A5081298976- Hippo pathway signaling and YAP/TAZ
- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Angiogenesis and VEGF in Cancer
- Intracranial Aneurysms: Treatment and Complications
- Vascular Malformations Diagnosis and Treatment
- Axon Guidance and Neuronal Signaling
- Barrier Structure and Function Studies
- Intracerebral and Subarachnoid Hemorrhage Research
- Neurogenesis and neuroplasticity mechanisms
- Circular RNAs in diseases
- Pluripotent Stem Cells Research
- Growth Hormone and Insulin-like Growth Factors
- CRISPR and Genetic Engineering
- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Phosphodiesterase function and regulation
- Neurological Disease Mechanisms and Treatments
- Cellular Mechanics and Interactions
Goethe University Frankfurt
2015-2024
Institute of Cell Biology
2016
Focus (Germany)
2015
University Medical Center of the Johannes Gutenberg University Mainz
2015
Johannes Gutenberg University Mainz
2015
Max Planck Institute for Brain Research
2015
Institute of Molecular Biology
2015
Royal Netherlands Academy of Arts and Sciences
2015
University Medical Center Utrecht
2015
Hubrecht Institute for Developmental Biology and Stem Cell Research
2011-2014
Background— Myocardial infarction leads to cardiac remodeling and development of heart failure. Insufficient myocardial capillary density after has been identified as a critical event in this process, although the underlying mechanisms angiogenesis are mechanistically not well understood. Methods Results— Here, we show that small noncoding RNA microRNA-24 (miR-24) is enriched endothelial cells considerably upregulated ischemia. MiR-24 induces cell apoptosis, abolishes network formation on...
During cardiovascular development, tight spatiotemporal regulation of molecular cues is essential for controlling endothelial cell (EC) migration. Secreted class III Semaphorins play an important role in guidance neuronal migration and were lately linked to regulating development. Recently, SEMA3D gene disruptions associated with defects patients; however, the mechanisms action not revealed. Here we show first time that Sema3d regulates collective EC zebrafish through two separate...
Blood vessel morphogenesis is driven by coordinated endothelial cell behaviors. Active remodeling of cell-cell junctions promotes cellular plasticity while preserving vascular integrity. Here, we analyze the dynamics adherens during lumen formation in angiogenic sprouts vivo. Live imaging zebrafish reveals that expansion accompanied transient finger-shaped junctions. Junctional fingers are positively regulated blood pressure, whereas flow inhibition prevents their formation. Using...
In this study, we explored the existence of a transcriptional network co-regulated by E2F7 and HIF1α, as show that expression E2F7, like is induced in hypoxia, because previously reported ability to interact with HIF1α. Our genome-wide analysis uncovers directly controlled HIF1α demonstrates both stimulatory repressive functions -E2F7 complex. Among reveal Neuropilin 1 (NRP1) HIF1α-E2F7 repressed gene. By performing vitro vivo reporter assays demonstrate mediated NRP1 repression depends on...
Loss-of-function variants in CCM1/KRIT1, CCM2, and CCM3/PDCD10 are associated with autosomal dominant cerebral cavernous malformations (CCMs). CRISPR/Cas9-mediated CCM3 inactivation human endothelial cells (ECs) has been shown to induce profound defects cell-cell interaction as well actin cytoskeleton organization. We here show that impairs fibronectin expression consequently leads reduced fibers the extracellular matrix. Despite complexity high molecular weight of fibrils, our vitro model...
Cerebral cavernous malformations (CCMs) are prevalent slow-flow vascular lesions which harbour the risk to develop intracranial haemorrhages, focal neurological deficits, and epileptic seizures. Autosomal dominantly inherited CCMs were found be associated with heterozygous inactivating mutations in 3 genes, CCM1 (KRIT1), CCM2 (MGC4607), CCM3 (PDCD10) 1999, 2003 2005, respectively. Despite availability of high-throughput sequencing techniques, no further CCM gene has been published since....
Abstract Veins have emerged as the origin of all other endothelial cell subtypes needed to expand vascular networks during developmental and pathological neoangiogenesis. Here, we uncover significant role angioneurin Fibronectin Leucine Rich Transmembrane protein (FLRT) 2 in central nervous system (CNS) development mouse. Early postnatal FLRT2 deletion reveals specific defects retinal veins, impacting proliferation, sprouting polarity that result reduced tip cells at front. interacts with...
Abstract Veins have emerged as the origin of all other endothelial cell subtypes needed to expand vascular networks during developmental and pathological neoangiogenesis. Here, we uncover role angioneurin Fibronectin Leucine Rich Transmembrane protein (FLRT) 2 in central nervous system (CNS) development mouse. Early postnatal FLRT2 deletion reveals specific defects retinal veins, impacting proliferation, sprouting polarity that result reduced tip cells at front. interacts with VE-cadherin...
Abstract Blood vessel morphogenesis is driven by coordinated endothelial cell behaviors, which depend on dynamic cell-cell interactions. Remodeling of junctions promote morphogenetic cellular events while preserving vascular integrity. Here, we have analyzed the dynamics during lumen formation in angiogenic sprouts. By live-imaging intersegmental blood vessels zebrafish, demonstrate that expansion accompanied transient finger-shaped junctions. Formation and maintenance these junctional...
Blood vessel morphogenesis is driven by coordinated endothelial cell behaviors, which depend on dynamic cell-cell interactions. Remodeling of junctions promote morphogenetic cellular events while preserving vascular integrity. Here, we have analyzed the dynamics during lumen formation in angiogenic sprouts. By live-imaging intersegmental blood vessels zebrafish, demonstrate that expansion accompanied transient finger-shaped junctions. Formation and maintenance these junctional fingers are...
cAMP generated in response to β-adrenoceptor stimulation modulates excitation-contraction coupling via activation of PKA. It is now widely accepted that signalling compartmentalised the heart and PDEs, only degrading enzyme, play a central role spatial control signal propagation. We have previously demonstrated PKA-I PKA-II isoforms are selectively activated by spatially confined pools leading phosphorylation specific downstream targets. has been suggested selective deregulation cAMP/PKA...