A5033392239- RNA modifications and cancer
- Oral Health Pathology and Treatment
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Oral and Maxillofacial Pathology
- Cancer Immunotherapy and Biomarkers
- Head and Neck Cancer Studies
- Cancer Diagnosis and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Tumors and Oncological Cases
- Oral and gingival health research
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Bone health and treatments
- Pain Management and Treatment
- Genetic and rare skin diseases.
- Skin and Cellular Biology Research
- NF-κB Signaling Pathways
- Single-cell and spatial transcriptomics
- Congenital limb and hand anomalies
- Gastrointestinal disorders and treatments
- Neuroblastoma Research and Treatments
- Restraint-Related Deaths
- Wnt/β-catenin signaling in development and cancer
- RNA regulation and disease
Dr. R. Ahmed Dental College and Hospital
2016-2024
Leeds General Infirmary
2000
A thickened, white patch - leukoplakia in the oral cavity is usually benign, but sometimes (in ~9% of individuals) it progresses to malignant tumour. Because genomic basis this progression poorly understood, we undertook study and collected samples four tissues leukoplakia, tumour, adjacent normal, blood from each 28 patients suffering gingivobuccal cancer. We performed multiomics analysis 112 (four per patient patients) integrated information on progressive changes mutational...
Deregulation of miRNA expression may contribute to tumorigenesis and other patho-physiology associated with cancer. Using TLDA, 762 miRNAs was checked in 18 pairs gingivo buccal cancer-adjacent control tissues. Expression significantly deregulated further validated cancer examined two types precancer (leukoplakia lichen planus) tissues by primer-specific TaqMan assays. Biological implications these were assessed bioinformatically. hsa-miR-1293, hsa-miR-31, hsa-miR-31* hsa-miR-7 up-regulated...
Abstract Oral squamous cell carcinoma of the gingivo-buccal region (OSCC-GB) accounts for highest cancer morbidity and mortality among men in India. It has been observed that about one-third individuals with oral leukoplakia, a dysplastic precancerous lesion cavity, progress to cancer. We aimed identify systematic transcriptomic changes as normal tissue cavity progresses frank OSCC-GB. Seventy-two OSCC-GB patients, from multiple hospitals, were recruited, transcriptome analysis tumor...
Abstract Oral squamous cell carcinoma of the gingivo‐buccal region (OSCC‐GB) is most common cancer among men in India, and associated with poor prognosis frequent recurrence. Cellular heterogeneity OSCC‐GB was investigated by single‐cell RNA sequencing tumors derived from oral cavity 12 patients, 3 whom had concomitant presence a precancerous lesion (oral submucous fibrosis [OSMF]). Unique malignant types, features, phenotypic shifts stromal population were identified fibrosis. Expression...
Genetic variations at microRNA and processing genes are known to confer risk of cancer in different populations. Here, we studied eight (miRNA) four miRNA 452 controls 451 oral patients by TaqMan genotyping assays. Variant allele-containing genotypes mir-196a2 variant allele homozygous genotype Ran increased the significantly [adjusted odds ratio (OR) (95 % confidence interval (CI)) = 1.3 (1–1.7) 2.3 (1.1–4.6), respectively]. Conversely, mir-34b Gemin3 reduced OR CI) 0.7 (0.5–0.9) 0.6...
It is hypothesized that same driver gene mutations should be present in both oral leukoplakia and cancer tissues. So, we attempted to find out at one of the genes, CASP8, adjacent Patients (n = 27), affected by leukoplakia, were recruited for study. Blood tissue DNA samples used identify somatic CASP8 next generation sequencing method. In total, 56% (15 27) 30% (8 tissues had mutations. 8 patients, tissues, located within 2-5 cm tumor sites, identical But, 7 but none beyond 5cm Mutated...
