Recent genetic data on schizophrenia (SCZ) have suggested that proteins of the postsynaptic density excitatory synapses a role in its etiology. Mutations three SHANK genes encoding for scaffolding been shown to represent risk factors autism spectrum disorders and other neurodevelopmental disorders. To address if SHANK2 variants are associated with SCZ, we sequenced 481 patients 659 unaffected individuals. We identified significant increase number rare (minor allele frequency<1%) missense SCZ...

Linezolid is an oxazolidinone antibiotic and considered a last-resort treatment option for serious infections caused by problematic Gram-positive pathogens, including vancomycin-resistant enterococci. The present study aimed to explore the linezolid resistance mechanisms genomic characteristics of two vancomycin-susceptible Enterococcus faecalis isolates from Bulgaria. strains designated Efs2503-bg (inpatient Pleven) Efs966-bg (outpatient Varna) were recovered wounds in 2018 2023,...

As belonging to one of the most isolated continents on our planet, microbial composition different environments in Antarctica could hold a plethora undiscovered species with potential for biotechnological applications. This manuscript delineates discoveries after an expedition Bulgarian Antarctic Base &amp;quot;St. Kliment Ohridski&amp;quot; situated Livingston Island, Antarctica. Amplicon-based metagenomics targeting 16S rRNA genes and ITS2 region were employed assess metagenomes bacterial,...

Carbapenem-resistant Acinetobacter baumannii (CRAB) is designated as an urgent public health threat, both due to its remarkable multidrug resistance and propensity for clonal spread. This study aimed explore the phenotypic molecular characteristics of antimicrobial in CRAB isolates (n = 73) from intensive care unit (ICU) patients two university hospitals Bulgaria (2018-2019). The methodology included susceptibility testing, PCR, whole-genome sequencing (WGS), phylogenomic analysis. rates...

The present study aimed to explore the genomic characteristics of eight New Delhi metallo-β-lactamase-1 (NDM-1)-producing carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates from a Bulgarian tertiary hospital (2021-2023) in comparison blaNDM-1-positive strains originating Balkans. Antimicrobial susceptibility testing, phenotypic assays for carbapenemase activity, PCR screening, whole-genome sequencing (WGS), and phylogenomic analysis were performed. Seven CRPA investigated (Minimum...

An amplicon-based metagenomic survey of archaea, fungi, and bacteria was performed on Livingston Island, Maritime Antarctica. In many the samples, patterns antagonism between these three superkingdoms were observed in form an inversely proportional dependence richnesses types microorganisms. The quantified—based numbers total tags operational taxonomic units (OTUs), four alpha diversity parameters—using Shannon, Simpson, Chao1, ACE indices. We found that most discriminative results measuring...

The present study aimed to explore the genotypic and phenotypic characteristics of biofilm formation in Bulgarian nosocomial Stenotrophomonas maltophilia isolates (n = 221) during period 2011-2022, by screening for presence biofilm-associated genes (BAG) (spgM, rmlA rpfF), their mutational variability, assessment adherent growth on a polystyrene surface. methodology included: PCR amplification, whole-genome sequencing (WGS) crystal violet microtiter plate assay quantification. overall...

Wastewater surveillance for monitoring the spread of SARS-CoV-2 remains important even in current endemic stage COVID-19 outbreak. This approach has already demonstrated its value by providing early warnings coronavirus different communities. The aim present publication is to share relevant experience from Center Competence “Clean&amp;Circle”, obtained development an effective strategy detection wastewater Sofia, Bulgaria. Using four RNA concentration/extraction methods, we revealed that key...

The Bulgarian ‘mehovo sirene’ cheese is a type of regional artisanal prepared from raw ewe milk in skin bag relying on spontaneous fermentation. A metagenomics study revealed an unusual microbiome comprising beneficial bacteria such as Staphylococcus equorum , Lactococcus lactis Streptococcus thermophilus Lactobacillus delbrueckii kefiranofaciens raffinolactis Lactiplantibacillus plantarum Limosilactobacillus fermentum and Loigolactobacillus coryniformis . Our findings suggest that could...

