A5080900075- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- Histone Deacetylase Inhibitors Research
- Metabolism, Diabetes, and Cancer
- RNA modifications and cancer
- Erythrocyte Function and Pathophysiology
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Protein Structure and Dynamics
Helmholtz Zentrum München
2017-2023
Jagiellonian University
2016
Cancer cells convert more glucose into lactate than healthy cells, what results in their growth advantage. Pyruvate kinase (PK) is a key rate limiting enzyme this process, makes it promising potential therapeutic target. However, currently still unclear consequences the inhibition of PK has on cellular processes. Here, we systematically investigate depletion for gene expression, histone modifications and metabolism. Epigenetic, transcriptional metabolic targets were analysed different animal...
This work focuses on the pathogenic missense mutation in YY1 protein correlated with insulinomas. Based vitro studies, we demonstrate that does not affect secondary structure of either zinc fingers or N-terminal fragment (NTF) protein. Apart from a slight increase protein's compactness, no changes tertiary were observed. The introduced significantly alters DNA-binding properties, both affinity and enthalpy-entropy contribution process, which are highly dependent recognized sequence. Obtained...