A5084048144- Bioinformatics and Genomic Networks
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Computational Drug Discovery Methods
- Marine and coastal ecosystems
- 14-3-3 protein interactions
- Monoclonal and Polyclonal Antibodies Research
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- CRISPR and Genetic Engineering
- Metalloenzymes and iron-sulfur proteins
- Aquatic Ecosystems and Phytoplankton Dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- Immune cells in cancer
- Protein Degradation and Inhibitors
- Immune Cell Function and Interaction
- Microbial Community Ecology and Physiology
- Advanced biosensing and bioanalysis techniques
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- Biocrusts and Microbial Ecology
- Biosimilars and Bioanalytical Methods
- Amino Acid Enzymes and Metabolism
- Biofuel production and bioconversion
- Cancer Immunotherapy and Biomarkers
University of Oxford
2024-2025
Colorado School of Mines
2020-2024
National Renewable Energy Laboratory
2024
National Institutes of Health
2024
National Cancer Institute
2024
Institute of Bioinformatics
2010-2023
Savitribai Phule Pune University
2023
University of Alberta
2018-2020
Rutgers Sexual and Reproductive Health and Rights
2013-2019
Rutgers, The State University of New Jersey
2011-2019
With fast growth rates, broad halotolerance and the ability to thrive at high temperatures, algae in genus Picochlorum are emerging as promising biomass producers. Recently, we isolated a remarkably productive strain, celeri, that attains > 40 g m
Upon nutrient deprivation, microalgae partition photosynthate into starch and lipids at the expense of protein synthesis growth. We investigated role biosynthesis with respect to photosynthetic growth carbon partitioning in Chlamydomonas reinhardtii starchless mutant, sta6, which lacks ADP-glucose pyrophosphorylase. This mutant is unable convert glucose-1-phosphate ADP-glucose, precursor biosynthesis. During nutrient-replete culturing, sta6 does not re-direct metabolism make more proteins or...
Abstract Modulation of photoassimilate export from the chloroplast is essential for controlling distribution fixed carbon in cell and maintaining optimum photosynthetic rates. In this study, we identified TRIOSE PHOSPHATE/PHOSPHATE TRANSLOCATOR 2 (CreTPT2) CreTPT3 green alga Chlamydomonas (Chlamydomonas reinhardtii), which exhibit similar substrate specificities but whose encoding genes are differentially expressed over diurnal cycle. We focused mostly on because its high level expression...
Abstract Loss of IFNγ-sensitivity by tumours is thought to be a mechanism enabling evasion, but recent studies suggest that IFNγ-resistant can sensitised for immunotherapy, yet the underlying remains unclear. Here, we show IFNγ receptor-deficient B16-F10 mouse melanoma are controlled as efficiently WT despite their lower MHC class I expression. Mechanistically, receptor deletion in increases availability, triggering remodelling immune landscape characterised inflammatory monocyte...
Abstract Multiple, dissimilar genetic defects in cancers of the same origin contribute to heterogeneity tumor phenotypes and therapeutic responses patients, yet associated molecular mechanisms remain elusive. Here, we show at systems level that serous ovarian carcinoma is marked by activation interconnected modules with a specific gene set was derived from three independent tumor-specific expression data sets. Network prediction algorithms combined preestablished protein interaction networks...
Domestication of algae for food and renewable biofuels remains limited by the low photosynthetic efficiencies processes that have evolved to be competitive optimal light capture, incentivizing development large antennas in light-limiting conditions, thus decreasing efficient utilization cultivated ponds or photobioreactors. Reducing pigment content improve biomass productivity has been a strategy discussed several decades ability reduce significantly is now fully at hand thanks widespread...
Microalgae are compelling renewable resources with applications including biofuels, bioplastics, nutrient supplements, and cosmetic products. Picochlorum celeri is an alga high industrial interest due to exemplary outdoor areal biomass productivities in seawater. Detailed proximate analysis needed multiple environmental conditions understand the dynamic compositions of P. celeri, how these might be leveraged biotechnological applications. In this study, characterization was performed under...
High cellular pigment levels in dense microalgal cultures contribute to excess light absorption. To improve photosynthetic yields the marine microalga
Metabolic engineering of cyanobacteria with concomitant electrochemical elimination H<sub>2</sub>uptake increases H<sub>2</sub>yield beyond the Thauer limit.
CcmR, a LysR-type transcriptional regulator, represses the genes encoding components of high-affinity carbon concentration mechanism in cyanobacteria. Unexpectedly, deletion ccmR gene was found to alter expression terminal oxidase and fermentative genes, especially hydrogenase operon cyanobacterium Synechococcus sp. PCC 7002. Consistent with transcriptomic data, strain exhibits flux increases (30-50%) both aerobic O2 respiration anaerobic H2 evolution. To understand how CcmR influences...
Synechococcus sp. PCC 7002 is a unicellular cyanobacterium capable of fast growth and tolerance to high light intensity salinity. These attributes along with genetic tractability make an attractive candidate for industrial scale production specialty commodity chemicals. LS (Davies et al., Front Bioeng Biotechnol, 2014, 2, 21–11) produces limonene, energy dense diesel jet fuel drop-in additive, at titer 4 mg/L over 4-day incubation period. In this study, we use the state-of-the-art whole-cell...
Immune responses against cancer are inherently stochastic, with small numbers of individual T cells within a larger ensemble lymphocytes initiating the molecular cascades that lead to tumor cytotoxicity. A potential source this intra-tumor variability is differential ability immune respond cells. Classical microwell co-cultures and inadequate for reliably culturing analyzing low cell needed probe variability, have failed in recapitulating heterogeneous domains observed tumors. Here we...
Abstract Loss of IFNγ-sensitivity by tumours is thought to be a mechanism enabling evasion, as some cancers lacking IFNγ-signalling demonstrate resistance checkpoint immunotherapy. However, recent studies demonstrated that IFNγ-resistant are well-controlled and sensitized for The underlying leading enhanced immune responses in those patients unknown. Using IFNγ-insensitive melanoma which were the endogenous anti-tumour response, we found despite low basal MHC class I expression tumours, CD8...
<div>Abstract<p>Multiple, dissimilar genetic defects in cancers of the same origin contribute to heterogeneity tumor phenotypes and therapeutic responses patients, yet associated molecular mechanisms remain elusive. Here, we show at systems level that serous ovarian carcinoma is marked by activation interconnected modules with a specific gene set was derived from three independent tumor-specific expression data sets. Network prediction algorithms combined preestablished protein...
Details of A4 Cell System from Gene Expression: Protein Interaction Systems Network Modeling Identifies Transformation-Associated Molecules and Pathways in Ovarian Cancer