built X-ray synchrotron radiations was used build prototype demonstrator device. This obtain first images mice reconstructed FDK algorithm. A new chip with 130 /spl times/ mu/m/sup 2/ pixels (XPAD3) in sub-micron technology has been designed,...