A5088104705- Malaria Research and Control
- Microbial Natural Products and Biosynthesis
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Autophagy in Disease and Therapy
- Computational Drug Discovery Methods
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Toxoplasma gondii Research Studies
- Connective tissue disorders research
- Protist diversity and phylogeny
- Research on Leishmaniasis Studies
- Glycosylation and Glycoproteins Research
- Vibrio bacteria research studies
- Photosynthetic Processes and Mechanisms
Stanford University
2018-2024
Stanford Medicine
2020-2023
Duke University
2017
Endosymbiosis has driven major molecular and cellular innovations. Plasmodium spp. parasites that cause malaria contain an essential, non-photosynthetic plastid-the apicoplast-which originated from a secondary (eukaryote-eukaryote) endosymbiosis. To discover organellar pathways with evolutionary biomedical significance, we performed mutagenesis screen for essential genes required apicoplast biogenesis in falciparum. Apicoplast(-) mutants were isolated using chemical rescue permits...
The COPII coat complex, which mediates secretory cargo trafficking from the endoplasmic reticulum, is a key control point for subcellular protein targeting. Because misdirected proteins cannot function, sorting by critical establishing and maintaining normal cell tissue homeostasis. Indeed, mutations in genes cause range of human pathologies, including cranio-lenticulo-sutural dysplasia (CLSD), characterized collagen defects, craniofacial abnormalities, skeletal dysmorphology. Detailed...
We identified MMV026468 as a picomolar inhibitor of blood-stage
ABSTRACT Artemisinins are first-line treatment for malaria, prized their extremely fast reduction of parasite load in patients. New fast-acting antimalarial compounds urgently needed to counter artemisinin resistance, but the observed with artemisinins is rare among compounds. Here we show that MMV1580853 has a very vitro killing rate, comparable dihydroartemisinin. Near-complete growth inhibition was within 1 hour and dihydroartemisinin, while chloroquine, another antimalarial, showed...
Atg8 family proteins are highly conserved eukaryotic with diverse autophagy and nonautophagic functions in eukaryotes. While the structural features required for of well established, little is known about molecular changes that facilitated acquisition divergent, Atg8. The malaria parasite Plasmodium falciparum offers a unique opportunity to study because it encodes single homolog whose only essential function inheritance an unusual secondary plastid called apicoplast. Here, we used...
Plasmodium parasites, which cause malaria, and related apicomplexans are important human veterinary pathogens. These parasites represent a highly divergent understudied branch of eukaryotes, as such often defy the expectations set by model organisms. One striking example unique apicomplexan biology is apicoplast, an essential but nonphotosynthetic plastid derived from unusual secondary (eukaryote-eukaryote) endosymbiosis. Endosymbioses major driver cellular innovation, apicoplast biogenesis...
Summary Endosymbiosis has driven major molecular and cellular innovations. Plasmodium spp. parasites that cause malaria contain an essential, non-photosynthetic plastid, the apicoplast, which originated from a secondary (eukaryote-eukaryote) endosymbiosis. To discover organellar pathways with evolutionary biomedical significance, we performed mutagenesis screen for essential genes required apicoplast biogenesis in P. falciparum . Apicoplast-minus mutants were isolated using chemical rescue...
Abstract Plasmodium parasites and related apicomplexans contain an essential “complex plastid” organelle of secondary endosymbiotic origin, the apicoplast. Biogenesis this complex plastid poses a unique challenge requiring evolution new cellular machinery. We previously conducted mutagenesis screen for apicoplast biogenesis genes to discover organellar pathways with evolutionary biomedical significance. Here we validate characterize gene candidate from our screen, Pf3D7_0913500. Using...
Abstract Atg8 family proteins are highly-conserved eukaryotic with diverse autophagy and non-autophagic functions in eukaryotes. While the structural features required for conserved of well-established, little is known about molecular changes that facilitated acquisition divergent, Atg8. The malaria parasite Plasmodium falciparum offers a unique opportunity to study because it encodes single homolog whose only essential function inheritance an unusual secondary plastid called apicoplast....