A5040502525- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Cancer-related gene regulation
- RNA regulation and disease
- MicroRNA in disease regulation
- Renal and related cancers
Duke University
2023-2024
Duke University Hospital
2018-2020
Duke Medical Center
2018-2020
N 6-methyladenosine (m6A) is an abundant RNA modification which plays critical roles in function and cellular physiology. However, our understanding of how m6A spatially regulated remains limited due to a lack methods for visualizing methylated transcripts interest cells. Here, we develop DART-FISH, method situ visualization specific sites target RNAs enables simultaneous detection both m6A-modified unmodified transcript copies. We demonstrate the ability DART-FISH visualize variety mRNAs...
Embryonic interneuron development underlies cortical function and its disruption contributes to neurological disease. Yet, the mechanisms by which viable interneurons are produced from progenitors remain poorly understood. Here we demonstrate dosage dependent requirements of exon junction complex component Magoh for genesis. Conditional ablation progenitors, but not post-mitotic neurons, depletes number through adulthood, with increased severity in homozygotes. Using live imaging discover...
Abstract De novo germline mutations in the RNA helicase DDX3X account for 1-3% of unexplained intellectual disability (ID) cases females, and are associated with autism, brain malformations, epilepsy. Yet, developmental molecular mechanisms by which impair function unknown. Here we use human mouse genetics, cell biological biochemical approaches to elucidate pathogenic variants disrupt development. We report largest clinical cohort date (n=78), demonstrating a striking correlation between...