A5074977316- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Innovation and Socioeconomic Development
- Insect symbiosis and bacterial influences
- Biosensors and Analytical Detection
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Genetics, Aging, and Longevity in Model Organisms
Institute for Integrative Systems Biology
2021-2024
Universitat de València
2021-2024
Viral infections in plants threaten food security. Thus, simple and effective methods for virus detection are required to adopt early measures that can prevent spread. However, current based on the amplification of viral genome by polymerase chain reaction (PCR) require laboratory conditions. Here, we exploited CRISPR-Cas12a CRISPR-Cas13a/d systems detect three RNA viruses, namely, Tobacco mosaic virus, etch Potato X, Nicotiana benthamiana plants. We applied system DNA amplicons generated...
Recurrent disease outbreaks caused by different viruses, including the novel respiratory virus SARS-CoV-2, are challenging our society at a global scale; so versatile detection methods would enable calculated and faster response. Here, we present nucleic acid strategy based on CRISPR-Cas9, whose mode of action relies strand displacement rather than collateral catalysis, using Streptococcus pyogenes Cas9 nuclease. Given preamplification process, suitable molecular beacon interacts with...
Development of a novel framework to regulate translation by repurposing the CRISPR-Cas13 immune system, exploiting cell-free expression system for prototyping.
In public health emergencies or in resource-constrained settings, laboratory-based diagnostic methods, such as RT-qPCR, need to be complemented with accurate, rapid, and accessible approaches increase testing capacity, this will translate into better outcomes disease prevention management. Here, we develop an original nucleic acid detection platform by leveraging the CRISPR-Cas9 lateral flow immunochromatography technologies. combination isothermal amplification that runs a biotinylated...
The novel respiratory virus SARS-CoV-2 is rapidly evolving across the world with potential of increasing its transmission and induced disease. Here, we applied CRISPR-Cas12a system to detect, without need sequencing, genomes harboring E484K mutation, first identified in Beta variant catalogued as an escape mutation. mutation creates a canonical protospacer adjacent motif for Cas12a recognition resulting DNA amplicon, which was exploited obtain differential readout. We analyzed series fecal...
ABSTRACT Recurrent disease outbreaks caused by different viruses, including the novel respiratory virus SARS-CoV-2, are challenging our society at a global scale; so better and handier detection methods would enable faster response. Here, we present nucleic acid strategy based on CRISPR-Cas9, whose mode of action relies strand displacement rather than collateral catalysis, using Streptococcus pyogenes Cas9 nuclease. Given pre-amplification process, suitable molecular beacon interacts with...