A5050020792- Photoreceptor and optogenetics research
- bioluminescence and chemiluminescence research
- Neuroscience and Neural Engineering
- Molecular Communication and Nanonetworks
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Retinal Development and Disorders
- Anesthesia and Neurotoxicity Research
- Neurological Disease Mechanisms and Treatments
- Cell Image Analysis Techniques
- Traumatic Brain Injury and Neurovascular Disturbances
- Grief, Bereavement, and Mental Health
- Neuroscience and Neuropharmacology Research
- Ethics and Legal Issues in Pediatric Healthcare
- Psychosomatic Disorders and Their Treatments
- Transcranial Magnetic Stimulation Studies
- Plant and Biological Electrophysiology Studies
- Advanced Chemical Sensor Technologies
Brown University
2019-2024
Providence College
2024
Allen Institute for Brain Science
2020-2024
National Institute of Mental Health
2022-2024
University of Maryland, College Park
2022-2024
National Institutes of Health
2022-2023
University Research Co (United States)
2022
Bioluminescent optogenetics (BL-OG) offers a unique and powerful approach to manipulate neural activity both opto- chemogenetically using single actuator molecule (a LuMinOpsin, LMO). To further enhance the utility of BL-OG by improving efficacy chemogenetic (bioluminescence-driven) LMO activation. We developed novel luciferases optimized for Förster resonance energy transfer when fused fluorescent protein mNeonGreen, generating bright bioluminescent (BL) emitters spectrally tuned Volvox...
Abstract Understanding percepts, engrams and actions requires methods for selectively modulating synaptic communication between specific subsets of interconnected cells. Here, we develop an approach to control synaptically connected elements using bioluminescent light: Luciferase-generated light, originating from a presynaptic axon terminal, modulates opsin in its postsynaptic target. Vesicular-localized luciferase is released into the cleft response activity, creating real-time Optical...
The relationship between neurons' input and spiking output is central to brain computation. Studies in vitro anesthetized animals suggest that nonlinearities emerge cells' input-output (IO; activation) functions as network activity increases, yet how neurons transform inputs vivo has been unclear. Here, we characterize cortical principal activation awake mice using two-photon optogenetics. We deliver fixed at the soma while varies with sensory stimuli. find responses optogenetic are nearly...
Abstract BioLuminescent (BL) light production can modulate neural activity and behavior through co‐expressed OptoGenetic (OG) elements, an approach termed “BL‐OG.” Yet, the relationship between BL‐OG effects bioluminescent photon emission has not been characterized in vivo . Further, degree to which strictly depend on optogenetic mechanisms driven by photons is unknown. Crucial every neuromodulation method whether activator shows a dynamic concentration range driving robust, selective,...
Abstract State-of-the-art all-optical systems promise unprecedented access to neural activity in vivo , using multiphoton optogenetics allow simultaneous imaging and control of selected neurons at cellular resolution. However, achieve wide use stimulation imaging, simple strategies are needed robustly stably express opsins indicators the same cells. Here, we describe a bicistronic adeno-associated virus (AAV) that expresses both fast bright calcium indicator jGCaMP8s, soma-targeted (st)...
Abstract The relationship between neurons’ input and spiking output is central to brain computation. Studies in vitro anesthetized animals suggest nonlinearities emerge cells’ input-output (activation) functions as network activity increases, yet how neurons transform inputs vivo has been unclear. Here, we characterize cortical principal activation awake mice using two-photon optogenetics. We deliver fixed at the soma while varies with sensory stimuli. find responses optogenetic are nearly...
In vivo fluorescence miniature microscopy has recently proven a major advance, enabling cellular imaging in freely behaving animals. However, suffers from autofluorescence, phototoxicity, photobleaching and non- homogeneous illumination artifacts. These factors limit the quality time course of data collection. Bioluminescence provides an alternative kind activity-dependent light indicator. Bioluminescent calcium indicators do not require input, instead generating photons through...
Abstract Significance Bioluminescent optogenetics (BL-OG) offers a unique and powerful approach to manipulate neural activity both opto- chemogenetically using single actuator molecule (a LuMinOpsin, LMO). Aim To further enhance the utility of BL-OG by improving efficacy chemogenetic (bioluminescence- driven) LMO activation. Approach We developed novel luciferases optimized for Förster resonance energy transfer (FRET) when fused fluorescent protein mNeonGreen, generating bright...
SignificancePain comprises a complex interaction between motor action and somatosensation that is dependent on dynamic interactions the brain spinal cord. This makes understanding pain particularly challenging as it involves rich many circuits (e.g., neural vascular) signaling cascades throughout body. As such, experimentation single region may lead to an incomplete potentially incorrect of crucial underlying mechanisms.AimWe aimed develop validate tools enable detailed extended observation...
Abstract Fluorescence miniature microscopy in vivo has recently proven a major advance, enabling cellular imaging freely behaving animals. However, fluorescence suffers from autofluorescence, phototoxicity, photobleaching and non-homogeneous illumination artifacts. These factors limit the quality time course of data collection. Bioluminescence provides an alternative kind activity-dependent light indicator. Bioluminescent calcium indicators do not require input, instead generating photons...
Abstract Significance Pain is comprised of a complex interaction between motor action and somatosensation that dependent on dynamic interactions the brain spinal cord. This makes understanding pain particularly challenging as it involves rich many circuits (e.g., neural vascular) signaling cascades throughout body. As such, experimentation single region may lead to an incomplete potentially incorrect crucial underlying mechanisms. Aim Here, we aimed develop validate new tools enable detailed...
ABSTRACT BioLuminescent (BL) light production can modulate neural activity and behavior through coexpressed OptoGenetic (OG) elements, an approach termed ‘BL-OG’. Yet, the relationship between BL-OG effects bioluminescent photon emission has not been characterized in vivo . Further, degree to which strictly depend on optogenetic mechanisms driven by photons is unknown. Crucial every neuromodulation method whether activator shows a dynamic concentration range driving robust, selective,...
Abstract Patterns of activity across many neurons are fundamental units neural computation. Two-photon holographic photostimulation allows both delivering input to, and imaging responses from, patterns or populations neurons. However, to make this method an easily-deployable tool, simple methods needed robustly stably express opsins indicators in the same cells. Here we describe a bicistronic adeno-associated virus (AAV) that transfected cells expresses fast bright calcium indicator GCaMP8s,...
Abstract Understanding percepts, engrams and actions requires methods for selectively modulating synaptic communication between specific subsets of interconnected cells. Here, we develop an approach to control synaptically connected elements using bioluminescent light: Luciferase-generated light, originating from a presynaptic axon terminal, modulates opsin in its postsynaptic target. Vesicular-localized luciferase is released into the cleft response activity, creating real-time ‘Optical...