A5080853712- Barrier Structure and Function Studies
- Neonatal and fetal brain pathology
- Drug Transport and Resistance Mechanisms
- Pharmacological Effects and Toxicity Studies
- Pregnancy and Medication Impact
- Pregnancy and preeclampsia studies
- Spinal Cord Injury Research
- Metabolism and Genetic Disorders
- Trace Elements in Health
- Neuroscience and Neuropharmacology Research
- Folate and B Vitamins Research
- Neurogenesis and neuroplasticity mechanisms
- Erythrocyte Function and Pathophysiology
- Nerve injury and regeneration
- Iron Metabolism and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Hemoglobinopathies and Related Disorders
- Cerebrospinal fluid and hydrocephalus
- Neonatal Health and Biochemistry
- Prenatal Substance Exposure Effects
- Bone and Dental Protein Studies
- Ion Transport and Channel Regulation
- Fetal and Pediatric Neurological Disorders
- Birth, Development, and Health
- Connexins and lens biology
Australian Regenerative Medicine Institute
2022-2025
Monash University
2021-2025
The University of Melbourne
2014-2023
The Alfred Hospital
2022
University of Copenhagen
1981-2018
Advisory Board Company (United States)
2008
Madison Group (United States)
2008
RELX Group (United Kingdom)
2008
Singer (United States)
2008
University of Tasmania
1992-2002
The adult brain functions within a well-controlled stable environment, the properties of which are determined by cellular exchange mechanisms superimposed on diffusion restraint provided tight junctions at interfaces between blood, and cerebrospinal fluid (CSF). These referred to as "the" blood-brain barrier. It is widely believed that in embryos newborns, this barrier immature or "leaky", rendering developing more vulnerable drugs toxins entering fetal circulation from mother. New evidence...
1. The development of the blood‐brain and blood‐c.s.f barriers to lipid insoluble substances different molecular radii has been studied in fetal sheep, early (60 days) late (125 gestation, using labelled erythritol (C14), sucrose (3H or 14C), inulin 14C) albumin (125I), IgG detected by immunoassay. 2. Morphological studies brain choroid plexus at same gestational stages were carried out thin section electron microscopy freeze fracture techniques. 3. Penetration markers into c.s.f. was...
Barrier mechanisms in the brain are important for its normal functioning and development. Stability of brain's internal environment, particularly with respect to ionic composition, is a prerequisite fundamental basis function, namely transmission nerve impulses. In addition, appropriate controlled supply wide range nutrients such as glucose, amino acids, monocarboxylates, vitamins also essential development function. These all cellular functions across interfaces that separate from rest...
In the adult interface between cerebrospinal fluid and brain is lined by ependymal cells, which are joined gap junctions. These intercellular connections do not provide a diffusional restrain two compartments. However, during development this interface, initially consisting of neuroepithelial cells later radial glial characterized "strap" junctions, limit exchange different sized molecules parenchyma. Here we systematic study permeability properties inner fluid-brain barrier mouse from...
We have evaluated a small water-soluble molecule, biotin ethylenediamine (BED, 286 Da), as permeability tracer across the blood-brain barrier. This molecule was found to suitable characteristics in that it is stable plasma, has low plasma protein binding, and appears behave similar manner brain barriers established by markers such sucrose. BED, together with 3000-Da biotin-dextran (BDA3000), used investigate effectiveness of tight junctions cortical vessels during development adulthood...
Abstract Epidemiological evidence in human fetuses links inflammation during development with white matter damage. Breakdown of the blood–brain barrier has been proposed as a possible mechanism. This was investigated present study by inducing prolonged inflammatory response newborn rats, intraperitoneal injections lipopolysaccharide (LPS; 0.2 mg/kg) given at postnatal (P) day 0, P2, P4, P6 and P8. An acute phase over whole period injections. Changes permeability were determined for small...
The cDNA sequence encoding the bovine fetal protein fetuin is reported. deduced amino acid identical with that obtained from sequencing. a single chain preceded by signal sequence. three N-linked glycosylation sites have been determined. of shows over 70% similarity to human alpha 2HS glycoprotein. All cysteine residues are conserved in both proteins, suggesting has same arrangement disulfide loops as glycoprotein and may also be member cystatin family. Southern blot analysis indicates gene...
1. The concentration of total protein in c.s.f. and plasma has been measured fetal sheep different gestational ages the adult. In it was highest (approximately 840 mg/100 ml.) youngest fetuses (35 days) declined steeply by 60 days (260 ml.). It decreased less markedly last half gestation to reach about 50 ml. at 125 which is twice adult value. Protein lowest did not rise much until second during time doubled. There a further rather larger increase between late (125 stage 2. Albumin, fetuin,...
Cellular mechanisms of secondary damage progression following spinal cord injury remain unclear. We have studied the extent tissue from 15 min to 10 weeks after using morphological and biochemical estimates lesion volume surviving grey white matter. This has been achieved by semi-quantitative immunocytochemical methods for a range cellular markers, quantitative counts matter axonal profiles in semi-thin sections Western blot analysis, together with behavioural tests (BBB scores, ledged beam,...
1. The penetration of human and sheep plasma proteins from blood into c.s.f. fetuses (57‐86 days gestation) has been studied. were injected intravenously via cotyledonary vessels. After different time periods the concentrations marker estimated by radioactive counting iodinated or immunoassay proteins. 2. Several similar molecular size penetrated to a extent in 60 day fetuses. steady‐state c.s.f.: ratios about 15% for AFP, 10% transferrin albumin, 7% α 1 ‐antitrypsin, 5% albumin. In older...
We provide comprehensive identification of embryonic (E15) and adult rat lateral ventricular choroid plexus transcriptome, with focus on junction-associated proteins, ionic influx transporters channels. Additionally, these data are related to new structural previously published permeability studies. Results reveal that most genes associated intercellular junctions expressed at similar levels both ages. In total, 32 molecules known be brain barrier interfaces were identified. Nine claudins...