The objective was to investigate the effect of kinsenoside (Kin) treatments on macrophage polarity and evaluate resulting protection chondrocytes attenuate osteoarthritis (OA) progression. RAW264.7 macrophages were polarized M1/M2 subtypes then administered with different concentrations Kin. polarization transitions evaluated quantitative real-time polymerase chain reaction (qRT-PCR), confocal observation flow cytometry analysis. mechanism Kin repolarizing M1 by Western blot. Further,...

Staphylococcus aureus ( S. aureus) is a causative agent in life-threatening human diseases that afflict millions of people annually. Traditional antibiotic treatments are becoming less efficient because can invade host cells including osteoblasts and macrophages, constituting reservoir relatively protected from antibiotics lead to recrudescent infection. We herein report unique intracellular delivery nanoparticle, which composed (i) mesoporous silica nanoparticle (MSN) core loaded with...

Biofilms, which are complex microbial communities embedded in the protective extracellular polymeric substances (EPS), difficult to remove food production facilities. In this study, use of acidic electrolyzed water (AEW) foodborne pathogen biofilms was evaluated. We used a green fluorescent protein-tagged Escherichia coli for monitoring efficiency AEW removing biofilms, where under optimal treatment conditions, signal cells biofilm disappeared rapidly and population reduced by more than 67%....

The early detection and thus treatment of breast cancer bone metastasis remain a big challenge clinically. As the most abundant cells within tissue, osteocytes have been found to manipulate activity by its crosstalk with osteoclasts. However, conventional bone-targeting nanomedicine has limited bone-lesion specificity ignores vital role during metastasis. Also, it lacks detailed insight into therapeutic mechanisms, which hinders following translational practice. Previously, we shown that...

Accumulating evidence suggests that activation of proinflammatory M1-type macrophages in the synovium plays a vital role progression osteoarthritis (OA). Redundant nitric oxide (NO) and hydrogen peroxide (H2O2) are key factors drive to polarize M1 type. Herein, modified zeolitic imidazolate framework-8 (ZIF-8) nanoparticles (NPs) have been synthesized. By regulating intracellular gases reprogramming metabolism phenotype, NPs transformed macrophage polarization from anti-inflammatory M2...

Zinc-based biomaterials, including biodegradable metal, nanoparticles, and coatings used in medical implants release zinc ions that may increase the whole-body serum concentrations. The impact of concentrations on major health outcomes can provide insights for device design clinical transformation zinc-based biomaterials.This nationally representative cross-sectional study enrolled participants from National Health Nutrition Examination Survey (NHANES, 2011-2014) 3607 participants. Using...

Both copper deficiency and overexposure have been associated with adverse health effects. Evidence linking to bone mineral density (BMD) total fracture, however, is limited.This nationally representative cross-sectional study enrolled participants from the National Health Nutrition Examination Survey (2011-2014) in United States. Using unadjusted multivariate adjusted logistic regression analyses a two-piecewise linear model smoothing function, we evaluated associations between serum levels,...

Abstract The loss of appropriate cell adhesion normally induces apoptosis via a process termed anoikis. aim this study was to investigate the effects mesenchymal stem cells (MSCs) in cancer microenvironment on anoikis resistance and pulmonary metastasis osteosarcoma (OS) cells, evaluate critical role interleukin (IL)-8/C-X-C chemokine receptor (CXCR) 1/Akt-signaling pathway these processes. Metastatic OS subtype which did or not interact with MSC-conditioned medium (MSC-CM) vitro, were...

Abstract Fleshy fruit ripening is typically regulated by ethylene in climacteric fruits and abscisic acid (ABA) non‐climacteric fruits. Common fig ( Ficus carica ) shows a dual‐ripening mechanism, which not fully understood. Here, we detected separate peaks of ABA at the onset‐ on‐ripening stages, conjunction with sharp rise glucose fructose contents. In newly‐designed split‐fruit system, exogenous failed to rescue fluridone‐inhibited ripening, whereas rescued 2‐amino‐ethoxy‐vinyl glycine...

Abstract Increasing evidence indicates that osteoarthritis (OA) is a musculoskeletal disease affecting the whole joint, including both cartilage and subchondral bone. Reactive oxygen species (ROS) have been demonstrated to be one of important destructive factors during early‐stage OA development. The objective this study was investigate isorhamnetin (Iso) treatment on osteoclast formation chondrocyte protection attenuate by modulating ROS. Receptor activator nuclear factor‐kappa B ligand...

