A5082802728- Estrogen and related hormone effects
- Menopause: Health Impacts and Treatments
- Nitric Oxide and Endothelin Effects
- Atherosclerosis and Cardiovascular Diseases
- Inflammatory mediators and NSAID effects
- MicroRNA in disease regulation
- Bone Metabolism and Diseases
- Antioxidant Activity and Oxidative Stress
- Ovarian function and disorders
- Neuroscience and Neuropharmacology Research
- Angiogenesis and VEGF in Cancer
- Reproductive System and Pregnancy
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Reproductive Biology and Fertility
- Hormonal Regulation and Hypertension
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Sulfur Compounds in Biology
- Circular RNAs in diseases
- Phytoestrogen effects and research
- Metabolism and Genetic Disorders
- Redox biology and oxidative stress
- Ion channel regulation and function
- Renin-Angiotensin System Studies
- Neuroendocrine regulation and behavior
Universitat de València
2013-2024
INCLIVA Health Research Institute
2013-2024
Universidade Nova de Lisboa
2024
Universidade Lusófona do Porto
2023
Biomedical Research Institute
2015-2018
Instituto de Química y Fisicoquímica Biológicas
2016
Consejo Nacional de Investigaciones Científicas y Técnicas
2016
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2012-2016
Instituto Nacional del Tórax
2016
Hospital Clínico Universitario de Valencia
2004-2015
Ammonia is considered the main agent responsible for neurological alterations in hepatic encephalopathy. It was suggested that ammonia toxicity mediated by activation of N -methyl-d-aspartate (NMDA) receptors. The aim this work to assess, vivo brain microdialysis freely moving rats, whether acute intoxication leads NMDA receptors cerebellum rat . We measured effects on neuronal glutamate-nitric oxide-cyclic guanosine monophosphate (cGMP) pathway, measuring ammonia-induced increase...
Injection of large doses ammonium salts lead to the rapid death animals. However, molecular mechanisms involved in ammonia toxicity remain be clarified. We have tested effect injecting 7 mmol/kg acetate on production superoxide and activities some antioxidant enzymes rat liver, brain, erythrocytes plasma. Glutathione peroxidase, dismutase catalase were decreased liver brain (both cytoso-lic mitochondrial fractions) also blood red cells, while glutathione reductase activity remained...
Previous experiments in our laboratory suggested that ammonium toxicity could be mediated by the NMDA type of glutamate receptors. To assess this hypothesis we tested if MK‐801, a specific antagonist receptor, is able to prevent toxicity. Mice and rats were injected i.p. with 12 7 mmol/kg acetate, respectively, 73% mice 70% died. However, when animals 2 mg/kg 15 min before injection, only 5% 15% The remarkable protection afforded MK‐801 indicates ammonia receptor.
The incidence of cardiovascular diseases in premenopausal women is lower than men or postmenopausal women. This study reports the discovery a low grade systemic inflammation, including monocyte adhesion to arterial endothelium, elicited by menopause estrogen depletion. Chronic treatment with dose 17-beta-estradiol inhibition renin-angiotensin system reduced this inflammation. Using an vitro flow chamber human and venous endothelial cells, we found that leukocytes from healthy were more...
REVIEW article Front. Physiol., 06 June 2012Sec. Vascular Physiology Volume 3 - 2012 | https://doi.org/10.3389/fphys.2012.00165
Abstract Despite the promising value of miRNAs in diagnostic and prognostic cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances studies may interfere with miRNA profile affect results. This study determines if blood starting material is a source variance by performing paired comparison plasma serum expression primary associated CVD. Circulating yield was similar both serum, although significant increase observed patients...
Abstract The aim of this work was to assess whether ammonia concentrations similar the increase found in brain hyperammonemic rats (100 μ m ), impair N ‐methyl‐ d ‐aspartate (NMDA) receptor‐mediated signal transduction. We first measured glutamate neurotoxicity, which these neurons is mediated by activation NMDA receptors, as an initial parameter reflecting pathways. Long‐term treatment cultured with prevents glutamate‐induced neuronal death. EC 50 20 , and at 100 protection complete....
