A5040226815- Legume Nitrogen Fixing Symbiosis
- Wastewater Treatment and Nitrogen Removal
- Microbial Fuel Cells and Bioremediation
- Soil Carbon and Nitrogen Dynamics
- Metalloenzymes and iron-sulfur proteins
- Agricultural Science and Fertilization
- Insect Resistance and Genetics
- Metal-Catalyzed Oxygenation Mechanisms
- Clay minerals and soil interactions
- Heavy metals in environment
- Orthodontics and Dentofacial Orthopedics
- Microbial bioremediation and biosurfactants
- Plant Micronutrient Interactions and Effects
- Phosphorus and nutrient management
- Photosynthetic Processes and Mechanisms
- Dental Radiography and Imaging
- Plant nutrient uptake and metabolism
- Hemoglobin structure and function
- Temporomandibular Joint Disorders
- Entomopathogenic Microorganisms in Pest Control
- Ammonia Synthesis and Nitrogen Reduction
- Coal and Its By-products
- Transgenic Plants and Applications
- Plant Physiology and Cultivation Studies
- Selenium in Biological Systems
Agricultural Research & Education Organization
2017-2024
Bayer (United States)
2018-2019
Soil Conservation and Watershed Management Research
2019
Monsanto (United States)
1999-2016
University of Louisville
1987-2010
Shiraz University
2002-2008
University of Georgia
1999-2000
Johns Hopkins University
1986-1999
Washington University in St. Louis
1985-1988
University of Missouri–St. Louis
1981-1986
Patatin is a nonspecific lipid acyl hydrolase that accounts for approximately 40% of the total soluble protein in mature potato tubers, and it has potent insecticidal activity against corn rootworm. We determined X-ray crystal structure His-tagged variant an isozyme patatin, Pat17, to 2.2 Å resolution, employing SeMet multiwavelength anomalous dispersion (MAD) phasing methods. The patatin three molecules asymmetric unit, R-factor 22.0%, Rfree 27.2% (for 10% data not included refinement)...
We report the identification and characterization of a low tocopherol Arabidopsis thaliana mutant, vitamin E pathway gene5-1 (vte5-1), with seed levels reduced to 20% wild type. Map-based responsible mutation identified G-->A transition, resulting in introduction stop codon At5g04490, previously unannotated gene, which we named VTE5. Complementation wild-type transgene largely restored phenotype. A knockout Synechocystis sp PCC 6803 VTE5 homolog slr1652 by 50% or more. Bioinformatic analysis...
Abstract For almost half a century, the structure of full‐length Bacillus thuringiensis ( Bt ) insecticidal protein Cry1Ac has eluded researchers, since ‐derived crystals were first characterized in 1965. Having finally solved this we report intriguing details lattice‐based interactions between toxic core and protoxin domains. The provides concrete evidence for function as an enhancer native crystal packing stability.
The peptic ulcer-causing bacterium Helicobacter pylori was found to contain an H2-uptake hydrogenase activity coupled whole cell (aerobic) respiration. localized membranes which functioned in the H2-oxidizing direction with a variety of artificial and physiological electron acceptors positive redox potential. Immunoblotting H. membrane components anti (B. japonicum) large small subunit-specific antisera identified peptides approximately 65 26 kDa respectively, genomic DNA fragments...
Summary The FeSII protein of Azotobacter vinelandii has been proposed to mediate the ‘conformational protection’ molybdenum‐dependent nitrogenase components against oxygen inactivation. We have cloned and characterized structural gene for (the fesII Iocus). Hybridization studies did not reveal presence ‐like genes in a number diverse species well‐studied nitrogen‐fixing bacteria, with exception chroococcum. fesll locus is transcriptionally expressed during both nitrogen fixing non‐nitrogen...
Abstract Most agricultural soils in Iran are usually low organic matter (OM). Therefore, increasing OM these is of great concern. Environmental pollution caused by chemical fertilizers has created an interest the integrated use wastes with inorganic fertilizers. The main purpose this greenhouse study was to evaluate impact two and phosphorus (P) on growth, elemental composition spinach (Spinacia oleracea L.) soil properties. Treatments consisted four levels municipal waste compost, MWC (0,...
