A5063110148- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Angiogenesis and VEGF in Cancer
- Estrogen and related hormone effects
- Peripheral Artery Disease Management
- Atherosclerosis and Cardiovascular Diseases
- Extracellular vesicles in disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nitric Oxide and Endothelin Effects
- Hormonal Regulation and Hypertension
- Coronary Interventions and Diagnostics
- Kruppel-like factors research
- Cell Adhesion Molecules Research
- E-Learning and Knowledge Management
- Immune cells in cancer
- Educational Innovations and Technology
- Diabetic Foot Ulcer Assessment and Management
- interferon and immune responses
- Adipokines, Inflammation, and Metabolic Diseases
- Inflammasome and immune disorders
- IL-33, ST2, and ILC Pathways
- Adipose Tissue and Metabolism
- Social Issues and Sustainability
- Bioactive natural compounds
Brigham and Women's Hospital
2020-2024
Harvard University
2020-2024
INCLIVA Health Research Institute
2015-2024
Universitat de València
2015-2024
Biomedical Research Institute
2016-2018
Parc Científic de la Universitat de València
2018
Instituto de Salud Carlos III
2018
Instituto de Química y Fisicoquímica Biológicas
2016
Consejo Nacional de Investigaciones Científicas y Técnicas
2016
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2016
Abstract The retinal pigment epithelium ( RPE ), a monolayer located between the photoreceptors and choroid, is constantly damaged by oxidative stress, particularly because of reactive oxygen species ROS ). As , its physiological functions, essential for survival retina, any sustained damage may consequently lead to loss vision. Exosomes are small membranous vesicles released into extracellular medium numerous cell types, including cells. Their cargo includes genetic material proteins,...
Long non-coding RNAs (lncRNAs) are emerging regulators of pathophysiological processes including atherosclerosis. Using RNA-seq profiling the intima lesions, here we identify a macrophage-specific lncRNA MAARS (Macrophage-Associated Atherosclerosis Sequence). Aortic expression increases by 270-fold with atherosclerotic progression and decreases regression 60%. knockdown reduces lesion formation 52% in LDLR
Abstract Despite the promising value of miRNAs in diagnostic and prognostic cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances studies may interfere with miRNA profile affect results. This study determines if blood starting material is a source variance by performing paired comparison plasma serum expression primary associated CVD. Circulating yield was similar both serum, although significant increase observed patients...
Rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for disease 2019 (COVID-19), has led to a global pandemic, failures local health care systems, and economic recession. MicroRNAs (miRNAs) have recently emerged as important regulators viral pathogenesis, particularly among RNA viruses, but impact host miRNAs on SARS-CoV-2 infectivity remains unknown. In this study, we utilize combination powerful bioinformatic prediction algorithms miRNA...
Objective: Vascular smooth muscle cell (VSMC) plasticity plays a critical role in the development of atherosclerosis. Long noncoding RNAs (lncRNAs) are emerging as important regulators vessel wall and impact cellular function through diverse interactors. However, lncRNAs regulating VSMCs atherosclerosis remains unclear. Approach Results: We identified VSMC-enriched lncRNA cardiac mesoderm enhancer-associated RNA (CARMN) that is dynamically regulated with progression In both mouse human...
Diabetes-associated atherosclerosis involves excessive immune cell recruitment and plaque formation. However, the mechanisms remain poorly understood. Transcriptomic analysis of aortic intima in Ldlr−/− mice on a high-fat, high-sucrose-containing (HFSC) diet identifies macrophage-enriched nuclear long noncoding RNA (lncRNA), MERRICAL (macrophage-enriched lncRNA regulates inflammation, chemotaxis, atherosclerosis). expression increases by 249% intimal lesions during progression. lncRNA-mRNA...
Introduction: Circulating extracellular histones acquire relevance as cytotoxic mediators in sepsis. Extracellular act damage-associated molecular patterns (DAMPs), which induce oxidative stress and NLRP3 inflammasome activation. Inflammasome mediates pyroptosis, a programmed cell death mechanism that produces inflammation. Despite evidence for activation immune cells during sepsis, it was unknown whether can produce endothelial inflammasomes Methods: We used human umbilical vein (HUVEC) to...
Estrogen signalling plays an important role in vascular biology as it modulates vasoactive and metabolic pathways endothelial cells. Growing evidence has also established microRNA (miRNA) key regulators of function. Nonetheless, the estrogen regulation on miRNA profile cells is poorly understood. In this study, we aimed to determine how human explore different receptors (ERα, ERβ GPER) expression by estrogen.We used microarrays global umbilical vein (HUVEC) exposed a physiological...
Perivascular fibrosis, characterized by increased amount of connective tissue around vessels, is a hallmark for vascular disease. Ang II (angiotensin II) contributes to disease and end-organ damage via promoting T-cell activation. Despite recent data suggesting the role T cells in progression perivascular underlying mechanisms are poorly understood.
Patients with peripheral artery disease (PAD) and diabetes constitute a high risk population for development of critical limb ischemia (CLI) amputation, although the underlying mechanisms remain poorly understood.Comparison dysregulated microRNAs from diabetic human subjects PAD mice revealed conserved microRNA, miR-130b-3p. In vitro angiogenic assays demonstrated miR-130b rapidly promoted proliferation, migration, sprouting in endothelial cells (ECs), whereas inhibition exerted...
Abstract Extracellular histones are mediators of inflammation, tissue injury and organ dysfunction. Interactions between circulating vascular endothelial cells key events in histone‐mediated pathologies. Our aim was to investigate the implication extracellular production major vasoactive compounds released by human ( HUVEC s), prostanoids nitric oxide NO ). exposed increasing concentrations (0.001 100 μg/ml) for 4 hrs induced prostacyclin PGI 2) a dose‐dependent manner decreased thromboxane...
Patients with peripheral artery disease (PAD) and diabetes have the highest risk of critical limb ischemia (CLI) amputation, yet underlying mechanisms remain incompletely understood. MicroRNA (miRNA) sequencing plasma from diabetic patients or without CLI was compared to mice acute subacute identify conserved miRNAs. miRNA-KO on high-fat diet were generated explore impact CLI. Comparison dysregulated miRNAs individuals PAD revealed miR-181 family members. High-fat–fed, Mir181a2b2-KO had...
Abstract Aims Diabetes leads to dysregulated macrophage immunometabolism, contributing accelerated atherosclerosis progression. Identifying critical factors restore metabolic alterations and promote resolution of inflammation remains an unmet goal. MicroRNAs orchestrate multiple signalling events in macrophages, yet their therapeutic potential diabetes-associated unclear. Methods results miRNA profiling revealed significantly lower miR-369-3p expression aortic intimal lesions from Ldlr–/–...
Abstract The culture of endothelial progenitor cells ( EPC ) provides an excellent tool to research on biology and vascular regeneration vasculogenesis. use different protocols obtain cultures makes it difficult comparable results in groups. This work offers a systematic comparison the main variables most commonly used for isolation, functional evaluation. Peripheral blood samples from healthy individuals were recovered mononuclear cultured. Different recovery conditions tested: volume,...