A5036891711- Neuroinflammation and Neurodegeneration Mechanisms
- Pluripotent Stem Cells Research
- Alzheimer's disease research and treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune cells in cancer
- Neurogenesis and neuroplasticity mechanisms
- Sphingolipid Metabolism and Signaling
- Insect and Pesticide Research
- Immune Response and Inflammation
- Neuroscience and Neural Engineering
- Plant and animal studies
- Phagocytosis and Immune Regulation
- Mitochondrial Function and Pathology
- Yersinia bacterium, plague, ectoparasites research
- Fatty Acid Research and Health
- 3D Printing in Biomedical Research
- Atherosclerosis and Cardiovascular Diseases
- S100 Proteins and Annexins
- Neuroscience and Neuropharmacology Research
- Cancer-related gene regulation
- Fire effects on ecosystems
- Cholinesterase and Neurodegenerative Diseases
- Species Distribution and Climate Change
- Tryptophan and brain disorders
- Vagus Nerve Stimulation Research
University of Saskatchewan
2022-2024
Okanagan University College
2018-2022
University of British Columbia
2018-2022
Bucknell University
2018
Human brain organoids are emerging as translationally relevant models for the study of human health and disease. However, it remains to be shown whether human-specific protein processing is conserved in organoids. Herein, we demonstrate that cell fate composition unguided dictated by culture conditions during embryoid body formation, at this stage can optimized result presence glia-associated proteins neural network activity early three-months vitro. Under these conditions, generated from...
Alzheimer’s disease (AD) is characterized by chronic neuroinflammation, which partially mediated dysregulated functions of glial cells. Cardiolipin (CL) a phospholipid normally confined to the inner mitochondrial membrane; however, it has been detected in human sera, indicating that can exist extracellular space where may interact with nearby Although CL shown modulate several microglia toll-like receptor (TLR) 4-dependent manner, effects on astrocytes are unknown. In addition their...
Alzheimer disease (AD) is a heterogenous and multifactorial disease, its pathology partly driven by microglia their activated phenotype. Brain organoids (BOs) are gaining prominence as relevant model of the human brain for study AD; however, BOs commonly devoid microglia. To overcome this limitation, current protocols incorporate through either (1) co-culture (BO co-culture), or (2) molecular manipulation at critical windows BO development to have arise innately innate cultures). It...
Background: Information presented in society meetings has not been completely vetted through a formal review process. It is entirely clear if it accurate or will ever be published peer reviewed journals.Materials and Methods: A Pubmed-Medline search was performed for all abstracts at the AHBPA from 2007-2009 Different variables including country of origin, study center, academic institution were examined to determine any could predict eventual publication.Results: 33.4% materialized into...
Abstract Background Cardiolipin (CL) is a mitochondrial membrane phospholipid that acts as signaling molecule when released into the extracellular space from degenerating and dying brain cells. Previous research has shown CL inhibits secretion of pro‐inflammatory mediators by immune‐activated microglia. alone also increases phagocytic activity microglia upregulates their expression brain‐derived neurotrophic factor (BDNF) glial cell‐derived (GDNF). The mechanisms which modulates functions,...
Summary Human brain organoids are emerging as relevant models for the study of human health and disease. However, it has not been shown whether exhibit a proteoform profile similar to brain. Herein, we demonstrate that unguided minimal batch-to-batch variability in cell composition metabolism when generated from induced pluripotent stem cells (iPSCs) derived male-female siblings. We then show profiles select proteins these more autopsied cortical cerebellar than those mouse samples. Brain...
A basic Western Blot protocol that has been optimized specifically for cell lysates.
Cardiolipin (CL) is a mitochondrial phospholipid, which has well-defined role in regulating metabolic processes and maintaining homeostasis. While CL normally confined to the inner membrane, it may be released extracellularly from damaged or dying cells during physiological pathological conditions characterized by cellular stress, tissue damage, cell death. Once released, can act as signalling molecule interacting with an autocrine paracrine manner. Previous research demonstrates that...