A5090830410- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Cancer Research and Treatments
- Antibiotic Resistance in Bacteria
- Prostate Cancer Treatment and Research
- Mycobacterium research and diagnosis
- Pancreatic function and diabetes
- Antimicrobial Resistance in Staphylococcus
- Virus-based gene therapy research
- Bacterial Identification and Susceptibility Testing
- Respiratory viral infections research
- Animal Genetics and Reproduction
- Antibiotic Use and Resistance
- Prostate Cancer Diagnosis and Treatment
- Influenza Virus Research Studies
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Bladder and Urothelial Cancer Treatments
- Neuroendocrine Tumor Research Advances
- Multiple Myeloma Research and Treatments
- Advanced biosensing and bioanalysis techniques
- Preterm Birth and Chorioamnionitis
- Lung Cancer Research Studies
- Bacterial Infections and Vaccines
- Reliability and Agreement in Measurement
NorthShore University HealthSystem
2012-2024
University of Chicago
2013-2023
University of Illinois Chicago
2023
Evanston Hospital
2002-2020
North Shore University Hospital
2018
St. George's University
2013
Northwestern University
1990-2009
Lurie Children's Hospital
2007
Armed Forces Institute of Pathology
2001
Mayo Clinic in Arizona
2001
The oligometastasis hypothesis suggests a spectrum of metastatic virulence where some metastases are limited in extent and curable with focal therapies. A subset patients colorectal cancer achieves prolonged survival after resection liver consistent oligometastasis. Here we define three robust subtypes de novo metastasis through integrative molecular analysis. Patients exhibiting MSI-independent immune activation experience the most favorable survival. Subtypes adverse outcomes demonstrate...
ABSTRACT Rapid and accurate detection of malaria parasites in blood is needed to institute proper therapy. We developed used a real-time PCR assay detect distinguish four Plasmodium spp. that cause human disease by using single amplification reaction melting curve analysis. Consensus primers were amplify species-specific region the multicopy 18S rRNA gene, SYBR Green was for LightCycler instrument. Patient specimens infected at 0.01 0.02% parasitemia densities detected, analytical...
ABSTRACT Klebsiella pneumoniae carbapenemases (KPCs) have recently been described in Chicago, IL, especially among residents of long-term acute care hospitals (LTACHs). These patients are frequently transferred to local Chicago for higher acuity medical care, and rapid detection isolation KPC-colonized LTACH may interrupt the introduction KPCs into hospitals. We evaluated performance a real-time PCR bla KPC from enrichment broth versus direct plating rectal surveillance swabs on two...
In an effort to develop a new therapy for cancer and improve antiprogrammed death inhibitor-1 (anti-PD-1) anticytotoxic T lymphocyte-associated protein (anti-CTLA-4) responses, we have created telomerase reverse transcriptase promoter-regulated oncolytic adenovirus rAd.sT containing soluble transforming growth factor receptor II fused with human IgG Fc fragment (sTGFβRIIFc) gene. Infection of breast renal tumor cells produced sTGFβRIIFc dose-dependent cytotoxicity. immunocompetent mouse 4T1...
ABSTRACT A real-time PCR assay was developed to identify common staphylococcal species. single set of consensus primers designed amplify a portion the 16S rRNA gene, and pair fluorescence resonance energy transfer probes used species based on unique melt properties resulting from sequence variations in amplicons each Nine strains ( S. aureus , capitis epidermidis haemolyticus hominis lugdunensis schleiferi simulans warneri ) were for development. The species-specific melting profiles...
A real-time PCR assay was developed targeting the bla(KPC) responsible for Klebsiella pneumoniae carbapenemase (KPC)-mediated carbapenem resistance and validated testing colonies or enrichment broth cultures. The accurately detects KPC-containing strains with high analytical specificity sensitivity.
The suspect human hepatocarcinogen aflatoxin B1 (AFB1) is a well-known potent initiator of hepatic tumors in rainbow trout (Oncorhynchus mykiss). Both hepatocellular carcinomas and mixed hepatocellular/cholangiocellular are induced by AFB1 trout, with the form predominating. We previously isolated two c-ras genes from liver cDNA, present study we analyzed DNA 14 AFB1-induced for point mutations exon 1 both genes. Using polymerase chain reaction (PCR) oligonucleotide hybridization methods,...
