A5004425590- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Fluid Dynamics and Mixing
- Protein Structure and Dynamics
- CAR-T cell therapy research
- 3D Printing in Biomedical Research
- Advanced Control Systems Optimization
- Ginger and Zingiberaceae research
- Monoclonal and Polyclonal Antibodies Research
- Bioinformatics and Genomic Networks
- Cancer, Hypoxia, and Metabolism
Bristol-Myers Squibb (United States)
2017-2023
Universidade Federal do Ceará
1991
Process intensification has shown great potential to increase productivity and reduce costs in biomanufacturing. This case study describes the evolution of a manufacturing process from conventional processing scheme at 1000-L scale (Process A, n = 5) intensified schemes both B, 8) 2000-L scales C, 3) for production monoclonal antibody by Chinese hamster ovary cell line. For upstream part process, we implemented an seed culture enhance densities step (N-1) prior bioreactor (N) using either...
Although process intensification by continuous operation has been successfully applied in the chemical industry, biopharmaceutical industry primarily uses fed-batch, rather than or perfusion methods, to produce stable monoclonal antibodies (mAbs) from Chinese hamster ovary (CHO) cells. Conventional fed-batch bioreactors may start with an inoculation viable cell density (VCD) of ~0.5 × 106 cells/mL. Increasing VCD production bioreactor (referred as N stage bioreactor) 2-10 cells/mL...
Alpinia speciosa Schum or A. nutans is a plant of the Zingiberanceae family, Known popularly as "colony" (colônia) and used diuretic to control hypertensión. We have determinated concentration Na+ K+ found in alcoholic extract tea concoction. They contained 51.0mEq Na+, 132 mEq extract, 0,0 26 tea. Phytochemical analysis leaves demonstrated presence catecquic tanins, phenols alkaloids, also some essential oils. When injected intra-peritoneally hydroalcoholic range 100 1400 mg/Kg, (or...
As a key enzyme for glycolysis, lactate dehydrogenase (LDH) remains as topic of great interest in cancer study. Though number kinetic models have been applied to describe the dynamic behavior LDH, few can reflect its actual mechanism, making it difficult explain observed substrate and competitor inhibitions at wide concentration ranges. A novel mechanistic model is developed based on enzymatic processes interactive properties LDH. Better simulation well new interactivity information...
Abstract A physics‐based model for predicting cell culture fluid properties inside a stirred tank bioreactor with embedded PID controller logic is presented. The evokes time‐accurate solution to the velocity field and overall volumetric mass transfer coefficient, as well ongoing effects of interfacial transfer, species mixing, aqueous chemical reactions. modeled system also includes direct coupling between process variables control via logic. Satisfactory agreement realized prediction...
Abstract Increasing cell culture productivity of recombinant proteins via process improvements is the primary focus for research groups within biologics manufacturing. Any recommendations to improve a manufacturing obviously must be effective, but also robust, scalable, and with product quality comparable original process. In this study, we report that three different GS −/− CHO lines developed in media containing standard concentration selection agent methionine sulfoximine (MSX), then...