A5075264702- Metabolomics and Mass Spectrometry Studies
- Cancer Immunotherapy and Biomarkers
- Phagocytosis and Immune Regulation
- Liver Disease Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Immune cells in cancer
- PARP inhibition in cancer therapy
- Advanced Chemical Sensor Technologies
- Nutrition, Genetics, and Disease
- Bioinformatics and Genomic Networks
- Isotope Analysis in Ecology
- Mass Spectrometry Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Cancer, Lipids, and Metabolism
- Lipid metabolism and biosynthesis
- Ferroptosis and cancer prognosis
- Water Quality Monitoring and Analysis
- Cancer Research and Treatments
- Gut microbiota and health
- Fault Detection and Control Systems
- Telomeres, Telomerase, and Senescence
- Nanoplatforms for cancer theranostics
- Pancreatic function and diabetes
Universitat Rovira i Virgili
2015-2025
Institut d'Investigació Sanitària Pere Virgili
2015-2025
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2015-2025
Instituto de Salud Carlos III
2016-2025
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition
2015-2021
Centre for Omic Sciences
2015
Fred Hutch Cancer Center
2012
University of Washington
2012
University of Nebraska Medical Center
2012
University of California, Irvine Medical Center
2012
Abstract Transient reprogramming by the expression of OCT4, SOX2, KLF4 and MYC (OSKM) is a therapeutic strategy for tissue regeneration rejuvenation, but little known about its metabolic requirements. Here we show that OSKM in mice causes global depletion vitamin B 12 molecular hallmarks methionine starvation. Supplementation with increases efficiency both cultured cells, latter indicating cell-intrinsic effect. We epigenetic mark H3K36me3, which prevents illegitimate initiation...
Studying the flow of chemical moieties through complex set metabolic reactions that happen in cell is essential to understanding alterations homeostasis occur disease. Recently, LC/MS-based untargeted metabolomics and isotopically labeled metabolites have been used facilitate unbiased mapping pathways. However, due complexity resulting experimental data sets few computational tools are available for analysis. Here we introduce geoRge, a novel approach capable analyzing LC/MS from stable...
Abstract Motivation The analysis of biological samples in untargeted metabolomic studies using LC-MS yields tens thousands ion signals. Annotating these features is the utmost importance for answering questions as fundamental as, e.g. how many metabolites are there a given sample. Results Here, we introduce CliqueMS, new algorithm annotating in-source LC-MS1 data. CliqueMS based on similarity between coelution profiles and therefore, opposed to most methods, allows annotation single...
Abstract Transforming Growth Factor beta (TGF-β) induces tumor cell migration and invasion. However, its role in inducing metabolic reprogramming is poorly understood. Here we analyzed the profile of hepatocellular carcinoma (HCC) cells that show differences TGF-β expression. Oxygen consumption rate (OCR), extracellular acidification (ECAR), metabolomics transcriptomics were performed. Results indicated switch from an epithelial to a mesenchymal/migratory phenotype HCC characterized by...
Upregulation of fatty acid synthase (FASN) is a common event in cancer, although its mechanistic and potential therapeutic roles are not completely understood. In this study, we establish key role FASN during transformation. required for eliciting the anaplerotic shift Krebs cycle observed cancer cells. However, main to consume acetyl-CoA, which unlocks isocitrate dehydrogenase (IDH)-dependent reductive carboxylation, producing power necessary quench reactive oxygen species (ROS) originated...
Mitochondria constantly undergo fusion and fission events, referred as mitochondrial dynamics, which determine architecture bioenergetics. Cultured cell studies demonstrate that dynamics are acutely regulated by phosphorylation of the orchestrator dynamin-related protein 1 (Drp1) at S579 or S600. However, physiological impact crosstalk these sites is poorly understood. Here, we describe functional interrelation between S600 in vivo their role on remodeling. Mice carrying a homozygous Drp1...
Obesity is a chronic and complex disease, with an increasing incidence worldwide that associated metabolic disorders such as type 2 diabetes mellitus (T2DM). Thus, it important to determine the differences between metabolically healthy obese individuals those disorders. The aim of this study was perform untargeted metabolomics assay in women morbid obesity (MO) compared normal weight group, differentiate metabolome these MO who present T2DM. We carried out liquid chromatography-mass...
In this study, we evaluated the lipidome alterations caused by type 1 diabetes (T1D) and 2 (T2D), determining lipids significantly associated with overall in both sexes, glycaemic state.
Abstract Anti-cancer therapies can induce cellular senescence, which is highly stable, or drug-tolerant persistence, efficiently reversed upon therapy termination. While approaches to target senescent cells have been extensively studied, further understanding of the processes regulating persistence needed develop treatment strategies suppress persister cell survival. Here, we used mTOR/PI3K inhibition and characterize a model persistence-associated arrest in human cancer various origins....
There is a phenotype of obese individuals termed metabolically healthy that present reduced cardiometabolic risk. This offers valuable model for investigating the mechanisms connecting obesity and metabolic alterations such as Type 2 Diabetes Mellitus (T2DM). Previously, in an untargeted metabolomics analysis cohort morbidly women, we observed different lipid metabolite pattern between morbid those with associated T2DM. To validate these findings, have performed complementary study...
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<p>Survival genes identified by whole genome CRISPR/Cas9 screening in INK128-treated mESCs and vehicle mESCs.</p>
<p>Indexed primers for CUT&Tag library preparation</p>
<p>Oligos sequences for Tn5 production</p>
<p>Supplementary figure 6</p>
<p>Differentially expressed genes commonly upregulated in INK128 treated A549 and SK-Mel-147 cells with a p value < 0.05.</p>
<p>Supplementary figure 8</p>
<p>Differentially expressed genes commonly downregulated in INK128 treated A549 and SK-Mel-147 cells with a p value <0.05.</p>
<div>Abstract<p>Anticancer therapies can induce cellular senescence or drug-tolerant persistence, two types of proliferative arrest that differ in their stability. While is highly stable, persister cells efficiently resume proliferation upon therapy termination, resulting tumor relapse. Here, we used an ATP-competitive mTOR inhibitor to and characterize persistence human cancer various origins. Using this model previously described models senescence, compared the same cell lines...
<p>Supplementary figure 2</p>
<p>Supplementary figure 7</p>