We report here the development of oncolytic adenoviruses (Ads) that have reduced toxicity, enhanced tumor tropism, produce strong antitumor response, and can overcome resistance to immune checkpoint inhibitor therapy in breast cancer. shown LyP-1 receptor (p32) is highly expressed on surface cancer cells tumors from patients, increased stromal expression transforming growth factor β-1 (TGFβ-1) associated with triple-negative Therefore, we constructed Ads, AdLyp.sT mHAdLyp.sT, which...
The objective of this study was to assess whether there is PCR evidence for C. psittaci DNA in ocular adnexal lymphoma specimens collected an academic institution the U.S. This a retrospective, single-center patients from 1994 – 2004. We used 28 biopsy adult patients, 16 control with systemic lymphomas not involving adnexa, and five benign tissue samples. presence investigated by polymerase chain reaction (PCR) each group. Two different assays were utilized: (1) conventional PCR/gel based...
Abstract Detection of circulating tumor DNA (ctDNA) from plasma cell free (cfDNA) has shown promise for diagnosis, therapeutic targeting, and prognosis. This study explores ctDNA detection by next generation sequencing (NGS) associated clinicopathologic factors in patients with pancreatic adenocarcinoma (PDAC). Patients undergoing surgical exploration or resection lesions were enrolled informed consent. Plasma samples (4–6 ml) collected prior to surgery cfDNA was recovered 95 samples....
Screening assays for Neisseria gonorrhoeae exhibit low positive predictive values, particularly in low-prevalence populations. A new real-time PCR assay that detects and identifies individual spp. using melt curve analysis was compared to two previously published supplementary assays. NsppID, a 16S rRNA PCR/melt developed distinguish N. from other spp., targeting genes reportedly specific gonorrhoeae, the cppB gene porA pseudogene. total of 408 clinical specimens (324 female endocervical...
High mol. wt genomic DNA was prepared from normal hamster pancreas and the solid ascites variants of two different transplantable carcinomas, one induced by N-nitrosobis(2-oxopropyl)amine other spontaneously occurring. This transfected into NIH/3T3 cells resulted in that were capable forming tumors when injected nude mice. Analysis mouse Southern blotting revealed presence a band specific for K-ras. Polymerase chain reaction (PCR)-mediated amplification K-ras codon 12-13 region produced 117...
Abstract BACKGROUND Prostate cancer preferentially metastasizes to bone, yet little is known about the cellular and molecular factors that support this growth. Endothelial cells are likely initial contact for circulating prostate entering bone microenvironment. METHODS Using co‐culture conditioned media experiments, we studied interactions of varying aggressiveness (PC‐3 LNCaP) with marrow endothelial (HBME‐1) in collagen gels. RESULTS In co‐culture, HBME‐1 stimulated proliferation (∼90%...
The 2009 H1N1 influenza virus has rapidly spread all over the world. At this time, regions in northern hemisphere are at beginning of typical annual respiratory season, although been highly prevalent for last several months. This year, it is likely that and seasonal viruses will coexist a period time. Rapid accurate laboratory detection its subtype very important selection appropriate antiviral therapy initiation infection control measures hospitalized patients. However, with limited useful...
The emergence of a novel pandemic human strain influenza A (H1N1/09) virus in April 2009 has demonstrated the need for well-validated diagnostic tests that are broadly applicable, rapid, sensitive, and specific. analytical performance clinical validity results generated with Roche RealTime Ready Influenza A/H1N1 Detection Set using LightCycler 2.0 instrument were characterized. Analytical was assessed by processing respiratory samples spiked H1N1/09 seasonal virus, set subtypes, containing...
Genomic and personalized medicine implementation efforts have largely centered on specialty care in tertiary health systems. There are few examples of fully integrated systems that span the healthcare continuum. In 2014, NorthShore University HealthSystem launched Center for Personalized Medicine to catalyze delivery medicine. Successful required development a scalable family history collection tool, Genetic Wellness Assessment (GWA) Breast Health (BHA) tools; pharmacogenomics programming;...