A5090085721- Metabolomics and Mass Spectrometry Studies
- Antibiotics Pharmacokinetics and Efficacy
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Pharmaceutical and Antibiotic Environmental Impacts
- Epigenetics and DNA Methylation
- Pesticide Residue Analysis and Safety
- Analytical Chemistry and Chromatography
- Biochemical and Molecular Research
- Analytical chemistry methods development
- Gut microbiota and health
- Amino Acid Enzymes and Metabolism
- Genetics and Neurodevelopmental Disorders
- ATP Synthase and ATPases Research
- Mass Spectrometry Techniques and Applications
- Advanced Chemical Sensor Technologies
- Cancer, Lipids, and Metabolism
- interferon and immune responses
- Antibiotic Resistance in Bacteria
- Adenosine and Purinergic Signaling
- Diet and metabolism studies
- Histone Deacetylase Inhibitors Research
- Metabolism and Genetic Disorders
- Cytomegalovirus and herpesvirus research
- Diet, Metabolism, and Disease
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2019-2025
Instituto de Salud Carlos III
2020-2025
Universitat Rovira i Virgili
2017-2025
Universitat de Barcelona
2011-2024
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition
2020-2024
Institut d'Investigació Sanitària Pere Virgili
2019-2023
Abstract Transient reprogramming by the expression of OCT4, SOX2, KLF4 and MYC (OSKM) is a therapeutic strategy for tissue regeneration rejuvenation, but little known about its metabolic requirements. Here we show that OSKM in mice causes global depletion vitamin B 12 molecular hallmarks methionine starvation. Supplementation with increases efficiency both cultured cells, latter indicating cell-intrinsic effect. We epigenetic mark H3K36me3, which prevents illegitimate initiation...
Abstract Transforming Growth Factor beta (TGF-β) induces tumor cell migration and invasion. However, its role in inducing metabolic reprogramming is poorly understood. Here we analyzed the profile of hepatocellular carcinoma (HCC) cells that show differences TGF-β expression. Oxygen consumption rate (OCR), extracellular acidification (ECAR), metabolomics transcriptomics were performed. Results indicated switch from an epithelial to a mesenchymal/migratory phenotype HCC characterized by...
Upregulation of fatty acid synthase (FASN) is a common event in cancer, although its mechanistic and potential therapeutic roles are not completely understood. In this study, we establish key role FASN during transformation. required for eliciting the anaplerotic shift Krebs cycle observed cancer cells. However, main to consume acetyl-CoA, which unlocks isocitrate dehydrogenase (IDH)-dependent reductive carboxylation, producing power necessary quench reactive oxygen species (ROS) originated...
For millions of years, endogenous retroelements have remained transcriptionally silent within mammalian genomes by epigenetic mechanisms. Modern anticancer therapies targeting the machinery awaken retroelement expression, inducing antiviral responses that eliminate tumors through mechanisms not completely understood. Here, we find massive binding epigenetically activated RIG-I and MDA5 viral sensors promotes ATP hydrolysis depletes intracellular energy, driving tumor killing independently...
Abstract Multinucleated giant cells (MGCs) are implicated in many diseases including schistosomiasis, sarcoidosis and arthritis. MGC generation is energy intensive to enforce membrane fusion cytoplasmic expansion. Using receptor activator of nuclear factor kappa-Β ligand (RANKL) induced osteoclastogenesis model formation, here we report RANKL cellular programming requires extracellular arginine. Systemic arginine restriction improves outcome multiple murine arthritis models its removal...
Mitochondrial metabolism and the generation of reactive oxygen species (ROS) contribute to acquisition DNA mutations genomic instability in cancer. How influences metabolic capacity cancer cells is nevertheless poorly understood. Here, we show that homologous recombination-defective (HRD) cancers rely on oxidative supply NAD+ ATP for poly(ADP-ribose) polymerase (PARP)-dependent repair mechanisms. Studies breast ovarian HRD models depict a shift includes enhanced expression phosphorylation...
