A5079571815- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Advanced biosensing and bioanalysis techniques
- Mass Spectrometry Techniques and Applications
- RNA Research and Splicing
- Nitrogen and Sulfur Effects on Brassica
- Lipid metabolism and biosynthesis
- Plant Reproductive Biology
- Microbial Metabolic Engineering and Bioproduction
- Pluripotent Stem Cells Research
- Protein Structure and Dynamics
- Agriculture and Biological Studies
University of Zagreb
2018-2023
Abstract Establishing causal relationship between epigenetic marks and gene transcription requires molecular tools, which can precisely modify specific genomic regions. Here, we present a modular extensible CRISPR/dCas9-based toolbox for editing direct regulation. It features system expression of orthogonal dCas9 proteins fused to various effector domains includes multi-gRNA simultaneous targeting orthologs up six loci. The C- N-terminal fusions with DNMT3A TET1 catalytic were thoroughly...
Isoleucyl-tRNA synthetase (IleRS) is an essential enzyme that covalently couples isoleucine to the corresponding tRNA. Bacterial IleRSs group in two clades, ileS1 and ileS2, latter bringing resistance natural antibiotic mupirocin. Generally, bacteria rely on either or ileS2 as a standalone housekeeping gene. However, we have found exception by noticing Bacillus species with genomic consistently also keep ileS1, which appears mandatory family Bacillaceae. Taking Priestia (Bacillus) megaterium...
Antibiotics target key biological processes that include protein synthesis. Bacteria respond by developing resistance, which increases rapidly due to antibiotics overuse. Mupirocin, a clinically used natural antibiotic, inhibits isoleucyl-tRNA synthetase (IleRS), an enzyme links isoleucine its tRNAIle for Two IleRSs, mupirocin-sensitive IleRS1 and resistant IleRS2, coexist in bacteria. The latter may also be found Staphylococcus aureus clinical isolates. Here, we describe the structural...
The rules of the genetic code are established by aminoacyl-tRNA synthetases (aaRSs) enzymes, which covalently link tRNA with cognate amino acid. Many aaRSs involved in diverse cellular processes beyond translation, acting alone, or complex other proteins. However, studies aaRS noncanonical assembly and functions plants scarce, as structural plant aaRSs. Here, we have solved crystal structure Arabidopsis thaliana cytosolic seryl-tRNA synthetase (SerRS), is first crystallographic a aaRS. SerRS...
Abstract To correctly aminoacylate tRNA Leu , leucyl-tRNA synthetase (LeuRS) catalyzes three reactions: activation of leucine by ATP to form leucyl-adenylate (Leu-AMP), transfer this amino acid and post-transfer editing any mischarged product. Although LeuRS has been well characterized biochemically, detailed structural information is currently only available for the latter two stages catalysis. We have solved crystal structures all enzymatic states Neisseria gonorrhoeae during Leu-AMP...
Abstract Antibiotics target key biological processes that include protein synthesis. Bacteria respond by developing resistance, which increases rapidly due to antibiotics overuse. Mupirocin, a clinically used natural antibiotic, inhibits isoleucyl-tRNA synthetase (IleRS), an enzyme links isoleucine its tRNA Ile for Two IleRSs, mupirocin-sensitive IleRS1 and resistant IleRS2, coexist in bacteria. The latter may also be found Staphylococcus aureus clinical isolates. Here, we describe the...
Abstract Isoleucyl-tRNA synthetase (IleRS) is an essential enzyme that covalently couples isoleucine to the corresponding tRNA. Bacterial IleRSs group in two clades, ileS1 and ileS2, latter bringing resistance natural antibiotic mupirocin. Generally, bacteria rely on either or ileS2 as a standalone housekeeping gene. However, we have found exception by noticing Bacillus species with genomic consistently also keep ileS1, which appears mandatory family Bacillaceae . Taking Priestia (Bacillus)...