Abstract Angiotensin II (Ang-II) displays inflammatory activity and is implicated in several cardiovascular disorders. This study evaluates the effect of cis- trans (t)-resveratrol (RESV) two vivo models vascular inflammation identifies cardioprotective mechanisms that underlie them. In vivo, Ang-II–induced arteriolar leukocyte adhesion was inhibited by 71% t-RESV (2.1 mg/kg, i.v.), but not affected cis-RESV. Because estrogens influence rennin-angiotensin system, chronic treatment with (15...
Objective— Our study aims to determine the role of time menopause on vascular inflammation biomarkers and how it affects their modulation by estrogen raloxifene in postmenopausal women. Methods Results— Uterine arteries from 68 women were divided into 3 segments cultured for 24 hours tissue culture media containing 17β-estradiol (100 nmol/L), or vehicle. Assessment arterial concentration 13 inflammatory was performed multiplex immunobead-based assay. Aging per se has a positive correlation...
Estradiol (E 2 ) acts on the endothelium to promote vasodilatation through release of several compounds, including prostanoids, which are products arachidonic acid metabolism. Among these, prostacyclin (PGI2) and thromboxane A2 (TXA2) exert opposite effects vascular tone. The role different estrogen receptors (ERs) in PGI2/TXA2 balance, however, has not been fully elucidated. Our study sought uncover whether E enhances basal production PGI2 or TXA2 cultured human umbilical vein endothelial...
Aims of this study were to analyze the long-term effects postovulatory aging mouse oocytes on 1) reproductive traits parental (F0) and first (F1)-generation females (pregnancy rate, gestation length, litter size, perinatal death, sex ratio offspring) 2) developmental behavioral variables F1 second-generation (F2) offspring (birth weight gain during preweaning development, postnatal day attainment immediate righting, spontaneous motor activity, passive active conditioned learning ability)....
Aims Aging is among the major causes for lack of cardiovascular protection by estrogen (E2) during postmenopause. Our study aims to determine mechanisms whereby aging changes E2 effects on nitric oxide (NO) production in a mouse model accelerated senescence (SAM). Methods and Results Although we found no differences NO females SAM prone (SAMP, aged) compared resistant (SAMR, young), either DAF-2 fluorescence or plasmatic nitrite/nitrate (NO2/NO3), both cases, treatment increased SAMR but had...
Introduction: Circulating extracellular histones acquire relevance as cytotoxic mediators in sepsis. Extracellular act damage-associated molecular patterns (DAMPs), which induce oxidative stress and NLRP3 inflammasome activation. Inflammasome mediates pyroptosis, a programmed cell death mechanism that produces inflammation. Despite evidence for activation immune cells during sepsis, it was unknown whether can produce endothelial inflammasomes Methods: We used human umbilical vein (HUVEC) to...
Cardiovascular manifestations are frequent findings in patients with thyroid hormone disorders. Nitric oxide (NO) plays a key role vascular, endothelial-mediated relaxation. NO is synthesized from l-arginine by synthase, an enzyme inhibited endogenous compounds, mainly asymmetric dimethylarginine [asymmetric NG,NG-dimethyl-l-arginine (ADMA)]. The aim of our work was to investigate whether plasma and concentrations production altered hypo- hyperthyroid patients, compared control subjects....
Asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide (NO) synthase, is a novel cardiovascular risk factor produced by endothelial cells. ADMA levels are mainly regulated the activity dimethylaminohydrolases (DDAH). Endothelial release increased in presence oxidized LDL cholesterol (oxLDL), whereas estrogens stimulate NO production cells increasing both expression and synthase reducing levels. Thus, aim present study was to evaluate estradiol effects on DDAH/ADMA/NO...
Vascular effects of estradiol are being investigated because there controversies among clinical and experimental studies. DNA microarrays were used to investigate global gene expression patterns in cultured human umbilical vein endothelial cells (HUVEC) exposed 1 nmol/L for 24 hours. When compared control, 187 genes identified as differentially expressed with 1.9-fold change threshold. Supervised principal component analysis hierarchical cluster revealed the differences between control...