A5034431754- Nanopore and Nanochannel Transport Studies
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Biosensors and Analytical Detection
- Microfluidic and Capillary Electrophoresis Applications
- Lipid Membrane Structure and Behavior
- Carbon and Quantum Dots Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Microfluidic and Bio-sensing Technologies
- Nanomaterials for catalytic reactions
- Copper-based nanomaterials and applications
- DNA and Biological Computing
- Ion-surface interactions and analysis
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Iron oxide chemistry and applications
- Protein Structure and Dynamics
- Force Microscopy Techniques and Applications
- Electrochemical Analysis and Applications
- Protein Hydrolysis and Bioactive Peptides
- RNA Interference and Gene Delivery
- Probiotics and Fermented Foods
- Advanced Photocatalysis Techniques
- Nanoparticle-Based Drug Delivery
- Dye analysis and toxicity
Vietnam National University, Hanoi
2022-2024
VNU University of Science
2023-2024
The Ohio State University
2023
University of Massachusetts Amherst
2016-2022
Abstract Bacterial conjugation mediates contact-dependent transfer of DNA from donor to recipient bacteria, thus facilitating the spread virulence and resistance plasmids. Here we describe how variants plasmid-encoded outer membrane (OM) protein TraN cooperate with distinct OM receptors in recipients mediate mating pair stabilization efficient transfer. We show that plasmid pKpQIL ( Klebsiella pneumoniae ) interacts OmpK36, plasmids R100-1 Shigella flexneri pSLT Salmonella Typhimurium)...
DNA is a promising next-generation data storage medium, but challenges remain with synthesis costs and recording latency. Here, we describe prototype of system that uses an extended molecular alphabet combining natural chemically modified nucleotides. Our results show MspA nanopores can discriminate different combinations ordered sequences nucleotides in custom-designed oligomers. We further demonstrate single-molecule sequencing the using neural network architecture classifies raw current...
We have previously shown that a biotin ligand tethered to the rim of an OmpG nanopore can be used detect biotin-binding proteins. Here, we investigate effect length polyethylene glycol tether on nanopore's sensitivity and selectivity. When was increased from 2 45 ethylene repeats, decreased substantially for neutral protein streptavidin slightly positively charged (avidin). In addition, found two distinct avidin binding conformations were possible when using long tether. These sensitive salt...
The need for analytical methods that are fast, affordable, and ecologically friendly is expanding. Because of its low solvent consumption, minimal waste production, speedy analysis, capillary electrophoresis considered a “green” choice among separation methods. With these features, we have utilized the method with capacitively coupled contactless conductivity detection (CE-C4D) to simultaneously determine glucosamine Ca2+ in dietary supplements. CE analysis was performed fused silica...
Adsorption characteristics of the antibiotic meropenem on a novel magnetic material synthesized by surface coating cobalt iron oxide (CFO) with gold nanoparticles (AuNPs) were systematically investigated. The AuNPs can enhance adsorption capacity having high affinity towards thioether and amine groups in structure. Au coverage CFO decreased saturation magnetization from 55.8 emu/g to 48.8 emu/g, still allowing CFO@Au nanomaterials be magnetically recoverable. showed enhanced 25.5 mg/g at...
Molecular detection via nanopore, achieved by monitoring changes in ionic current arising from analyte interaction with the sensor pore, is a promising technology for multiplex sensing development. Outer Membrane Protein G (OmpG), monomeric porin possessing seven functionalizable loops, has been reported as an effective platform selective protein detection. Using flow cytometry to screen unfavorable constructs, we identified two OmpG nanopores unique peptide motifs displayed either loop 3 or...
Although fundamentally significant in structural, chemical, and membrane biology, the interfacial protein-detergent complex (PDC) interactions have been modestly examined because of complicated behavior both detergents proteins aqueous phase. Membrane are prone to unproductive aggregation resulting from poor detergent solvation, but participating forces this phenomenon remain ambiguous. Here, we show that using rational protein design, targeted chemical modification, steady-state...
Understanding how membrane proteins interact with detergents is of fundamental and practical significance in structural chemical biology as well nanobiotechnology. Current methods for inspecting protein-detergent complex (PDC) interfaces require high concentrations protein are low throughput. Here, we describe a scalable, spectroscopic approach that uses nanomolar native solutions. This approach, which based on steady-state fluorescence polarization (FP) spectroscopy, kinetically resolves...
Abstract We present a novel colorimetric aptasensing method for specific adenosine (Ade) detection based on complementary aptamer hybridization and gold nanoparticles (AuNPs). Unlike traditional approaches, our overcomes limitations of selectivity specificity by incorporating an additional step involving the strand (c‐Ade aptamer) after incubating Ade with aptamer. In absence Ade, c‐Ade hybridizes aptamer, causing AuNPs aggregation blue color change. However, in presence aptamer‐bound target...
The high optical cross sections of plasmonic nanoparticles make them attractive for applications in photovoltaics and photocatalysis that seek to convert light into electrical charges. However, the ultrafast deactivation hot electrons via electron–electron scattering has limited their utilization. Here, we describe electron transfer (HET) core–shell with a gold core cerium oxide (CeO2) shell three-component hierarchical material which are paired sheets reduced graphene (rGO). In particular,...
A three-dimensional structure has garnered significant interest as a potential material for ultradurable and recyclable surface-enhanced Raman scattering (SERS). Leveraging the superior chemical physical stability, together with extensive surface area of 3D copper foam (CF), CuO nanorods were grown within CF to increase surface-active photocatalytic properties. CF@CuO-Ag was effectively fabricated boost SERS performance through deposition Ag nanostructures onto surfaces nanorods. The...
Cytolysin A (ClyA) is a water-soluble alpha pore-forming toxin that assembles to form an oligomeric pore on host cell membranes. The ClyA monomer possesses α-helical bundle with β-sheet subdomain (the β-tongue) previously believed be critical for assembly and/or insertion. Oligomerization of pores transforms the β-tongue into helix-turn-helix embeds lipid bilayer. Here, we show mutations did not prevent oligomerization or transmembrane Instead, substitution mutants yielded decreased...