A5077759382- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Plant-Microbe Interactions and Immunity
- Toxin Mechanisms and Immunotoxins
- HIV Research and Treatment
- CRISPR and Genetic Engineering
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Cellular transport and secretion
- Mosquito-borne diseases and control
- RNA Interference and Gene Delivery
- Protein Tyrosine Phosphatases
- Molecular Biology Techniques and Applications
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Microbial Natural Products and Biosynthesis
- RNA and protein synthesis mechanisms
- PI3K/AKT/mTOR signaling in cancer
- GABA and Rice Research
- Virology and Viral Diseases
Johns Hopkins University
2018-2022
San Diego State University
2013-2015
Yeast WHI2 was originally identified in a genetic screen for regulators of cell cycle arrest and later suggested to function general stress responses. However, the Whi2 is unknown. has predicted structure sequence similarity human KCTD family proteins, which have been implicated several cancers are causally associated with neurological disorders but largely uncharacterized. The identification conserved functions between these yeast proteins may provide insight into disease mechanisms. We...
Unicellular eukaryotes have been suggested as undergoing self-inflicted destruction. However, molecular details are sparse compared with the mechanisms of programmed/regulated cell death known for human cells and animal models. Here, we report a pathway in Saccharomyces cerevisiae leading to vacuole/lysosome membrane permeabilization. Following transient stimulus, yeast die slowly over several hours, consistent an ongoing dying process. A genome-wide screen death-promoting factors identified...
Abstract The discovery of the green fluorescent protein from Aequorea victoria has revolutionized field cell and molecular biology. Since its a growing panel proteins, fluorophores fluorescent‐coupled staining methodologies, have expanded analytical capabilities flow cytometry. Here, we exploit power genetic engineering to barcode individual cells with genes encoding proteins. For engineering, utilize retroviral technology, which allows for expression ectopic information in stable manner...
The classical secretory pathway is essential for the transport of a host proteins to cell surface and/or extracellular matrix. While well-established, many factors still remain be elucidated. One most relevant biological processes that occur during involves cleavage pro-proteins by enzymes residing in endoplasmic reticulum/Golgi/TransGolgi Network compartment. Teasing out requirements involved and would shed new light into mis-regulation leading disease. Current methodologies fail link at...
The DenV pre-membrane protein (prM) is a crucial chaperone for the viral envelope protein, preventing premature fusion with vesicles during export. prM molecules in immature particles are cleaved by host proteases, leading to mature fusogenic virions. Blockade of cleavage would restrict and represents novel druggable opportunity against DenV. We have thus established cell-based platform monitor processing that relies on an engineered two-tag scaffold travels cell surface through secretory...
Fluorescent proteins, fluorescent dyes and fluorophores in general have revolutionized the field of molecular cell biology. In particular, discovery proteins their genes enabled engineering protein fusions for localization, analysis transcriptional activation translation interest, or tracking individual cells populations. The use combination with retroviral technology has further allowed expression these mammalian a stable reliable manner. Shown here is how one can utilize to give within...
Eukaryotic ribosome assembly is an intricate process that involves four ribosomal RNAs, 80 proteins, and over 200 biogenesis factors take part in numerous interdependent steps. This complexity creates a large genetic space which pathogenic mutations can occur. Dead-end intermediates result from errors are rapidly degraded, affirming the existence of quality control pathway(s) monitor assembly. However, differentiate between on-path dead-end unknown. We engineered system to perturb human...
Fluorescent proteins, fluorescent dyes and fluorophores in general have revolutionized the field of molecular cell biology. In particular, discovery proteins their genes enabled engineering protein fusions for localization, analysis transcriptional activation translation interest, or tracking individual cells populations. The use combination with retroviral technology has further allowed expression these mammalian a stable reliable manner. Shown here is how one can utilize to give within...
Abstract Unicellular eukaryotes are suggested to undergo self-inflicted destruction. However, molecular details sparse by comparison the mechanisms of cell death known for human cells and animal models. Here we report a pathway in Saccharomyces cerevisiae leading vacuole/lysosome membrane permeabilization death. Following exposure heat-ramp conditions, model environmental stress, observed that yeast occurs over several hours, suggesting an ongoing dying process. A genome-wide screen...