Notch signal transduction centers on a conserved DNA-binding protein called Suppressor of Hairless [Su(H)] in Drosophila species. In the absence activation, target genes are repressed by Su(H) acting conjunction with partner, Hairless, which contains binding motifs for two global corepressors, CtBP and Groucho (Gro). Usually these corepressors thought to act via different mechanisms; complexed other transcriptional regulators, they function independently and/or redundantly. Here we have...

Abstract Glycogen synthase kinase 3β (GSK3β) plays a fundamental role during the inflammatory response induced by bacteria. Depending on pathogen and its virulence factors, type of cell probably context in which interaction between host cells bacteria takes place, GSK3β may promote or inhibit inflammation. The goal this review is to discuss recent findings inhibition activation modulation signaling monocytes/macrophages epithelial at transcriptional level, mainly through regulation nuclear...

Internalization of Staphylococcus aureus in bovine endothelial cells (BEC) is increased by tumor necrosis factor alpha stimulation and NF-κB activation. Because the phosphoinositide-3-kinase (PI3K)-Akt signaling pathway also modulates activity, we considered whether internalization S. BEC associated with activity PI3K Akt. We found a time- multiplicity infection-dependent phosphorylation Akt on Ser473 infected aureus. This was inhibited LY294002 (LY), indicating participation PI3K....

Abstract Bacterial internalization is an important process in the pathogenesis of infectious diseases which nuclear factor kappaB (NF‐κB) plays a prominent role. We present pharmacological evidence indicating that bovine endothelial cells (BEC) Staphylococcus aureus , pathogenic bacterium causes mastitis cattle, was associated with activation NF‐κB. The S. increased when BEC were stimulated alpha‐tumour necrosis (TNF‐α) or beta‐interleukin 1 (IL‐1β) are known activators SN50 (an inhibitor...

Rapid isolation and identification of pathogens is a major goal diagnostic microbiology. In order to isolate identify Staphylococcus aureus, number authors have used variety selective and/or differential culture media. However, date, there are no reports comparing the efficacy media for S. aureus from bovine mastitis cases using 16S rRNA (rrs) gene sequence as gold standard test. present study, we evaluated four milk samples collected cows suffering mastitis. Four hundred forty isolates were...

Glycogen synthase kinase 3 (GSK3) is a constitutively-active enzyme implicated in the regulation of cytokine expression and inflammatory response during bacterial infections. Mammals have two GSK3 isoforms named GSK3α GSK3β that plays different but often overlapping functions. Although role caused by bacteria well described, has not been found to participate this process. Therefore, we tested if may act as regulatory isoform bovine endothelial cells (BEC) infected with Staphylococcus aureus...

Abstract Background Bacterial infections are responsible of high economic losses in aquaculture. Mexican golden trout ( Oncorhynchus chrysogaster ) is a threatened native species that has been introduced aquaculture both for conservation and breeding production which no studies bacterial have reported. Case presentation Fish from juvenile stages showed an infectious outbreak farm co-culture with rainbow mykiss ), showing external puntiform red lesions around the mouth caudal pedunculus...

This study describes four multiple nucleocapsid nucleopolyhedrovirus isolates recovered from infected larvae of beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae), on crops in two different geographical regions Mexico. Molecular and biological characterization was compared with characterized S. (SeMNPV) the United States (SeUS1 SeUS2) Spain (SeSP2). Restriction endonuclease analysis viral DNA confirmed that all Mexican were SeMNPV isolates, but molecular differences between...

We analyze the humoral and B-cell mediated immunogenic response induced by GP5 lineal epitopes from porcine reproductive respiratory syndrome virus (PRRSV) to explore role of this protein in protective response. For inoculation, pigs aged twenty-one days were allocated into six groups (seven per group): control (PBS), vehicle (carrier), vaccinated (Ingelvac-PRRSV, MLV®), non-vaccinated naturally infected, GP5-B GP5-B3. Subsequently blood samples collected analyzed. Cytokine measured...

ABSTRACT This study aimed to investigate the immunomodulatory behavior of soluble immune checkpoints (sICPs) and other biomarkers in pathophysiology SARS-CoV-2 infection. The included 59 adult participants, 43 whom tested positive for SARS-CoV-2. Patients were divided into three cohorts: those with moderate disease ( n = 16), recovered patients severe 13), deceased 16). In addition, 16 participants pre-pandemic subjects negative relative activity neutralizing antibodies (rNAbs) against...

Glycogen synthase kinase 3 (GSK3) is a constitutively active regulatory enzyme that important in cancer, diabetes, and cardiovascular, neurodegenerative, psychiatric diseases. While GSK3α usually neurodegenerative diseases GSK3β fundamental the inflammatory response caused by bacterial components. Peptidoglycan (PGN), one of most abundant cell-wall structures Gram-positive bacteria, an inducer inflammation. To evaluate whether inhibition activity bovine endothelial cells (BEC) regulates...

Staphylococcus aureus is a pathogenic bacterium causing clinical and subclinical bovine mastitis. Infections of the udder by S. are frequently associated with presence epidermidis, an opportunistic pathogen. We reported previously that capacity endothelial cells (BEC) to endocytize activation NF-kappaB modulated proinflammatory cytokines TNF-alpha IL-1beta. In this work, we explore ability BEC eliminate intracellular epidermidis their response these cytokines. Time-kinetics survival...

We analyzed the T-cell responses induced by lineal epitopes of glycoprotein 5 (GP5) from PRRSV to explore role this protein in immunological protection mediated T-cells. The GP5 peptides were conjugated with a carrier for primary immunization and booster doses. Twenty-one-day-old pigs allocated into four groups (seven per group): control (PBS), vehicle (carrier), PTC1, PTC2. Cytokine levels measured at 2 days post-immunization (DPI) serum samples. Cytotoxic T-lymphocytes (CTLs, CD8+)...

The white-tailed deer (Odocoileus virginianus peruvianus) is a ruminant of the family Cervidae that managed as game species in Sauce Grande area Coto de Caza El Angolo (CCEA), Piura, Peru. aim this study was to propose health protocol for CCEA an essential component their management work plans well serve model other wildlife protected natural areas. For development literature review done on species, along with five visits evaluation and standardization some procedures, where biological...

The ability of nanostructured TiO2 in anatase phase to eliminate Escherichia coli (E. coli) by UV light irradiation was tested using titania films supported on glass substrates. were obtained electrophoretic deposition sol sputtered Ti Corning Experimental procedure used obtain these and their characterizations are discussed this paper. Nanostructure nature the analyzed scanning electron microscopy atomic force microscopy. Optic study photocatalytic activity interaction with E. bacteria,...

The aim of this study was to analyze the immunogenic response elicited in swine by two synthetic peptides derived from GP5 understand role lineal B epitopes humoral and B-cell-mediated against porcine reproductive respiratory syndrome virus (PRRSV). For inoculation, twenty-one-day-old pigs were allocated into six groups: control, vehicle, vaccinated (Ingelvac-PRRSV, MLV®), non-vaccinated naturally infected, GP5-B GP5-B3. At 2 days post-immunization (dpi), GP5-B3 peptide increased serum...