An in vivo study of a photoswitchable cytotoxic peptide LMB040 has been undertaken on chemically induced hepatocellular carcinoma model immunocompetent rats. We analysed the pharmacokinetic profile less toxic photoform ("ring-closed" dithienylethene) compound tumors, plasma, and healthy liver. Accordingly, can reach tumor concentration sufficiently high to exert effect upon photoconversion into more active ("ring-open") photoform. Tissue morphology, histology, redox state liver, hepatic...

Quercetin (QUE) and its water-soluble form сorvitin are medicinally important members of the flavonoid family most prominent dietary antioxidants.Numerous pharmacological effects quercetin include protection against diseases, such as atherosclerosis, myocardial infarction, cerebrovascular disea ses.Corvitin modulates liver blood flow but on lipid metabolism have not been understood.We investigated influence corvitin (2.5, 5, 10 mg/kg) formation secretion cholesterol (Chol), esters (EChol),...

One of the synthetic analogues antidiuretic hormone-desmopressin is used in patients with central diabetes insipidus and those coagulation disorders. However, its effects on bile secretion are not fully defined. We investigated effect desmopressin formation determined role V1a vasopressin receptors action choleresis. Rats were injected intraportally a bolus desmopressin; changes flow, content free conjugated cholates compared control animal group. Selective antagonist was 10 minutes before...

Using the transmission electron microscopy (TEM)/high-resolution TEM (HRTEM) and selected area diffraction (SAED) methods, it was shown that nanocolloids of ZnO contain hydrolyzed nanoparticles (NPs). Typically, nanocrystalline ZnO/Zn(OH)2 core is covered by an amorphous shell zinc hydroxides, preventing encapsulated crystal from dissolving. Similar studies were carried out with TiO2 nanocolloids. It found burdening rats for 30 days a aqueous nanocolloid (AN) accompanied narrowing amplitude...

In acute experiments, the effect of Corvitin, water-soluble analogue quercetin, on bile secretion was studied in rats with cannulated duct. Intraportal injections test compound at doses 2.5; 5, and 10 mg/kg resulted a significant increase volume secreted by 20.9, 31.2, 20.4%, respectively, compared control. Mild stimulating effects Corvitin processes acid conjugation taurine glycine, especially when administered dose 5 mg/kg, were shown thin layer chromatography method. The flavonoid did not...

In acute experiments on laboratory rats, intra-portal administration of L-cysteine (20 mg/kg), the precursor hydrogen sulfide synthesis, stimulated oxygen consumption liver by 38.6% and reduced tension 37.1%. Activation tissue respiration occurred due to strengthening oxygen-dependent synthetic processes in liver, particular those associated with mitochondrial enzyme-catalysed bile acid biosynthesis through acidic pathway. The concentrations taurocholic mixtures taurodeoxycholic...

We studied the volume and biochemical components of gastric secretion in pylorus-ligated rats blood flow mucosa exposed to Corvitin doses 2.5 5 mg / kg, intragastrically. Biochemical analysis was based on determining rate hydrochloric acid, concentrations protein, hexosamines (per glucosamine) cysteine. In control rats, hexosamines, mucus indicators, free amino acid cysteine, a powerful antioxidant, have been detected. evoked an increase concentration these compounds juice. However, it did...

The electrical potential of the liver is an integrative indicator activity cells in this organ. Here we tried to elucidate a link between functional state and its activity. For that, liver, content bile acid blood glucose concentration were measured control conditions after intravenous epinephrine administration. Epinephrine at dose 0.2 µg/kg body weight caused short-term increase decrease hydroxylation ratio acids. administration also resulted power spectral density (PSD) electric frequency...

Parkinson’s disease (PD) is symptomatically characterized by motor disorders in the human body, which are associated with degeneration of dopamine neurons substantia nigra midbrain. It widely recognized that mitochondrial dysfunction occurs dopaminergic neurons, leading to their death and development this pathology. Recently, benzodiazepine derivatives have been found a neuroprotective effect against damage an experimental model Рarkinsonian syndrome (PS). The aim study was investigate novel...

П.І. Янчук, С

Essential inter-sexual differences of the lipid metabolism and bile-creation in liver tissue cause necessity substantial experimental studies testosterone influence on bile composition ratio components secretion individuals different sexes. So, aim work was study male female rats. For attaining this aim, (n=7) rats (n=4) were every day during five days intramuscularly administered with propionate (0,7 mg/kg recalculation preparation volume– 1 ml/kg). After that acute experiments vivo, tract...

