A5023135639- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Nerve injury and regeneration
- Neuroscience and Neuropharmacology Research
- Tendon Structure and Treatment
- Spinal Cord Injury Research
- Neurogenesis and neuroplasticity mechanisms
- Cell death mechanisms and regulation
- Genetic Neurodegenerative Diseases
- Herpesvirus Infections and Treatments
- Nuclear Receptors and Signaling
- Retinal Development and Disorders
- Lipid Membrane Structure and Behavior
- Pharmacological Receptor Mechanisms and Effects
- Viral Infections and Immunology Research
- Receptor Mechanisms and Signaling
- RNA regulation and disease
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Neurological disorders and treatments
- Genetics and Neurodevelopmental Disorders
University of Auckland
2001-2020
Adeno-associated viral (AAV) vectors have become the primary delivery agent for somatic gene transfer into central nervous system (CNS). To date, AAV-mediated to CNS is based on serotypes 1-9, with efficient neurons only-selective and widespread transduction of glial cells not been observed. Recently, additional endogenous AAVs isolated from nonhuman primate tissues. In this study, obtained AAV bb2, cy5, rh20, rh39, rh43 was compared that AAV8, another isolate previously shown perform well...
The atypical antipsychotic olanzapine is a potent activator of mutated muscarinic receptor used for chemogenetic inhibition.
Adeno-associated viral vectors (AAV) have been shown to be safe in the treatment of retinal degenerations clinical trials. Thus, improving efficiency gene delivery has become increasingly important increase success In this study, structural domains different rAAV serotypes isolated from primate brain were combined create novel hybrid recombinant AAV serotypes, rAAV2/rec2 and rAAV2/rec3. The efficacy these assessed wild type mice two models degeneration (the Abca4−/− mouse which is a model...
cAMP response element-binding protein (CREB) is important for the formation and facilitation of long-term memory in diverse models. However, to our knowledge, involvement CREB age-associated impairment has not been reported. Here, we use a recombinant adeno-associated virus vector obtain stable transgenic expression as well inducible early repressor (ICER) hippocampus adult rats. In longitudinal study, show that somatic gene transfer both ICER does alter young (3-month-old) at 15 months age,...
Aldehyde dehydrogenase family 1, member L1 (ALDH1L1) is a recently characterized pan-astrocytic marker that more homogenously expressed throughout the brain than classic astrocytic marker, glial fibrillary acidic protein. We generated putative promoter sequence variants of rat ALDH1L1 gene for use in adeno-associated viral vector-mediated transfer, with an aim to achieve selective regulation transgene expression astrocytes brain. Unexpectedly, mediated transcriptional activity exclusively...
Huntington's disease (HD) is caused by a dominant mutation that results in an unstable expansion of CAG repeat the huntingtin gene leading to toxic gain function protein which causes massive neurodegeneration mainly striatum and clinical symptoms associated with disease. Since has multiple effects cell precise mechanism remains be elucidated, therapy approaches have been developed intervene different aspects condition. These include increasing expression growth factors, decreasing levels...
Abstract Chondroitin sulphate proteoglycans (CSPGs) are inhibitors to axon regeneration and plasticity. Bacterial chondroitinase ABC degrades CSPGs has been extensively reported be therapeutic after SCI but there remain concerns for its clinical translation. A disintegrin metalloproteinase with thrombospondin motifs-4 (ADAMTS4) is a human enzyme that catalyses the proteolysis of CSPG protein cores. Infusion ADAMTS4 into damaged spinal cord was previously shown improve functional recovery...