MicroRNAs have been implicated in cancer but studies on their role precancer, such as leukoplakia, are limited. Sequence variations at eight miRNA and four processing genes were studied 452 healthy controls 299 leukoplakia patients to estimate risk of disease.Genotyping by TaqMan assay followed statistical analyses showed that variant genotypes Gemin3 mir-34b reduced disease [OR = 0.5(0.3-0.9) OR 0.7(0.5-0.9) respectively] overall well smokers 0.58(0.3-1) 0.68(0.5-0.9) respectively]. Among...
Several studies reported that mtDNA mutations may play important roles in carcinogenesis although the mechanism is not clear yet. Most of compared sequences a tumor with those normal tissues from different individuals ignoring inter-individual variations. In this study, 271 SNPs, 7 novel SNPs (or SNVs), and 15 somatic were detected 8 oral cancer respect to reference (rCRS) adjacent tissues, respectively, using Ion PGM next generation sequencing method. sequence variations (76 1 somatic) are...
Introduction Murine tumor growth restriction by neem leaf glycoprotein (NLGP) was established in various transplanted models of murine sarcoma, melanoma and carcinoma. However, the role NLGP sequential carcinogenic steps has not been explored. Thus, tongue carcinogenesis Swiss mice induced 4-nitroquinoline-1-oxide (4NQO), which close resemblance to human process. Interventional initiation-promotion protocol during 4NQO mediated relation systemic immune alteration epithelial-mesenchymal...
Calcifying epithelial odontogenic tumor (CEOT), a benign, infrequent, neoplasm, is mostly intraosseous, with occasional peripheral presentations. Although rare, the malignant transformation potential of CEOT has been reported. Herein, we elucidate four cases, involving female subjects, emphasis on relevant clinical-radiological-histopathological and immunohistochemical diagnostic features. The cases presented here showed diverse clinical, radiological histopathological spectrum, one showing...
Brown tumor is a lesion that develops in patients with hyperparathyroidism. It can be described as the terminal stage of bone remodeling process where abnormal metabolism takes place. Hyperplasia or adenoma parathyroid glands main etiology primary hyperparathyroidism (HPT). Due to overproduction hormone resorption place, which appear cyst-like lesions, commonly known osteitis fibrosa cystica brown tumor. Any skeletal bones, including jawbones affected this disorder. Most time detected after...
Abstract Background Oral squamous cell carcinoma of the gingivo-buccal region (OSCC-GB) has highest incidence among males and second overall all cancers in India, emphasizing need for precise molecular classifications to guide personalized therapy. Methods We performed bulk RNA sequencing on tumor adjacent normal tissue samples from 72 OSCC-GB patients, as well leukoplakia 25 patients with concurrent leukoplakia. Findings Our analysis revealed activated epithelial-mesenchymal transition,...
Complex regional pain syndrome (CRPS) with localized erythema and hyperthermia of skin can be a perplexing clinical situation has created varied diagnostic dilemmas for clinicians. Various cases CRPS in the extremities have been documented, but head neck region, it is quite uncommon only 13 reported since 1947 here lies importance reporting case. A 30-year-old female to OPD complain severe pain, occasional swelling, change overlying temperature, color left lower one-third region face near...
Aspiration or ingestion of foreign bodies by children is a common problem globally. Corpus alienum bodies, embedded in the palate other areas oral cavity, are unusual findings that can occasionally be muddled with lesions. Studies reveal majority cases occur children, wherein 50% lack proper history. Since infants very young fail to provide history and extremely scared repeated examination clinical diagnosis all more difficult. The risks respiratory obstruction, mucosal tear, nasopharyngeal...
Oral squamous cell carcinoma (OSCC) is a common oncological problem in India. However, the frequency of developing synchronous carcinomas, i.e., development second primary tumor (SPT) either simultaneously or within 6 months index tumor, orofacial region are rare and ranges from 8% to 21%. These lesions more aggressive, treatment-resistant, metastasize early. The onset SPT decreases 5-year survival by 18–30% as compared those with single tumor. Astonishingly, it was found that OSCCs showed...