A5031964185- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Endoplasmic Reticulum Stress and Disease
- Virus-based gene therapy research
- Microbial Metabolites in Food Biotechnology
- Fermentation and Sensory Analysis
- Studies on Chitinases and Chitosanases
- Animal Virus Infections Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Autophagy in Disease and Therapy
- Polyamine Metabolism and Applications
- Enzyme Structure and Function
- Carbohydrate Chemistry and Synthesis
- Insect and Pesticide Research
- Polysaccharides and Plant Cell Walls
- Insect symbiosis and bacterial influences
- Hemoglobin structure and function
- Phytase and its Applications
- Evolution and Genetic Dynamics
- Galectins and Cancer Biology
- Microbial Inactivation Methods
- Viral gastroenteritis research and epidemiology
Fudan University
2010-2025
Shanghai Micro Satellite Engineering Center
2018-2025
State Key Laboratory of Genetic Engineering
2012-2023
Shanghai Fudan Microelectronics (China)
2021
Institute of Ecology and Geography
2016
Qinghai University
2016
State Key Laboratory of Plateau Ecology and Agriculture
2016
Shandong University
2007
State Key Laboratory of Microbial Technology
2007
Soy leghemoglobin, when bound to heme, imparts a meat-like color and flavor can serve as substitute for animal-derived proteins. Enhancing cellular heme synthesis improves the recombinant expression of leghemoglobin in yeast. To achieve high-level A (LBA) Kluyveromyces marxianus , food-safe yeast, large-scale modules were transferred into K. using yeast artificial chromosomes (KmYACs). These contained up 8 native heterologous genes promote supply precursors downstream synthesis. Next, eight...
Interspecific hybrids can exhibit phenotypes that surpass those of their parents, known as heterosis, which is often interest for industrial applications and evolutionary genetics research. However, constructing between distantly related species, such intergeneric yeasts, presents technical challenges. In this study, we established a method to transfer individual chromosomes from Saccharomyces cerevisiae (Sc) into Kluyveromyces marxianus (Km), an emerging model bioproduction. The Sc...
Abstract Kluyveromyces marxianus is a food‐safe yeast with great potential for producing heterologous proteins. Improving the yield in K. remains challenge and incorporating large‐scale functional modules poses technical obstacle engineering. To address these issues, linear circular artificial chromosomes of (KmYACs) were constructed loaded disulfide bond formation from Pichia pastoris or . These contained up to seven genes maximum size 15 kb. KmYACs carried telomeres either Tetrahymena...
Taking into account its thermotolerance, high growth rate, and broad substrate spectrum, Kluyveromyces marxianus can be considered an ideal consolidated bioprocessing (CBP). A major obstacle to ethanol production using K. is the low of lignocellulolytic enzymes, which reduces cellulose hydrolysis production. Thus, further improvement enzyme expression secretion essential. To improve inulinase promoter signal sequence from was optimized through mutagenesis. T(-361)A mutation inside promoter,...
Aims: To characterize the duel activities of a glycosyl hydrolase family 3 β‐glucosidase/xylosidase from rumen bacterial metagenome and to investigate capabilities its β‐d‐xylosidase for saccharification hemicellulosic xylans. Methods Results: A gene RuBGX1 was cloned yak (Bos grunniens) using metagenomic technology. Recombinant RuBGX1, expressed in Escherichia coli, demonstrated high hydrolytic on both p‐nitrophenyl‐β‐d‐glucopyranoside (pNP‐Glc) p‐nitrophenyl‐β‐d‐xylopyranoside (pNP‐Xyl)...
N-glycosylation is a prevalent post-translational modification in eukaryotes, essential for regulating protein secretion. In Saccharomyces cerevisiae, glycosylation mutants have been shown to enhance the secretion of heterologous glycosylated proteins. However, whether these can also increase non-glycosylated proteins and growth defects associated with mutations be mitigated remains unclear. This study aimed characterize optimize enhanced secretory expression promising yeast host...
Abstract Background Porcine Parvovirus (PPV) is a Parvovirinae virus that can cause embryonic and fetal loss death mummification in affected pigs. Unlike conventional vaccines, virus-like particles (VLPs) inherit the natural structure of their authentic virions highly immunostimulatory induce strong humoral immune T cell responses with no risk pathogenicity. The production PPV VLPs still challenge based on traditional expression platforms due to low yields high culture costs. Kluyveromyces...
This study explored the application of
Kluyveromyces marxianus is an emerging yeast cell host for diverse products, but multiple-gene expression in K. faces challenges due to limited current knowledge of cis-regulatory elements and insertion loci. Our previous study transferred alien Saccharomyces cerevisiae chromosome I (R1) into marxianus, resulting the creation monochromosomal hybrid KS-R1. All R1 genes were actively transcribed, providing a series loci with varying transcriptional activities. Here, we explore use as novel...
The yeast Kluyveromyces marxianus is an emerging cell factory for heterologous protein biosynthesis and its use holds tremendous advantages multiple applications. However, which genes influence the productivity of desired proteins in K. has so far been investigated by very few studies. In this study, we constructed a recombinant (FIM1/Est1E), expressed ruminal feruloyl esterase Est1E as reporter. UV-60Co-γ irradiation mutagenesis was performed on recombinant, one mutant (be termed T1)...
Abstract Background Kluyveromyces marxianus is a promising cell factory for producing bioethanol and that raised demand high yield of heterologous proteins in this species. Expressions usually lead to the accumulation misfolded or unfolded lumen endoplasmic reticulum (ER) then cause ER stress. To cope with problem, group stress response target genes (ESRTs) are induced, mainly through signaling network called protein (UPR). Characterization modulation ESRTs direct optimization expressions....
Abstract Background The 5´ untranslated region (5´ UTR) plays a key role in regulating translation efficiency and mRNA stability, making it favored target genetic engineering synthetic biology. A common feature found the UTR is poly-adenine (poly(A)) tract. However, effect of poly(A) on protein production remains controversial. Machine-learning models are powerful tools for explaining complex contributions features, but incorporating features currently lacking. Thus, our goal to construct...
In recombinant protein-producing yeast strains, cells experience high production-related stresses similar to temperatures. It is possible increase protein production by enhancing thermotolerance, but few studies have focused on this topic. Here we aim identify cellular regulators that can simultaneously activate thermotolerance and yield of protein. Through screening at 46 °C, a heat-resistant Kluyveromyces marxianus (K. marxianus) strain FDHY23 isolated. also exhibits enhanced productivity...