Oral squamous cell carcinoma (OSCC) is one of the most common malignant diseases worldwide, yet its molecular mechanisms are largely unknown. We aimed to construct gene co-expression networks identify key modules and hub genes involved in pathogenesis OSCC.We used dataset GSE30784 by weighted network analysis (WGCNA). Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses were performed Database for Annotation, Visualization Integrated Discovery (DAVID). Hub screened...

Eight new Zn(II) coordination polymers based on flexible 1,4-di(1H-imidazol-1-yl)butane and different dicarboxylates, [Zn(dimb)(suc)·2DMF]n (1), [Zn(dimb)(mbda)·3H2O]n (2), [Zn(dimb)(adip)·DMF·2H2O]n (3), [Zn(dimb)(pma)·2.5H2O]n (4), [Zn2(dimb)(tha)2(H2O)]n (5), [Zn(dimb)(chda)·2H2O]n (6), [Zn(dimb)(obda)·DMF]n (7), [Zn(dimb)(tdga)·CH3OH]n (8) (dimb = 1,4-di(1H-imidazol-1-yl)butane, H2suc succinic acid, H2mbda m-benzenediacetic H2adip adipic H2pma pimelic H2tha thiophene-2,5-dicarboxylic...

Diallyl disulfide (DADs), a natural organic compound, is extracted from garlic and scallion has anti-tumor effects against various tumors. This study investigated the activity of DADs in human osteosarcoma cells mechanisms. MG-63 were exposed to (0, 20, 40, 60, 80, 100 μM) for different lengths time (24, 48, 72 h). The CCK8 assay results showed that inhibited cell viability dose-and time-dependent manner. FITC-Annexin V/propidium iodide staining flow cytometry demonstrated apoptotic ratio...

Abstract Background The incidence of diabetic osteoporosis is increasing. This article evaluates the effect combination treatment with hypoglycemic drug exendin-4 (Ex-4) and vitamin D analog eldecalcitol (ED-71) on improving explores relevant mechanism action. Method Micro-CT, HE staining, immunohistochemistry, qPCR ELISA were used to evaluate impact Ex-4 ED-71 bone formation macrophage polarization in a mouse model vivo. Immunofluorescence, flow cytometry characterize type macrophages...

The solvothermal reaction of CuI with 1,4-bis(pyridin-4-ylmethyl)piperazine (bpmp) in different stoichiometric ratios gave rise to the isolation two copper(I) iodide coordination polymers, namely [Cu4I4(bpmp)2]n (1) and [Cu4I4(bpmp)3]n (2). Complex 1 exhibits a 2D non-interpenetrated 44-sql net constructed from cubic [Cu4I4] cluster building units, whereas 2 is → 3D inclined interpenetrated incorporating stairstep clusters. Both could also be obtained under liquid-assisted grinding...

Dentin, a complex, living, and porous mineral substance, is produced by the mineralization of predentin, which secreted odontoblasts. This substance crucial for maintaining health teeth. However, specific function vitamin D receptor (Vdr) in odontoblasts, dentin homeostasis, its interaction with Wnt signaling pathway during apposition not well understood. In this study, we employed Vdr transgenic knockout mice to study dental effects observed enlarged pulp cavities, diminished dentin,...

Oral squamous cell carcinoma (OSCC) is a malignant disease. Methylation plays key role in the etiology and pathogenesis of OSCC. The goal this study was to identify aberrantly methylated differentially expressed genes (DEGs) OSCCs, explore underlying mechanisms tumorigenesis by using integrated bioinformatic analysis. Gene expression profiles (GSE30784 GSE38532) were analyzed R software obtain DEGs. Functional enrichment analysis screened performed DAVID software. Protein⁻protein interaction...

HuR (human antigen R)-an RNA-binding protein-is involved in regulating mRNA stability by binding adenylate-uridylate-rich elements. This study explores the role of regulation smooth muscle contraction and blood pressure. Approach Results: Vascular HuRSMKO (smooth muscle-specific knockout) mice were generated crossbreeding HuRflox/flox with α-SMA (α-smooth actin)-Cre mice. As compared CTR (control) mice, showed hypertension cardiac hypertrophy. levels decreased aortas from hypertensive...

Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors worldwide, and its prognosis still not optimistic. Oxaliplatin a type platinum chemotherapeutic agent, but treatment effects on OSCC molecular mechanisms have been fully elucidated. Parthanatos, unique form death, plays an important role in variety physiological pathological processes. This study aims to investigate whether oxaliplatin inhibits by inducing parthanatos. Our results showed that inhibited...

Adhesion of epithelial cells to basement membranes (BM) occurs through two major structures: actin-associated focal contacts and keratin-associated hemidesmosomes, both which form on laminin-332 (Ln-332). In epithelial-derived cancer cells, additional actin-linked structures with putative adhesive properties, invadopodia, are frequently present mediate BM degradation. A recent study proposed that invasion requires a proper combination invadopodia for invading gain traction degraded BM,...

During the course of screening natural sesquiterpenoids for new antitumor agents, two novel compounds, fischerisin A (1) and B (2), were isolated from roots Ligularia fischeri. Their structures elucidated by interpretation their IR, high resolution-mass spectrometry (HR-MS), 1D- 2D-NMR data. Fischerisin are first representatives a sesquiterpenoid-geranylhydroquinone hybrid, both compounds exhibited in vitro cytotoxicity against cultured human oral epidermoid carcinoma (KB) breast cancer...

Lipopolysaccharide (LPS)-induced osteoblast apoptosis is a prominent factor to the defect in periodontal tissue repair disease. LPS challenge contributes production of reactive oxygen species (ROS) periodontitis, and peroxiredoxin 1 (Prx1) an antioxidant protein that protect cells against oxidative damage from ROS. Without stimulation, apoptotic rates were higher both Prx1 knockout (Prx1KO) overexpression (Prx1OE) compared with wild type. After intracellular ROS Prx1KO showed highest level...

Oral cancer is one of the most common malignancies head and neck, approximately 90% oral cancers are squamous cell carcinomas (OSCCs). The purinergic P2Y2 receptor upregulated in breast cancer, pancreatic colorectal liver but its role OSCC still unclear. Here, we examined effects on invasion migration cells (SCC15 CAL27). BALB/c mouse model was used to observe involvement with tumors vivo. P2Y2, Src, EGFR highly expressed tissues lines. Stimulation ATP significantly enhanced cells, activity...

The annual discharge (6–7 million tons per year) of Chinese medicine industry waste (CMIW) is large and harmful. CMIW with a high moisture content can be effectively treated by hydrothermal carbonization (HTC) technology. Compared CMIW, the volume number pores prepared hydrochar increased significantly after alkali etching (AE), they had abundant oxygen-containing functional groups. These properties provide physical chemical adsorption sites, improving adsorbent activity alkaline (AE-CMIW...

Peroxiredoxin 1 (Prdx1), a vital antioxidant enzyme, has been proven to play an important role in the occurrence and development of cancers, but its effects on oral squamous cell carcinoma (OSCC) remain unclear. Here, we performed bioinformatics analysis immunohistochemical (IHC) staining confirm that Prdx1 was higher OSCC tissues than normal tissues. Consistently, RT-PCR Western blot showed elevated expression lines compared human keratinocytes (HOK), which could be knockdown by small...

Abstract . The solvothermal reaction between cuprous iodide and the rigid triangular imidazole ligand in mixed N , ′‐dimethylacetamide (DMA)‐acetonitrile solvent leads to isolation of 3D metal‐organic framework [(Cu 4 I ) 3 (TIPA) ] · 7DMA ( 1 [TIPA = tri(4‐imidazolylphenyl) amine], which was characterized by elemental analysis, IR spectroscopy, powder X‐ray diffraction, single‐crystal diffraction. Topologically, structure is an unprecedented 3,3,4,4‐connected net with a point symbol...