A5009685633- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- CRISPR and Genetic Engineering
- Enzyme Catalysis and Immobilization
- Carbon and Quantum Dots Applications
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Electrochemical sensors and biosensors
- Clostridium difficile and Clostridium perfringens research
- Enzyme Production and Characterization
- Advanced biosensing and bioanalysis techniques
- Enzyme-mediated dye degradation
- Anaerobic Digestion and Biogas Production
- Enzyme Structure and Function
- Pharmacogenetics and Drug Metabolism
- biodegradable polymer synthesis and properties
- Reproductive biology and impacts on aquatic species
- Nanomaterials for catalytic reactions
- 3D Printing in Biomedical Research
- Steroid Chemistry and Biochemistry
- Selenium in Biological Systems
- Bacterial biofilms and quorum sensing
- Transgenic Plants and Applications
- Protein Interaction Studies and Fluorescence Analysis
Zhengzhou University
2020-2024
Chinese Academy of Sciences
2014-2020
Center for Excellence in Molecular Plant Sciences
2014-2020
Shanghai Institutes for Biological Sciences
2014-2016
University of Chinese Academy of Sciences
2014-2016
Abstract Solventogenic clostridia are important industrial microorganisms that produce various chemicals and fuels. Effective genetic tools would facilitate physiological studies aimed both at improving our understanding of metabolism optimizing solvent productivity through metabolic engineering. Here we have developed an all‐in‐one, CRISPR‐based genome editing plasmid, pNICKclos, can be used to achieve successive rounds gene in Clostridium acetobutylicum ATCC 824 beijerinckii NCIMB 8052...
Genetic modifications of bacterial chromosomes are important for both fundamental and applied research. In this study, we developed an efficient, easy-to-use system genetic modification the Escherichia coli chromosome, a two-plasmid method involving lambda Red (λ-Red) recombination I-SceI cleavage. An intermediate strain is generated by integration resistance marker gene(s) recognition sites in or near target gene locus, using λ-Red PCR targeting. The transformed with donor plasmid carrying...
In China, aquaculture costs have increased because of the increase in fish meal (FM) prices. Plant proteins, such as soybean meal, potential to replace FM partially and thus reduce cost aquatic feed. this study, (SBM) was fermented using compound microorganisms (lactic acid bacteria, yeast, Bacillus). Fermented (FSBM) replaced diet. The effect replacing on largemouth bass comprehensively evaluated at three levels: macro (growth performance), microbial (bacterial diversity metabolic), gene...
Organic selenium nanoparticles (SeNPs) are attracting great interest due to their unique properties and potential applications in the food industry. In this study, a novel form of SeNPs was synthesized by using Bacillus licheniformis CGMCC 2876 extracellular polymeric substances (EPS) fermented from waste cane molasses. The morphology EPS-SeNPs spherical with an average size 141.8 nm zeta −51.77 mV, concentration Se reached 107.43 mg/g. Additionally, exhibited good stability aqueous solution...
Increasing demand for recombinant proteins necessitates efficient protein production processes. In this study, a continuous process human epidermal growth factor (hEGF) secretion by
The production of acetone–butanol–ethanol by solventogenic Clostridium using lignocellulosic biomass can be a potential alternative to petroleum-based butanol. However, previous studies on nondetoxified lignocellulose hydrolysate could not provide better results when compared those in synthetic medium. In this study, we engineered the pentose pathway beijerinckii NCIMB 8052, which was then subjected adaptive laboratory evolution gradient mixture medium and pretreated corn stover enzymatic...
Biofilms can enhance industrial fermentation efficiency by increasing cell density, stability, and metabolic activity have been successfully applied to the continuous production of many small-molecule chemicals. However, proteins biofilms has less studied. This study used secretory human epidermal growth factor (hEGF) as a representative product evaluate optimize biofilm-based protein production. First, deleting protease overexpressing eight key genes involved in secretion Saccharomyces...
During the production of ethanol from lignocellulose-derived sugars, recombinant yeasts tend to utilize xylose and arabinose after glucose exhaustion. So far, many glucose-insensitive pentose transporters have been reported counteract this phenomenon, but few studies described intracellular factors. In study, combination adaptive evolution, comparative genomics, genetic complementation revealed that hexokinase-deficient (Hxk0) arabinose-fermenting Saccharomyces cerevisiae requires...
Biofilm-mediated continuous fermentation with cells immobilized has gained much attention in recent years. In this study, thermoresponsive poly(N-isopropylacrylamide)-grafted cotton fibers (PNIPAM-CF) were prepared via an improved surface-initiated atom transfer radical polymerization. The modification process imparted switchable wettability to the surface while maintaining thermal stability and biocompatibility of CF. During ethanol transformation, rapid, reversible cell adsorption...
A novel magnetic 2D/2D heterogeneous structure MXene@NiFe-LDH@Fe3O4 was prepared for immobilization of laccase. In this work, two-dimensional MXene nanosheets with abundant surface functional groups were heterogeneously assembled layered double hydroxide (LDH) by in situ co-precipitation method, and nanoparticle Fe3O4 excellent biocompatibility rapid separation materials substrates introduced subsequently, then silane coupling agent coated on the . The functionalized employed as a carrier to...