A5024668646- Glycosylation and Glycoproteins Research
- Melanoma and MAPK Pathways
- Galectins and Cancer Biology
- Protein Degradation and Inhibitors
- Biochemical and Molecular Research
- Carbohydrate Chemistry and Synthesis
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Cancer Mechanisms and Therapy
- Cardiac, Anesthesia and Surgical Outcomes
- Enhanced Recovery After Surgery
- Cellular Mechanics and Interactions
- Abdominal Surgery and Complications
- Anesthesia and Pain Management
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Metabolomics and Mass Spectrometry Studies
- PI3K/AKT/mTOR signaling in cancer
- HER2/EGFR in Cancer Research
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Mechanisms of cancer metastasis
- Child and Adolescent Health
- Proteoglycans and glycosaminoglycans research
Oslo University Hospital
2015-2021
Norwegian University of Science and Technology
2017-2021
Goodwin College
2021
University of California, San Francisco
2020
Norwegian Cancer Society
2017
// Harri M. Itkonen 1 , Saurabh S. Gorad 2,3 Damien Y. Duveau 4 Sara E.S. Martin 5 Anna Barkovskaya 2,7 Tone F. Bathen 2 Siver A. Moestue and Ian G. Mills 1,6,8 Prostate Cancer Research Group, Centre for Molecular Medicine (Norway), University of Oslo Hospitals, Gaustadalleen, Oslo, Norway Department Circulation Medical Imaging, NTNU, Trondheim, 3 St. Olavs Hospital, Division Preclinical Innovation, National Center Advancing Translational Sciences, Institutes Health, Rockville, MD, USA...
Epithelial to mesenchymal transition (EMT) has implications in tumor progression and metastasis. Metabolic alterations have been described cancer development but studies focused on the metabolic re-wiring that takes place during EMT are still limited. We performed metabolomics profiling of a breast epithelial cell line its derived phenotype create genome-scale models descriptive both lines. Glycolysis OXPHOS were higher while amino acid anaplerosis fatty oxidation fueled phenotype. Through...
Cellular senescence is a pivotal hallmark of aging, which limits lifespan and contributes to the development age-related diseases. Efforts identify senolytics - drugs that selectively eliminate senescent cells, have so far yielded candidates with limited translational potential. Here, we characterize cell surface proteomic landscape proteins are abnormally present on plasma membrane cells. Many these lysosomal enzymes, pointing exocytosis as likely mechanism leads their persistent display...
// Kotryna Seip 1 , Karianne G. Fleten 1, * Anna Barkovskaya Vigdis Nygaard Mads H. Haugen Birgit Ø. Engesæter Gunhild M. Mælandsmo 2, 3 Lina Prasmickaite Department of Tumor Biology, Oslo University Hospital, The Norwegian Radium Oslo, Norway 2 K.G. Jebsen Center for Breast Cancer Research, Institute Clinical Medicine, Faculty Pharmacy, Health Sciences, Tromsø, These authors have contributed equally to this work Correspondence to: Prasmickaite, email:...
Abstract Post-translational modification of intracellular proteins with a single N-acetylglucosamine sugar (O-GlcNAcylation) regulates signaling, proliferation, metabolism and protein stability. In breast cancer, expression the enzyme that catalyzes O-GlcNAcylation – O-GlcNAc-transferase (OGT), extent O-GlcNAcylation, are upregulated in tumor tissue, correlate cancer progression. Here we compare significance panel cells different phenotypes. We find greater dependency on OGT among...
Abstract In this study, we probed the importance of O-GlcNAc transferase (OGT) activity for survival tamoxifen-sensitive (TamS) and tamoxifen-resistant (TamR) breast cancer cells. Tamoxifen is an antagonist estrogen receptor (ERα), a transcription factor expressed in over 50% cancers. ERα-positive cancers are successfully treated with tamoxifen; however, significant number patients develop disease. We show that vitro development tamoxifen-resistance associated increased sensitivity to OGT...
Highlights•p130Cas and βIII-tubulin support PDAC growth by enhancing MYC expression•p130Cas transcriptionally regulates through SRC-p130Cas-DOCK1-RAC1-β-catenin•Microtubules post-translationally regulate stability calpains•Triple targeting of ERK/p130Cas/tubulin with ERKi KX2-391 inhibits growthSummaryTo identify therapeutic targets for KRAS mutant pancreatic cancer, we conduct a druggable genome small interfering RNA (siRNA) screen determine that suppression BCAR1 sensitizes cancer cells to...
Cellular phenotype plasticity between the epithelial and mesenchymal states has been linked to metastasis heterogeneous responses cancer therapy, remains a challenge for treatment of triple‐negative breast (TNBC). Here, we used isogenic human cell lines, D492 D492M, representing phenotypes, respectively. We employed CRISPR‐Cas9 loss‐of‐function screen targeting 2240‐gene ‘druggable genome’ identify phenotype‐specific vulnerabilities. Cells with were more vulnerable loss genes related...
To identify therapeutic targets for KRAS-mutant pancreatic cancer, we conducted a druggable genome siRNA screen and determined that suppression of BCAR1 sensitizes cancer cells to ERK inhibition. Integrative analysis genome-scale CRISPR-Cas9 screens also identified as top synthetic lethal interactor with mutant KRAS. encodes the SRC substrate p130Cas. We inhibitor-mediated p130Cas phosphorylation impairs MYC transcription through DOCK1-RAC1-beta-catenin dependent mechanism. Additionally,...
Abstract O-linked-N-acetylglucosamination (O-GlcNAcylation) is a post-translational modification that occurs on serine and threonine amino acid residues of the intracellular proteins. O-GlcNAcylation numerous targets catalyzed by single enzyme - O-GlcNAc-transferase (OGT). impacts protein activation stability thereby regulating several key biological functions, heavily involved in cancer development progression. OGT expression total are elevated many cancers compared to non-malignant...
Abstract O-linked N-acetyl-glucosamine transferase (OGT) is an enzyme that catalyzes addition of the O-GlcNAc modification to a wide range intracellular proteins. The product hexosamine biosynthetic pathway, which requires glucose and glutamine as substrates. Uptake both these nutrients often up-regulated in cancer, turn leads increase total protein O-GlcNAcylation. Increased OGT expression has also been reported most cancer types, including frequently diagnosed women, breast cancer. Many...
Abstract Phenotypic heterogeneity of cancer cells can reason diversity in therapy responses within the same tumor, which might influence overall efficacy treatment. Tumor stroma is an important contributor to intratumoral and play a significant modulatory role response/resistance. Through studies on biological mechanisms stroma-promoted resistance, novel targets could be identified for combination therapies aimed eradicate both stroma-dependent independent counterparts tumor. In this study...
Sociocultural aspect in creating architectural space of medical facilities for children with disorders and disabilities Barkovskaya A. 1 , Shagieva E. 2 (Russian Federation) Социокультурные особенности формирования архитектурного пространства для медицинской деятельности детей с различными заболеваниями Барковская А. Ю. Шагиева Е. В. (Российская Федерация) Анна Юрьевна / Anna -кандидат философских наук, доцент, кафедра философии, социологии и
Abstract To combat cancer we have to avoid development of resistant and metastatic disease. Breast cells can switch from an epithelial mesenchymal phenotype through a process called transition/EMT. Emerging evidence suggests that this is vital treatment pressure gain capacity. Furthermore, recent literature shows metabolic reprogramming essential attribute cellular plasticity. Metabolic targeting could therefore be attractive possibility prevent resistance dissemination. Here tried...