A5008796953- Amyotrophic Lateral Sclerosis Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Neurological diseases and metabolism
- RNA Research and Splicing
- Anesthesia and Neurotoxicity Research
- Genomics and Chromatin Dynamics
- Immune cells in cancer
- SARS-CoV-2 and COVID-19 Research
- Genetic Neurodegenerative Diseases
- RNA modifications and cancer
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Alzheimer's disease research and treatments
- Prion Diseases and Protein Misfolding
- Cancer-related gene regulation
- Neurogenetic and Muscular Disorders Research
- Poxvirus research and outbreaks
- Neuroscience and Neuropharmacology Research
- CRISPR and Genetic Engineering
- Skin and Cellular Biology Research
- Glioma Diagnosis and Treatment
- RNA regulation and disease
- Vascular Malformations Diagnosis and Treatment
- Pluripotent Stem Cells Research
Public Health Agency of Sweden
2024
Karolinska University Hospital
2010-2021
Queen Mary University of London
2009-2020
Karolinska Institutet
2003-2020
GlaxoSmithKline (United Kingdom)
2008
Zero to Three
2004
Recent reports have supported the existence of neural stem cells in adult mammalian CNS. Important features such are self-renewal and multipotency, i.e., they can give rise to neurons, astrocytes, oligodendrocytes thus principle replace lost Observations several animal models CNS diseases shown that by unknown mechanisms endogenous as well exogenous precursor preferentially migrate damaged areas. Microglia immunoreactive nonneural lineage resident After injury CNS, microglia rapidly...
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but extraction constitutes a major bottleneck current testing. Methodological simplification could increase diagnostic availability and efficiency, benefitting care infection control. Here, we describe methods circumventing COVID-19 testing performing RT-PCR directly...
We report genetic aberrations that activate the ERK/MAP kinase pathway in 100% of posterior fossa pilocytic astrocytomas, with a high frequency gene fusions between KIAA1549 and BRAF among these tumours. These were identified from analysis focal copy number gains at 7q34, detected using Affymetrix 250K 6.0 SNP arrays. PCR sequencing confirmed presence five KIAA1549-BRAF fusion variants, along single SRGAP3 RAF1. The resulting genes lack auto-inhibitory domains RAF1, which are replaced...
ABSTRACT Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The most widely used method of COVID-19 diagnostics a reverse transcription polymerase chain reaction (RT-PCR) assay, to detect presence SARS-CoV-2 RNA in patient samples, typically from nasopharyngeal swabs. extraction major bottleneck current testing, terms turn-around, logistics, component availability and cost, which delays or completely precludes many settings....
An unprecedented upsurge in mpox, caused by clade I of the monkeypox virus (MPXV) was noted Central Africa 2024. The first mpox case with MPXV Ib outside reported Sweden mid-August. experienced a mild disease course after travelling to an affected country. No additional cases this have been detected Sweden. Strengthened public health measures and surveillance including whole genome sequencing are crucial prevent establishment novel clades.
Article18 September 2020Open Access Transparent process Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates Johan Aarum Corresponding Author [email protected] orcid.org/0000-0001-9783-0964 Barts The London School Medicine Dentistry, Blizard Institute, Queen Mary University London, UK Search for more papers by this author Claudia P Cabrera orcid.org/0000-0002-2205-5315 NIHR Cardiovascular Biomedical Research Centre, William Harvey Tania A Jones...
Neural stem cells have a broad differentiation repertoire during embryonic development and can be reprogrammed to pluripotency comparatively easily. We report that adult neural at very high efficiency monocytes, differentiated fate of an unrelated somatic lineage, by ectopic expression the Ets transcription factor PU.1. The display marker profile functional characteristics monocytes integrate into tissues after transplantation. failure reprogram lineage-committed with PU.1 suggests are...
Protein aggregation in biofluids is a poorly understood phenomenon. Under normal physiological conditions, fluid-borne aggregates may contain plasma or cell proteins prone to aggregation. Recent observations suggest that neurofilaments (Nf), the building blocks of neurons and biomarker neurodegeneration, are included high molecular weight complexes circulation. The composition these Nf-containing hetero-aggregates (NCH) change systemic organ-specific pathologies, providing basis develop...
