A5064083795- Receptor Mechanisms and Signaling
- Mass Spectrometry Techniques and Applications
- Neuropeptides and Animal Physiology
- Monoclonal and Polyclonal Antibodies Research
- Lipid Membrane Structure and Behavior
- Chemokine receptors and signaling
- Photoreceptor and optogenetics research
ETH Zurich
2020-2021
Board of the Swiss Federal Institutes of Technology
2021
G protein-coupled receptors (GPCRs) are the largest class of human membrane proteins that bind extracellular ligands at their orthosteric binding pocket to transmit signals cell interior. Ligand evokes conformational changes in GPCRs trigger intracellular interaction partners (G proteins, protein kinases, and arrestins), which initiate diverse cellular responses. It has become increasingly evident preference a GPCR for certain partner is modulated by range factors, e.g., or lipids embedding...
The binding pockets of aminergic G protein-coupled receptors are often targeted by drugs and virtual screening campaigns. In order to find ligands with unprecedented scaffolds for one the best-investigated this subfamily, β2 -adrenergic receptor, we conducted a docking-based screen insisting that molecules would address previously untargeted residues in extracellular loop 2. We here report discovery undescribed coumaran-based scaffold. Furthermore, provide an analysis added value X-ray...
Molecular processes within cells have traditionally been studied with biochemical methods due to their high degree of specificity and ease use. In recent years, cell-based assays gained more popularity since they facilitate the extraction mode action, phenotypic, toxicity information. However, provide specificity, cellular rely heavily on biomolecular labels tags while label-free only offer holistic information about a bulk property investigated cells. Here, we introduce assay for...
Abstract Arrestin-1 desensitizes the activated and phosphorylated photoreceptor rhodopsin by forming transient rhodopsin−arrestin-1 complexes that eventually decay to opsin, retinal arrestin-1. Via a multi-dimensional screening setup, we identified combined arrestin-1 mutants form lasting with light-activated in harsh conditions, such as high ionic salt concentration. Two quadruple mutants, D303A + T304A E341A F375A R171A share similar heterologous expression thermo-stability levels wild...