Nickel is an essential component of all H2-uptake hydrogenases. A fragment DNA that complements a H2-uptake-deficient but nickel-cured mutant strain (JHK7) Bradyrhizobium japonicum was isolated and sequenced. This 4.5-kb contains four open reading frames designated as ORF1, hupN, hupO, hupP, which encode polypeptides with predicted masses 17, 40, 19, 63.5 kDa, respectively. The last three (hupNOP) are most likely organized operon putative sigma 54-type promoter. Based on its hydropathy...
We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake of R. has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen. fact, the addition sodium dithionite aerobically protein resulted in rapid loss activity. Purified more stable when stored under O2 than Ar. Sodium-chloride-washed hydrogen-oxidizing membranes solubilized Triton...
Vegetatively expressed insecticidal proteins (VIPs) produced by Bacillus thuringiensis fall into several classes of which the third, VIP3, is known for their activity against key Lepidopteran pests commercial broad acre crops and because mode action does not overlap with that crystalline proteins. The details VIP3 structure have remained obscure quarter century has passed since discovery. In present article, we report first crystal a full-length protein. Crystallization this target required...
Summary: Cytochrome bd’ has been implicated in having an important role microaerobic nitrogen fixation the enteric bacterium Klebsiella pneumoniae , where it is expressed under all conditions that permit diazotrophy. In this paper sequence of genes encoding terminal oxidase ( cydAB ) and characterization a cyd mutant are reported. The deduced amino acid sequences support proposal His 19, 186 Met 393 provide three four axial ligands to Fe haems complex. nitrogen-fixing ability was severely...
Azotobacter vinelandii is a free-living nitrogen-fixing bacterium that has one of the highest respiratory rates all aerobic organisms. Based on various physiological studies, d-type cytochrome been postulated to be terminal oxidase vigorously respiring but apparently uncoupled branch electron transport system in membranes this organism. We cloned and characterized structural genes two subunits oxidase. The deduced amino acid sequences both A. have extensive regions homology with those...
Twenty cases treated with a Bionator followed by fixed appliance therapy are compared to an untreated sample and group appliances only. The most notable differences in effects increased vertical dimension mandibular plane angle. first used, whether or fixed, produced similar changes.
Azotobacter vinelandii strains lacking the nitrogenase-protective Shethna protein lost viability upon carbon-substrate deprivation in presence of oxygen. This loss was dependent N(2)-fixing status cultures (N(2)-fixing cells viability, while non-N(2)-fixing did not) and on ambient O(2) level. Supra-atmosheric tensions (40% partial pressure) decreased viable cell number mutant further, had a slightly higher spontaneous mutation frequency than wild type high-O(2) conditions. Iron starvation...
Partial purification of a cytochrome bd complex from Azotobacter vinelandii grown under high aeration was achieved by isolating respiratory particles enriched in this hemoprotein via differential centrifugation and detergent extraction. The subsequently solubilized the inner membrane with dodecyl maltoside purified to near homogeneity DEAE-Sepharose chromatography. Sodium sulfate-polyacrylamide gel electrophoresis indicated that consisted two subunits, sizes good agreement those predicted...
The development of insect resistance to pesticides via natural selection is an acknowledged agricultural issue. Likewise, in target populations a significant challenge the durability crop traits conferring protection and has driven need for novel insecticidal proteins (IPs) with alternative mechanism action (MOA) mediated by different receptors. combination or "stacking" transgenes encoding single plant can greatly delay resistance, but requires sufficient knowledge MOA identify receptor...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe "nitrogenase-protective" FeSII protein of Azotobacter vinelandii: overexpression, characterization, and crystallizationFarhad Moshiri, Brian R. Crouse, Michael K. Johnson, Robert J. MaierCite this: Biochemistry 1995, 34, 40, 12973–12982Publication Date (Print):October 10, 1995Publication History Published online1 May 2002Published inissue 10 October 1995https://doi.org/10.1021/bi00040a007RIGHTS & PERMISSIONSArticle...
The Langmuir, Freundlich, and Temkin isotherms were fitted to copper (Cu) adsorption data of 20 highly calcareous soils Fars Province, ranging widely in relevant physical chemical characteristics. One-gram samples each soil equilibrated for 24 h with 30 mL 0.01 M CaCl2 containing 50 500 mg Cu L−1. amount retained by solids was calculated based on the difference between initial equilibrium concentrations. showed a significant fit Temkin, linear form Langmuir isotherms. equations were,...