Mitochondria constantly undergo fusion and fission events, referred as mitochondrial dynamics, which determine architecture bioenergetics. Cultured cell studies demonstrate that dynamics are acutely regulated by phosphorylation of the orchestrator dynamin-related protein 1 (Drp1) at S579 or S600. However, physiological impact crosstalk these sites is poorly understood. Here, we describe functional interrelation between S600 in vivo their role on remodeling. Mice carrying a homozygous Drp1...
We report the in vivo regulation of Inosine-5´-monophosphate dehydrogenase 1 (IMPDH1) retina. IMPDH1 catalyzes rate-limiting step de novo synthesis guanine nucleotides, impacting cellular pools GMP, GDP and GTP. Guanine nucleotide homeostasis is central to photoreceptor cells, where cGMP signal transducing molecule light response. Mutations lead inherited blindness. unveil a light-dependent phosphorylation retinal at Thr159/Ser160 Bateman domain that desensitizes enzyme allosteric inhibition...
Abstract CD98 heavy chain (CD98hc) forms heteromeric amino acid (AA) transporters by interacting with different light chains. Cancer cells overexpress CD98hc-transporters in order to meet their increased nutritional and antioxidant demands, since they provide branched-chain AA (BCAA) aromatic (AAA) availability while protecting from oxidative stress. Here we show that BCAA AAA shortage phenocopies the inhibition of mTORC1 signalling, protein synthesis cell proliferation caused CD98hc...
Cataract, the loss of ocular lens transparency, accounts for ~50% worldwide blindness and has been associated with water solute transport dysfunction across cellular barriers. We show that neutral amino acid antiporter LAT2 (Slc7a8) uniporter TAT1 (Slc16a10) are expressed on mouse ciliary epithelium also in epithelium. Correspondingly, deletion induced a dramatic decrease essential levels was modulated by defect. Interestingly, absence led to increased incidence cataract mice, particular...
Machine learning tools have become essential for annotating the vast number of unidentified MS/MS spectra in metabolomics, addressing limitations current reference spectral libraries. However, these often struggle with high dimensionality and sparsity metabolite structures. ChemEmbed introduces a novel approach by combining multidimensional continuous vector representations chemical structures enhanced spectra. This enhancement is achieved merging from multiple collision energies...
The presence of residues antibiotics, metabolites, and thermal transformation products (TPs), produced during treatment to eliminate pathogenic microorganisms in milk, could represent a risk for people. Cow's milk samples spiked with enrofloxacin (ENR), ciprofloxacin (CIP), difloxacin (DIF), sarafloxacin (SAR) from cows medicated ENR were submitted several treatments. analyzed by liquid chromatography–mass spectrometry (LC-MS) find identify TPs metabolites. In this work, 27 4 quinolones 24...
Adenylosuccinate lyase (ADSL) functions in de novo purine synthesis (DNPS) and the nucleotide cycle. ADSL deficiency (ADSLD) causes numerous neurodevelopmental pathologies, including microcephaly autism spectrum disorder. ADSLD patients have normal serum levels but exhibit accumulation of dephosphorylated substrates, S-Ado, SAICAr, latter being implicated neurotoxic effects through unknown mechanisms. We examined phenotypic depletion human cells their relation to outcomes. Using specific...
The endoplasmic reticulum (ER) of cancer cells needs to adapt the enhanced proteotoxic stress associated with accumulation unfolded, misfolded, and transformation-associated proteins. One way by which tumors thrive in context ER is promoting ER-associated degradation (ERAD), although mechanisms are poorly understood. Here, we show that small p97/VCP-interacting protein (SVIP), an endogenous inhibitor ERAD, undergoes DNA hypermethylation–associated silencing tumorigenesis achieve this goal....
Isotopic-labeling experiments have been valuable to monitor the flux of metabolic reactions in biological systems, which is crucial understand homeostatic alterations with disease. Experimental determination fluxes can be inferred from a characteristic rearrangement stable isotope tracers (e.g., 13C or 15N) that detected by mass spectrometry (MS). Metabolites measured are generally members well-known pathways, and most them using both gas chromatography (GC)-MS liquid (LC)-MS. In here, we...