A5048036321- bioluminescence and chemiluminescence research
- Pharmaceutical and Antibiotic Environmental Impacts
- Phytochemicals and Antioxidant Activities
- Biosensors and Analytical Detection
- Free Radicals and Antioxidants
- Advanced biosensing and bioanalysis techniques
- Gene Regulatory Network Analysis
- Bioactive Compounds and Antitumor Agents
- Computational Drug Discovery Methods
- Agriculture, Plant Science, Crop Management
- Chemistry and Chemical Engineering
- Monoclonal and Polyclonal Antibodies Research
- Selenium in Biological Systems
- Advanced Chemical Sensor Technologies
- thermodynamics and calorimetric analyses
- Viral Infectious Diseases and Gene Expression in Insects
- Chemical Reactions and Isotopes
- Analytical chemistry methods development
- Essential Oils and Antimicrobial Activity
- Environmental Toxicology and Ecotoxicology
- CRISPR and Genetic Engineering
- Water Quality Monitoring and Analysis
- Cell Image Analysis Techniques
- Inorganic and Organometallic Chemistry
- Advanced Biosensing Techniques and Applications
Bialystok University of Technology
2015-2024
University of Białystok
2011
Vanillic acid (4-hydroxy-3-methoxybenzoic acid) (VA) is a natural benzoic derivative commonly found in herbs, rice, maize, and some fruits vegetables. However, due to the wide use of VA various industrial sectors, its presence environment might harm living organisms. This study evaluated toxicity isomers, iso-VA orto-VA. Firstly, antimicrobial effect isomers orto-VA (in doses 1000; 100, 10, 1; 0.1; 0.01 mg/L) against Escherichia coli, Sarcina spp., Enterobacter homaechei, Staphylococcus...
The Na(I), Zn(II) and Ni(II) complexes with quercetin have been synthesized. composition of these compounds was established by means elemental thermogravimetric analyses. To study the molecular structure synthesized compounds, many miscellaneous analytical methods, which complement one another, were used: infrared (FT-IR), Raman (FT-Raman) electronic absorption UV/VIS spectroscopy. For studying cytotoxic genotoxic activity bioreporter strain Escherichia coli K-12 recA::gfpmut2 has used.
N6-benzyladenine and kinetin are adenine-type cytokinins that play various roles in many aspects of plant development stimulate anabolic processes cells. The aim this study was to examine the effect on basic oxidative stress parameters, such as antioxidative enzyme activity, reduced glutathione thiol group content, lipid peroxidation. results show a stimulatory well content. Cytokinins caused decrease membrane phospholipid peroxidation exhibited protective properties against malondialdehyde...
Lithium, sodium, potassium, rubidium and caesium salts of 5-O-caffeoylquinic acid (chlorogenic acid, 5-CQA) were synthesized described by FT-IR (infrared spectroscopy), FT-Raman (Raman UV (UV absorption ¹H (400.15 MHz), 13C (100.63 MHz) NMR (nuclear magnetic resonance spectroscopy). The quantum-chemical calculations at the B3LYP/6-311++G** level done in order to obtain optimal structures, IR spectra, NBO (natural bond orbital) atomic charges, HOMO (highest occupied molecular LUMO (lowest...
The following article discusses the antioxidant properties of mandelic acid and its hydroxy methoxy derivatives. capacity these compounds is determined by DPPH, FRAP, CUPRAC ABTS. mechanisms underlying are described BDE, IP, PDE, ETE PA calculation method values referenced to experimental data. Thermochemistry, HOMO/LUMO energies, dipole moments, charge distribution, IR, RAMAN, NMR frequencies, binding lengths angles were calculated using B3LYP 6-311++G(d,p) basis set. structure derivatives...
This study concerns on the influence of diamines (agmatine, putrescine) and polyamines (spermine, spermidine) upon growth content chlorophyll <em>a</em> <em>b</em>, monosaccharides proteins in cells alga <em>Chlorella vulgaris </em>Beijerinck (Chlorophyceae). In experiments agmatine, putrescine, spermine spermidine range concentrations 10<sup>-6</sup>-10<sup>-3</sup> M were used. At concentration 10<sup>-3</sup> 1st day...
Dacarbazine (DAC) 5-(3,3-dimethyl-1-triazenyl)imidazole-4-carboxamide is an imidazole-carboxamide derivative that structurally related to purines. DAC belongs the triazene compounds, which are a group of alkylating agents with antitumor and mutagenic properties. non-cell cycle specific drug, active in all phases cellular cycle. In frame this work 3d metal complexes (cobalt copper) dacarbazine were synthesized. Their spectroscopic properties by use FT-IR, FT-Raman, 1HNMR studied. The...
In the present work, conducted research concerned determination of toxicity and oxidative stress generation antidepressant fluoxetine (FLU), its metabolite nor-fluoxetine (Nor-FLU), antibiotic nalidixic acid (NA), caffeic (CA) their mixtures in three different environments: microbial medium (MM), raw wastewaters (RW) treated (TW). We evaluated following parameters: E. coli cell viability, protein damage, sodA promoter induction ROS generation. It was found that FLU, Nor-FLU, NA, CA are toxic...
In environmental matrices there are mixtures of parent drug and its metabolites.The majority research is focused on the biological activity toxic effect diclofenac (DCF), little DCF metabolites their mixtures.The study assessment impact DCF, 4'-hydroxydiclofenac (4'-OHDCF) 5-hydroxydiclofenac (5-OHDCF) E. coli strains.The effects tested chemicals were evaluated using following: K-12 cells viability assay, inhibition bacteria culture growth, ROS (reactive oxygene species) generation...
Escherichia coli K-12 GFP-based bacterial biosensors allowed the detection of cytotoxic and genotoxic effect anticancer drug-cyclophosphamide antidiabetic drug -metformin in PBS buffer surface water.Experimental data indicated that recA::gfpmut2 genetic system was sensitive to drugs mixture applied experiment.RecA promoter a good bioindicator screening cyclophosphamide, metformin both water.The results E. recA::gfp mut2 strain could be potentially useful for first-step pharmacist residues...
The autofluorescent reporter of Escherichia coli K-12 recA::gfpmut2 strain containing a plasmid-borne transcriptional fusion between DNA-damage genotoxin-inducible recA promoter involved in the SOS regulon response and fast folding GFP (green fluorescent protein) variant gene-gfpmut2, have been used. GFP-based bacterial biosensors allowed for detection cytotoxic genotoxic anticancer drug—a cisplatin antidiabetic drug—metformin PBS buffer surface water. Experimental data indicated that...
: Dacarbazine, DAC, 5-(3,3-dimethyltriazeno)imidazol-4-carboxamideis is an imidazole-carboxamide derivative, that structurally related to purines. DAC belongs the triazene compounds, which are a group of alkylating agents with antitumour and mutagenic properties. non-cell cycle specific drug, active on all phases cellular cycle. In frame this work 3d-metal complexes (cobalt copper) dacarbazine were synthesized. Their spectroscopic properties by use FT-IR, FT-Raman 1HNMR studied. The...
The research used bacterial biosensors containing luciferase genes to monitor changes in the environment real-time. In this work express four different gene constructs: recA:luxCDABE, soxS:luxCDABE, micF:luxCDABE, and rpoB:luxCDABE Escherichia coli SM lux biosensor after exposure three antibiotics (nalidixic acid, ampicillin, kanamycin) diclofenac was determined. It found that incubation of E. strain various concentrations analytes results differentiation expression at each tested (from...