A5018228680- Antimicrobial Resistance in Staphylococcus
- Streptococcal Infections and Treatments
- Antimicrobial Peptides and Activities
- Pneumonia and Respiratory Infections
- Probiotics and Fermented Foods
- Bacterial Infections and Vaccines
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Infective Endocarditis Diagnosis and Management
- Gut microbiota and health
- Aquaculture disease management and microbiota
- Clostridium difficile and Clostridium perfringens research
- Immune Response and Inflammation
- Bacterial biofilms and quorum sensing
Singapore Centre for Environmental Life Sciences Engineering
2017-2024
Nanyang Technological University
2017-2024
Singapore-MIT Alliance for Research and Technology
2023-2024
The contribution of biofilms to virulence and as a barrier treatment is well-established for Staphylococcus aureus Enterococcus faecalis, both nosocomial pathogens frequently isolated from biofilm-associated infections. Despite frequent co-isolation, their interactions in have not been well-characterized. We report that combination, these two species can give rise augmented biomass dependent on the activation E. faecalis aerobic respiration. In respiration requires exogenous heme activate...
Enterococcus faecalis is a Gram-positive, opportunistic, pathogenic bacterium that causes significant number of antibiotic-resistant infections in hospitalized patients. The development antibiotic resistance hospital-associated pathogens formidable public health threat. In E. and other Gram-positive pathogens, correlations exist between lipid composition resistance. Resistance to the last-resort daptomycin accompanied by decrease phosphatidylglycerol (PG) levels, whereas multiple peptide...
The cell membrane plays a pivotal role in protecting bacteria against external threats, such as antibiotics. Cationic phospholipids lysyl-phosphatidyglycerol (L-PG) resist the action of cationic antimicrobial peptides through electrostatic repulsion.
Daptomycin is a last-line antibiotic commonly used to treat vancomycin-resistant Enterococci, but resistance evolves rapidly and further restricts already limited treatment options. While genetic determinants associated with clinical daptomycin (DAP
Membrane vesicles (MVs) contribute to various biological processes in bacteria, including virulence factor delivery, antimicrobial resistance, host immune evasion and cross-species communication. MVs are frequently released from the surface of both Gram-negative Gram-positive bacteria during growth. In some genes affecting MV biogenesis have been identified, but mechanism formation is unknown. Enterococcus faecalis, a causative agent life-threatening bacteraemia endocarditis, neither...
Summary Daptomycin is a last-line antibiotic commonly used to treat vancomycin resistant Enterococci, but resistance evolves rapidly and further restricts already limited treatment options. While genetic determinants associated with clinical daptomycin (DAP R ) have been described, information on factors affecting the speed of DAP acquisition limited. The multiple peptide factor (MprF), phosphatidylglycerol modifying enzyme involved in cationic antimicrobial resistance, linked pathogens such...
Abstract The bacterial cell membrane is an interface for envelope synthesis, protein secretion, virulence factor assembly and a target host cationic antimicrobial peptides (CAMPs). To resist CAMP killing, several Gram-positive pathogens encode the multiple peptide resistance (MprF) enzyme that covalently attaches amino acids to anionic phospholipids in membrane. While E. faecalis encodes two mprF paralogs, MprF2 plays dominant role conferring killing by human β-defensin 2 (hBD-2) strain...
Abstract Membrane vesicles (MVs) contribute to various biological processes in bacteria, including virulence factor delivery, host immune evasion, and cross-species communication. MVs are frequently being discharged from the surface of both Gram-negative Gram-positive bacteria during growth. In some genes affecting MV biogenesis have been identified, but mechanism formation is unknown. Enterococcus faecalis , a causative agent life-threatening bacteraemia endocarditis, neither mechanisms nor...