Background: Cerium oxide nanoparticles (CeO 2 NPs) are potent scavengers of cellular reactive oxygen species (ROS). Their antioxidant properties make CeO NPs promising therapeutic agents for bone diseases and tissue engineering. However, the effects on intracellular ROS production in osteoclasts (OCs) still unclear. Numerous studies have reported that essential osteoclastogenesis. The aim this study was to explore osteoclast differentiation potential underlying mechanisms. Methods:...

The complex three-dimensional structure of biofilms is supported by extracellular polymeric substances (EPSs) and additional insight on chemical variations in EPS biofilm development will inform strategies for control biofilms. Vibrio parahaemolyticus VPS36 was studied using confocal laser scanning microscopy (CLSM) Raman spectroscopy (RM). structural parameters the (biovolume, mean thickness, porosity) were characterized CLSM results showed that formed dense structures after 48 h...

Abstract Gouty arthritis is a rheumatic disease that characterized by the deposition of monosodium urate (MSU) in synovial joints cause increased serum hyperuricemia. This study used three-dimensional (3D) flowing microfluidic chip to screen effective candidate against MSU-stimulated human umbilical vein endothelial cell (HUVEC) damage, and found kinsenoside (Kin) be leading active component Anoectochilus roxburghi , one Chinese medicinal plant widely treatment gouty clinically. Cell...

Abstract The treatment of breast cancer-induced osteolysis remains a challenge in clinical settings. Here, we explored the effect and mechanism combined with zoledronic acid (ZA) plumbagin (PL), widely investigated component derived from Plumbago zeylanica , against osteoclastogenesis. We found that PL ZA suppressed cell viability precursor osteoclasts synergistically inhibited MDA-MB-231-induced osteoclast formation (combination index=0.28) abrogation recombinant mouse receptor activator...

Abstract The endocrine role of the skeleton-which is impaired in human diseases including osteoporosis, obesity and diabetes-has been highlighted previously. In these diseases, AMPK, a sensor regulator energy metabolism, biological clinical importance. Since AMPK’s main catalytic subunit α has two isoforms, it unclear whether functional differences between them exist skeletal system. current study overexpressed AMPKα1 α2 MC3T3-E1 cells, primary osteoblasts mouse BMSCs by lentiviral...

In hypoxic dendritic cells (DCs), a low level of Zn2+ can induce the activation immunogenic DCs (igDCs), thereby triggering an active T-cell response to propel immune progression rheumatoid arthritis (RA). This finding indicates crucial roles zinc and oxygen homeostasis in during pathogenesis RA. However, very few studies have focused on modulation RA treatment. Proposed herein is DC-targeting immune-regulating strategy igDCs into tolerogenic (tDCs) inhibit subsequent activation, referred as...

Gouty arthritis (GA) is a form of caused by uric acid deposition in the joints that result intense inflammation and pain. Accumulating evidence showed importance sensory neurons signal upon immune cells releasing neuropeptides chemokines to regulate associated immune-inflammatory response. In this study, we investigated significance neuron chemokine signals on inflammation-induced macrophages polarization during GA. Methods: We screened mRNA expression profile GA dorsal root ganglion (DRG)...

Osteoarthritis (OA) is an inflammation-driven degenerative joint disease. Human salivary peptide histatin-1 (Hst1) shows pro-healing and immunomodulatory properties. but its role in OA treatment not fully understood. In this study, we investigated the efficacy of Hst1 inflammation modulation-mediated attenuation bone cartilage damage OA. was intra-articularly injected into a rat knee monosodium iodoacetate (MIA)-induced model. Micro-CT, histological, immunohistochemical analyses showed that...

Cervical andersson lesions (ALs) are rare in patients with ankylosing spondylitis (AS), and even more simultaneous superior cervical atlantoaxial fracture dislocation. Here, we present a case of C1 Jefferson (C1 bilateral posterior arch fracture), C2 odontoid, lateral mass, vertebral (nonclassic hangman traumatic dislocation (AAD) C6/C7 AL long-standing AS spine. The patient AS-related fractures underwent two-stage surgery. stage I surgery involved reduction fixation combined C5/C6/T1/T2...

Photodynamic therapy is a potentially advantageous treatment for non-melanoma skin cancers. We evaluated the clinical response, recurrence and adverse events of photodynamic in situ extramammary Paget's disease 14 male 3 female Chinese patients with 21 lesions. Topical 20% 5-aminolevulinic acid was applied 6 h. Each lesion irradiated 633 nm red light three times, 1 week apart, at total dose 339 J/cm2, followed by assessments 6, 12 24 months. Overall complete response (CR) rates were 52.4%,...