BORIS (CTCFL) is the paralog of CTCF (CCCTC-binding factor; NM_006565), a ubiquitously expressed DNA-binding protein with diverse roles in gene expression and chromatin organisation. have virtually identical zinc finger domains, yet display major differences their respective C- N-terminal regions. Unlike CTCF, has been reported only testis certain malignancies, leading to its classification as "cancer-testis" antigen. However, pattern both significant unresolved question field DNA binding...
Plasma proteome composition reflects the inflammatory and metabolic state of organism can be predictive system-level organ-specific pathologies. Circulating protein aggregates are enriched with neurofilament heavy chain-axonal proteins involved in brain aggregate formation recently identified as biomarkers fatal neuromuscular disorder amyotrophic lateral sclerosis. Using unbiased proteomic methods, we have fully characterized content neuronal circulating from sclerosis patients healthy...
The proliferation and differentiation of neural progenitor (NP) cells can be regulated by neurotransmitters including GABA dopamine. present study aimed to examine how these two neurotransmitter systems interact affect post-natal hippocampal NP cell in vitro. Mouse express functional GABAA receptors, which upon activation led an increase intracellular calcium levels via the opening L-type channels. Activation receptors also caused a significant decrease proliferation; effect that required...
Abstract An extensive characterization of fetal mouse brain cell aggregates has been performed using immunohistochemical and stereological methods. Single suspensions from mechanically dissociated cortex hippocampus were cultured in serum‐free, B27‐supplemented medium under constant gyratory agitation for up to 56 days. Three‐dimensional started form immediately after seeding reached a final average size 500 μm diameter. Among the types identified, neurons most abundant cells aggregates,...
Activation of the major histocompatibility complex (MHC) by interferon-gamma (IFN−γ) is a fundamental step in adaptive immune response to pathogens. Here, we show that reorganization chromatin loop domains MHC evident within first 30 min IFN−γ treatment fibroblasts, and further dynamic alterations occur up 6 h. These very rapid changes at genomic sites which are occupied CTCF close IFN−γ-inducible genes. Early responses thus initiated independently CIITA, master regulator class II genes...
BORIS (CTCFL), a paralogue of the multifunctional and ubiquitously expressed transcription factor CTCF, is best known for its role in transcriptional regulation. In nucleus, particularly enriched nucleolus, crucial compartment ribosomal RNA metabolism. However, little about cytoplasmic BORIS, which represents major pool protein.We show, firstly, that has putative nuclear export signal C-terminal domain. Furthermore, associates with mRNA both neural stem cells young neurons. The majority...
ABSTRACT Most proteins in cell and tissue lysates are soluble. Here, we show that many of these proteins, including several implicated neurodegenerative diseases, maintained a soluble functional state by association with endogenous RNA, as degradation RNA invariably leads to protein aggregation. We identify the importance nucleic acid structure, single-stranded pyrimidine-rich bulges or loops surrounded double-stranded regions being particularly efficient this role, revealing an apparent...
Abstract Blood-based biomarkers can be informative of brain disorders where protein aggregation play a major role. The proteome plasma and circulating aggregates (CPA) reflect the inflammatory metabolic state organism predictive system-level and/or organ-specific pathologies. CPA are enriched with heavy chain neurofilaments (NfH), key axonal constituents involved in formation fatal neurodegenerative disorder amyotrophic lateral sclerosis (ALS). Here we show that (BPA) from ALS differ...
Abstract Background: plasma proteins composition reflects the inflammatory and metabolic state of an organism can be predictive system-level organ-specific pathologies. Circulating protein aggregates (CPA) are enriched with heavy chain neurofilaments (NfH), axonal involved in brain (BPA) formation recently identified as biomarkers fatal neuromuscular disorder amyotrophic lateral sclerosis (ALS). Methods: here we use mass spectrometry brain-enhanced TMTcalibrator™-based proteomics to evaluate...