A5023279534- Trypanosoma species research and implications
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Cytomegalovirus and herpesvirus research
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Fish Ecology and Management Studies
- Viral Infections and Outbreaks Research
- interferon and immune responses
- Marine and fisheries research
- Influenza Virus Research Studies
University of Glasgow
2022-2024
Wellcome Centre for Molecular Parasitology
2022-2024
Institute for Biodiversity
2022
Département Santé Animale
2022
Human African trypanosomiasis, or sleeping sickness, is caused by the protozoan parasite Trypanosoma brucei and induces profound reactivity of glial cells neuroinflammation when parasites colonise central nervous system. However, transcriptional functional responses brain to chronic T. infection remain poorly understood. By integrating single cell spatial transcriptomics mouse brain, we identify that triggered are readily detected in proximity circumventricular organs, including lateral 3rd...
Abstract Single molecule fluorescence in situ hybridisation (smFISH) has become a valuable tool to investigate the mRNA expression of single cells. However, it requires considerable amount programming expertise use currently available open-source analytical software packages extract and analyse quantitative data about transcript expression. Here, we present FISHtoFigure, new developed specifically for analysis abundance co-expression QuPath-quantified, multi-labelled smFISH data....
Abstract Human African trypanosomiasis, or sleeping sickness, is caused by the protozoan parasite Trypanosoma brucei and induces profound reactivity of glial cells neuroinflammation when parasites colonise central nervous system. However, transcriptional functional responses brain to chronic T. infection remain poorly understood. By integrating single cell spatial transcriptomics mouse brain, we identified that triggered are readily detected in proximity circumventricular organs, including...
As well as spreading through virions, influenza A viruses (IAVs) can evade antiviral drugs and neutralising antibodies by directly from cell to cell. In culture this occur the induction of intercellular membrane connections known tunnelling nanotube-like structures (TLSs), which are capable trafficking viral genome between cells. Here, we showed that TLSs present at site IAV infections in vivo, then used vitro models ask how IAVs induce their formation. We found TLS cannot be induced...
Abstract Single molecule fluorescence in situ hybridisation (smFISH) has become a valuable tool to investigate the mRNA expression of single cells. However, it requires considerable amount bioinformatic expertise use currently available open-source analytical software packages extract and analyse quantitative data about transcript expression. Here, we present FISHtoFigure, new developed specifically for analysis abundance co-expression QuPath-quantified, multi-labelled smFISH data....
Abstract Human African trypanosomiasis, or sleeping sickness, is caused by the protozoan parasite Trypanosoma brucei and induces profound reactivity of glial cells neuroinflammation when parasites colonise central nervous system. However, transcriptional functional responses brain to chronic T. infection remain poorly understood. By integrating single cell spatial transcriptomics mouse brain, we identified that triggered are readily detected in proximity circumventricular organs, including...