Paenibacillus profundus YoMME was isolated from the anodic biofilm of a sediment microbial fuel cell and recognized as one few exoelectrogenic Gram-positive bacteria, capable transferring electrons extracellularly. Here, we report its draft genome sequence. The project is deposited at DDBJ/ENA/GenBank under accession number JAJNBZ000000000.

Extensively drug-resistant P. aeruginosa (XDR-PA) has been highlighted as a serious public health threat. The present study aimed to explore the genomic characteristics of two Vietnamese extended-spectrum β-lactamase-9 (VEB-9)-producing XDR-PA isolates from Bulgaria in comparison all blaVEB-9-positive strains with available genomes. designated Pae51 and Pae52 were obtained tracheobronchial aspirates intensive care unit (ICU) patients. Antimicrobial susceptibility testing, whole-genome...

ABSTRACTA collection of twenty Lactobacillus strains isolated from yoghurts coming different sources, and determined as delbrueckii by morphological, physiological biochemical tests (13), was subjected to PCR-based methods for typing. They were screened two sets Genus-specific PCR (GS-PCR) primers which confirmed their affiliation the genus Lactobacillus. Although these dairy bacteria belong same species, some them studied in a previous research (4) showed few differences growth...

In this research, we report the isolation of Enterococcus durans EDD2, which showed strong inhibitory activity against Paenibacillus larvae, causative agent American foulbrood disease (AFB). The strain was found in freshly collected pollen granules from four beehives Dushantzi village (Pirdop region, Bulgaria), and determined by polyphase approach including molecular, biochemical, physiological tests. Whole-genome sequencing Illumina HiSeq NGS platform with 2 x 150 bases paired-end reads...

Different Enterococci, isolated from starters for the production of traditional Bulgarian yellow cheese 'Kashkaval' were screened bacteriocin production, and one them, Enterococcus faecalis 3915 demonstrated activity. In this study, we investigated growth parameters producer strain as well kinetics preliminary characterisation produced named enterocin 3915. For modelling, logistic model was used, while monitored. Experiments on inducibility conducted, checked presence plasmids. The peptide...

In recent years, excessive utilization of antibiotics has led to the emergence antibiotic microbial resistance on a planetary scale. This phenomenon represents serious threat public health, as well an enormous burden for healthcare systems’ budgets worldwide. Novel, rapid and cheap methods susceptibility screening are urgently needed this obstacle be overcome. paper, we present microfluidic device on-chip testing, which allows detection within 6 hours. The design, fabrication experimental...

Among other microorganisms twenty Lactobacillus strains were isolated from home made yoghurts different sources across Bulgaria. 13 obtained prepared cow milks while the origin of another 4 sheep's milks, and 3 buffalo's milk yoghurts. The previously classified with API tests to belong species delbrueckii. In this study their growth characteristics at temperatures obtained, a simplified mathematical model was proposed.

Information about the fungal composition of bee bread, and fermentation processes to which fungi contribute significantly, is rather scarce or fragmentary. In this study, we performed an NGS-based metagenomics snapshot picture study bread in four locations Bulgaria during most active honeybee foraging period at end June 2020. The sampling were chosen differ significantly climatic conditions, landscape, anthropogenic pressure, Illumina 2 × 250 paired-end reads platform was used for amplicon...

Abstract Cefiderocol (CFDC) is a first-in-class siderophore cephalosporin with potent activity against multidrug-resistant Gram-negative bacteria including carbapenem-resistant Acinetobacter baumannii . The present study aimed to explore the CFDC resistance mechanisms of an extensively drug-resistant A. isolate from Bulgaria. Aba52 strain (designated Aba52) was obtained in 2018 blood sample critically ill patient. methodology included antimicrobial susceptibility testing, whole-genome...