A5055793766- Plant Virus Research Studies
- Evolution and Genetic Dynamics
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- CRISPR and Genetic Engineering
- Plant Physiology and Cultivation Studies
- Plant Disease Resistance and Genetics
- Plant Parasitism and Resistance
- Plant-Microbe Interactions and Immunity
- Genetic Mapping and Diversity in Plants and Animals
- Cold Atom Physics and Bose-Einstein Condensates
- Legume Nitrogen Fixing Symbiosis
- Quantum chaos and dynamical systems
- Quantum, superfluid, helium dynamics
- Plant Pathogenic Bacteria Studies
- Mathematical and Theoretical Epidemiology and Ecology Models
- Advanced Chemical Sensor Technologies
- Fermentation and Sensory Analysis
- Analytical Chemistry and Chromatography
- Medicine and Dermatology Studies History
- Bacteriophages and microbial interactions
Institute for Integrative Systems Biology
2023
Universitat de València
2023
Instituto de Biología Molecular y Celular de Plantas
2007-2018
Universitat Politècnica de València
1997-2018
Consejo Superior de Investigaciones Científicas
2007-2017
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas
2009
Universidad Politécnica de Madrid
2009
Telefonica Research and Development
2009
Universidad Autónoma de Madrid
2009
Little is known about the fitness and virulence consequences of single-nucleotide substitutions in RNA viral genomes, most information comes from analysis nonrandom sets mutations with strong phenotypic effect or which have been assessed vitro, their relevance vivo being unclear. Here we used site-directed mutagenesis to create a collection 66 clones Tobacco etch potyvirus, each carrying different, randomly chosen, substitution. Competition experiments between mutant ancestral nonmutated...
ABSTRACT It has been well established that populations of RNA viruses transmitted throughout serial bottlenecks suffer from significant fitness declines as a consequence the accumulation deleterious mutations by onset Muller's ratchet. Bottlenecks are unavoidably linked to different steps infectious cycle most plant viruses, such vector-mediated transmissions and systemic colonization new leaves. Here we report evidence for in potyvirus Tobacco etch virus (TEV). TEV was inoculated into...
ABSTRACT A biotechnological application of artificial microRNAs (amiRs) is the generation plants that are resistant to virus infection. This resistance has proven be highly effective and sequence specific. However, before these transgenic can deployed in field, it important evaluate likelihood emergence resistance-breaking mutants. Two issues particular interest: (i) whether such mutants arise nontransgenic may act as reservoirs (ii) a suboptimal expression level transgene, resulting...
Tobacco etch potyvirus (TEV) has been extensively used as model system for the study of positive-sense RNA virus infecting plants. TEV ability to infect Arabidopsis thaliana varies among ecotypes. In this study, changes in gene expression A. ecotype Ler infected with have explored using long-oligonucleotide arrays. is a susceptible host that allows systemic movement, although viral load low and syndrome induced ranges from asymptomatic mild. Gene profiles were monitored whole plants 21 days...
ABSTRACT A potential drawback of recent antiviral therapies based on the transgenic expression artificial microRNAs is ease with which viruses may generate escape mutations. Using a variation classic Luria-Delbrück fluctuation assay, we estimated that spontaneous mutation rate in microRNA (amiR) target plant virus was ca. 6 × 10 −5 per replication event.
Functional redundancy, understood as the functional overlap of different genes, is a double-edge sword. At one side, it thought to serve robustness mechanism that buffers deleterious effect mutations hitting redundant copies, thus resulting in pseudogenization. other considered source genetic and innovation. In any case, genetically genes are expected show an acceleration rate molecular evolution. Here, we tackle role redundancy viral RNA genomes. To this end, have evaluated rates...
Abstract Tomato is known to be a natural and experimental reservoir host for many plant viruses. In the last few years new tobamovirus species, mottle mosaic virus (ToMMV), has been described infecting tomato pepper plants in several countries worldwide. Upon observation of symptoms growing greenhouse Valencia, Spain, we aimed ascertain etiology disease. Using standard molecular techniques, first detected positive sense single-stranded RNA as probable causal agent. Next, amplified, cloned...
We discuss several aspects of the fission phenomenon studied within a mean‐field approach with two incarnations Gogny force. Results for odd‐A nuclei obtained in framework equal‐filling approximation are discussed. also analyze impact finite but moderate temperature process and finally we properties neutron rich uranium isotopes different parametrizations force, namely D1S D1N.
Abstract Plants emit volatile organic compounds (VOCs) in response to biotic and abiotic stimuli that provide information about their physiological status other individuals the community. Nearby receivers adjust own defenses these chemical cues. The majority of studies date has concentrated on communication stressors ( e.g . salinity or drought) herbivory. Less attention had received role VOCs during microbial infections almost nothing been done for viruses. Here we investigated function...
In response to stresses, plants are capable of communicating their physiological status other individuals in the community using several chemical cues.Nearby receivers then adjust own homeostasis increase resilience.The majority studies date have concentrated on communication abiotic stressors (e.g., salinity or drought) herbivory.Less attention has been paid role during microbial infections and almost nothing focused viruses.Here we investigated effect that prevalence a turnip mosaic virus...
1 Abstract Genetic redundancy, understood as the functional overlap of different genes, is a double-edge sword. At one side, it thought to serve robustness mechanism that buffers deleterious effect mutations hitting redundant copies, thus resulting in pseudogenization. other considered source genetic and innovation. In any case, genetically genes are expected show an acceleration rate molecular evolution. Here we tackle role redundancy viral RNA genomes. To this end, have evaluated rates...