Досліджено жовчнокислотний склад жовчі самців щурів, які знаходилися в умовах експериментального хронічного соціального стресу.Виявлено, що у стресованих тварин концентрація кон'югованих холатів печінковому секреті значно знижувалась.Так вміст таурохолевої кислоти на 23,6 % був меншим від контролю, а дигідроксихоланових таурокон'югатів (таурохенодезоксихолевої і тауродезоксихолевої кислот) -на 18,9 -23,14 %.Концентрація глікохолевої самців-інтрудерів була 32,9 -51,3 нижчою,...

Among the various functions of liver, formation bile plays an important role. The optimal physiological ratio components and content testosterone in blood depend on factors that can cause biliary system dysfunction secretion. In experiments different-sex rats, changes acid contents under influence propionate, which was injected intramuscularly 0.7 mg/kg, for 5 days were investigated. With method thin-layer chromatography, basic fractions acids conjugated defined – taurocholic,...

The increase of disorders the cholesterol metabolism and bile formation determines need search for possible substances correcting in liver. It is found that, laboratory rats with an experimental drug form steatosis, excretion and, especially, esters to ducts inhibited, their ratio significantly impaired. use bioadditive “FLP-MD” made on milk phospholipids has a corrective effect concentration its tetracycline-induced steatosis. This allows us recommend improve patients clinical cases...

More than 90 % of uncomplicated upper respiratory tract infections are caused by viruses, with a risk developing subsequent bacterial in some patients. The development microbial complications depends on the degree viral damage structure mucosa and its functions. objective this work was to study effect Sinupret oral drops nasal atrophic changes rat model experimental rhinitis. 60 female rats Wistar were allocated for study. To phytopreparation atrophy epithelium animals divided into 3 groups....

Influence of calcitonin on the choleresis level and bile acids spectrum was investigated in acute experiments rats with common biliary duct cannulated. We determined intensity secretion during 3 hours quantitative content conjugated free using thin-layer chromatography. It shown that synthetic salmon at intramuscular introduction (200 ng/kg) increases secreted volume bile. The administration resulted increase (+17.4% (Р < 0.05)), taurotrihydroxycholates (+58.3% (P 0.001)),...

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the loss of dopaminergic neurons in substantia nigra. The cause PD not fully understood, and effective treatments still do exist. It believed that oxidative stress, mitochondrial dysfunction, impaired lipid metabolism may underlie pathogenesis PD. Bile contains breakdown products various compounds form hepatocytes. This study aimed to evaluate effect new benzodiazepine derivative - diazepinone (DP) on purine liver rats...

Aim. To estimate the impact of low temperatures on lipid composition reproductive cells male sterlets (Acipenser ruthenus L) in cryopreservation environment. Methods. The determination sperm quality (color, consistence, concentration and motility spermatozoa) was estimated by common biochemical methods using standard equipment. Thin-layer chromatography used to reveal fi ve fractions neutral lipids investigated sh, which were distributed plates as follows: starting line demonstrated...

The effects of flutamide (25 mg/kg, intragastrically, daily for three days) on bile formation in male rats were investigated vivo experiments, and the acid composition was analyzed. volume rate secretion decreased under influence flutamide, as well concentration glycoconjugated acids action. In particular, biliary glycochenodeoxycholate glycodeoxycholate concentrations are significantly lower (by 16.3-34.5%) action, compared to those samples control group animals. this situation, fails...

Parkinson's disease (PD) is a progressive neurodegenerative disorder, characterized by the loss of dopaminergic neurons in substantia nigra pars compacta.The causes PD are not fully understood; however, increasing evidence implicates disturbed respiratory function mitochondria and lack energy cells.Aim.To study effects 2.3-diazepine (2.3-DP), new derivative benzodiazepine, on liver tissue respiration (LTR) dependent processes bile acids (BAs) production rat model PD.Methods.PD was induced...

This study aims to evaluate the effect of methanindiazenone (МD), a new benzodiazepine derivative, on levels purine metabolites and lipids in blood plasma rats with rotenone (ROT) induced Parkinson’s disease (PD). The concentrations ATP, ADP, AMP, xanthine, hypoxanthine, phospholipids (PL), cholesterol (CHOL), esters (ECHOL), free fatty acids (FFA), triglycerides (TG) were quantified samples by thin-layer chromatography. Our data demonstrate that ROT-induced PD AMP/ATP ratio increased 2.5...

In acute experiments on anesthetized rats, L-arginine has been shown to enhance oxygen-dependent synthetic processes in the liver, particularly those associated with mitochondrial polyenzyme systems of bile acid biosynthesis, simultaneous activation hepatocyte tissue respiration. At that, level oxygen tension gland liver remains unchanged. Nitric oxide contributes only partly implementation these effects. Moreover, inhibits cholepoiesis choleresis without involving NO reaction....