Diego B. Nóbrega

ORCID: 0000-0001-9821-1436
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About
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Research Areas
  • Milk Quality and Mastitis in Dairy Cows
  • Probiotics and Fermented Foods
  • Antibiotic Resistance in Bacteria
  • Microbial infections and disease research
  • Pharmaceutical and Antibiotic Environmental Impacts
  • Enterobacteriaceae and Cronobacter Research
  • Bacterial Identification and Susceptibility Testing
  • Salmonella and Campylobacter epidemiology
  • Bacteriophages and microbial interactions
  • Microbial Metabolism and Applications
  • Mycobacterium research and diagnosis
  • Antibiotic Use and Resistance
  • Antimicrobial Resistance in Staphylococcus
  • Identification and Quantification in Food
  • Escherichia coli research studies
  • Gut microbiota and health
  • Aquaculture disease management and microbiota
  • Vibrio bacteria research studies
  • Antibiotics Pharmacokinetics and Efficacy
  • Animal Disease Management and Epidemiology
  • Genetic and phenotypic traits in livestock
  • Digestive system and related health
  • Antimicrobial Peptides and Activities
  • Research on Leishmaniasis Studies
  • Clostridium difficile and Clostridium perfringens research

University of Calgary
2016-2025

University of Guelph
2020-2023

Center for Discovery
2022

University of Pretoria
2017-2022

Universidade Estadual de Campinas (UNICAMP)
2014-2021

Université de Montréal
2020

Calgary Laboratory Services
2017-2020

Kyoto University
2020

Département Santé Animale
2017

Universidade Estadual Paulista (Unesp)
2009-2015

We describe the global molecular epidemiology of 229 carbapenemase-producing Escherichia coli in 36 countries during 2015-2017. Common carbapenemases were oxacillinase (OXA) 181 (23%), New Delhi metallo-β-lactamase (NDM) 5 (20%), OXA-48 (17%), Klebsiella pneumoniae carbapenemase 2 (15%), and NDM-1 (10%). identified dominant sequence types (STs); 4 (ST410, ST131, ST167, ST405), 1 (ST1284) was limited to Turkey. OXA-181 frequent Jordan (because ST410-B4/H24RxC subclade) Turkey ST1284). found...

10.3201/eid2805.212535 article EN cc-by Emerging infectious diseases 2022-05-01

The effect of non-aureus staphylococci (NAS) in bovine mammary health is controversial. Overall, NAS intramammary infections (IMI) increase somatic cell count (SCC), with an categorized as mild, mostly causing subclinical or mild to moderate clinical mastitis. However, based on recent studies, specific may affect the udder more severely. Some these apparent discrepancies could be attributed large number species that compose group. objectives this study were determine (1) SCC quarters...

10.3168/jds.2016-12479 article EN publisher-specific-oa Journal of Dairy Science 2017-04-27

Emergence and spread of antimicrobial resistance is a major concern for the dairy industry worldwide. Objectives were to determine: 1) phenotypic genotypic prevalence drug-specific 25 species non-aureus staphylococci, 2) associations between presence determinants resistance. Broth micro-dilution was used determine profiles 1,702 isolates from 89 herds. Additionally, 405 sequenced screen determinants. Antimicrobial clearly species-dependent. Resistance quinupristin/dalfopristin common in...

10.3389/fmicb.2018.00256 article EN cc-by Frontiers in Microbiology 2018-02-16

The emergence of carbapenem resistance is a significant public health concern. rate infections caused by carbapenemase-producing

10.1128/spectrum.04144-22 article EN cc-by Microbiology Spectrum 2023-02-27

Non-aureus staphylococci (NAS), a heterogeneous group of large number species and subspecies, are the most frequently isolated pathogens from intramammary infections in dairy cattle. Phylogenetic relationships among bovine NAS controversial have mostly been determined based on single-gene trees. Herein, we analyzed phylogeny using whole-genome sequencing (WGS) 441 distinct isolates. In addition, evolutionary were estimated multilocus data 16S rRNA, hsp60, rpoB, sodA, tuf genes sequences...

10.3389/fmicb.2016.01990 article EN cc-by Frontiers in Microbiology 2016-12-20

Staphylococcus aureus causes persistent clinical and subclinical bovine intramammary infections (IMI) worldwide. However, there is a lack of comprehensive information regarding genetic diversity, the presence antimicrobial resistance (AMR), virulence genes for S. in milk Canada. Here, we performed whole-genome sequencing (WGS) 119 Canadian isolates determined they belonged to 8 sequence types (ST151, ST352, ST351, ST2187, ST2270, ST126, ST133, ST8), 5 clonal complexes (CC151, CC97, CC126,...

10.1128/msystems.00063-20 article EN cc-by mSystems 2020-07-06

Non- aureus staphylococci (NAS) are the most frequently isolated pathogens from milk in dairy cattle worldwide. The virulence factors (VFs) and mechanisms by which these bacteria cause udder infection not fully known. We determined distribution associations of 191 VFs 25 NAS species investigated relationship between disease. Although overall number was associated with disease severity, increasing numbers toxin host immune evasion genes specifically were more severe outcomes. These findings...

10.1128/msystems.00098-18 article EN cc-by mSystems 2019-03-04

Here we investigated the prevalence and spatial distribution of selected pathogens associated with infectious diseases dairy cattle in Ontario, Canada. The cross-sectional study surveyed bulk tank milk for antibodies against bovine leukemia virus (BLV), Mycobacterium avium ssp. paratuberculosis (MAP), Salmonella Dublin, presence mastitis (Staphylococcus aureus, Streptococcus agalactiae, Mycoplasma bovis). Between October 2021 June 2022, (BTM) samples were obtained from every commercial farm...

10.3168/jds.2023-24197 article EN cc-by Journal of Dairy Science 2024-02-29

Abstract Background Hospitals represent ideal locations for developing wastewater (WW) surveillance antibiotics (Abx), owing to the high frequency of Abx use and robust record-keeping. To investigate this technology as a potentially useful stewardship tool, we compared concentration several in WW from tertiary care hospitals their corresponding levels surrounding municipality. Concentration 4 common antimicrobials found hospital effluent over 4-month period Concentrations measured 3...

10.1093/ofid/ofae631.1968 article EN cc-by Open Forum Infectious Diseases 2025-01-29
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