A5020832252- Protein Tyrosine Phosphatases
- Atherosclerosis and Cardiovascular Diseases
- Redox biology and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Heavy Metal Exposure and Toxicity
- Macrophage Migration Inhibitory Factor
- Galectins and Cancer Biology
- Cancer-related molecular mechanisms research
- Sulfur Compounds in Biology
- Phagocytosis and Immune Regulation
- Cardiovascular Disease and Adiposity
- Osteoarthritis Treatment and Mechanisms
- Alzheimer's disease research and treatments
- Angiogenesis and VEGF in Cancer
- Aluminum toxicity and tolerance in plants and animals
- Nanoparticles: synthesis and applications
- Autophagy in Disease and Therapy
- Congenital heart defects research
- Mesenchymal stem cell research
- Smoking Behavior and Cessation
- Forensic Toxicology and Drug Analysis
- Neuroinflammation and Neurodegeneration Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Asthma and respiratory diseases
Boston University
2020-2024
Boston Medical Center
2023
SUNY Polytechnic Institute
2019-2020
Zero to Three
2018
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2018
King George's Medical University
2012-2017
Protein-tyrosine phosphatases (PTPs) counteract protein tyrosine phosphorylation and cooperate with receptor-tyrosine kinases in the regulation of cell signaling. PTPs need to undergo oxidative inhibition for activation cellular cascades protein-tyrosine kinase following growth factor stimulation. It has remained enigmatic how such oxidation can occur presence potent reducing systems. Here, using vitro biochemical assays purified, recombinant protein, along experiments adenocarcinoma line...
Aluminium (Al) is the third most abundant element in earth's crust and its compounds are used form of house hold utensils, medicines antiperspirant etc. Increasing number evidences suggest involvement Al+3 ions a variety neurodegenerative disorders including Alzheimer's disease. Here, we have attempted to investigate role Al endoplasmic reticulum stress regulation p53 during neuronal apoptosis using neuroblastoma cell line. We observed that caused oxidative by increasing ROS production...
Pod-based electronic (e-) cigarettes more efficiently deliver nicotine using a protonated formulation. The cardiovascular effects associated with these devices are poorly understood. We evaluated whether pod-based e-liquids and their individual components impair endothelial cell function. isolated cells from people who pod users (n = 10), tobacco never 7), combustible cigarette 6). After structured use, had lower acetylcholine-mediated nitric oxide synthase (eNOS) activation compared was...
Silica nanoparticles (SiNPs) are being used increasingly in biomedical and industrial fields; however, their adverse effects on human health have not been fully investigated. In this study, we focused some of the toxicological aspects SiNPs by studying oxidative stress pro-inflammatory responses frontal cortex, corpus striatum hippocampus regions rat brain. Wistar rats were exposed to size 80 nm 10 at a dose 150 µg/50 µL phosphate-buffered saline/rat for 30 days. The results indicated...
Abstract Aims Diabetes leads to dysregulated macrophage immunometabolism, contributing accelerated atherosclerosis progression. Identifying critical factors restore metabolic alterations and promote resolution of inflammation remains an unmet goal. MicroRNAs orchestrate multiple signalling events in macrophages, yet their therapeutic potential diabetes-associated unclear. Methods results miRNA profiling revealed significantly lower miR-369-3p expression aortic intimal lesions from Ldlr–/–...
hronic obstructive pulmonary disease (COPD) refers to a group of lung diseases characterized by airway inflammation and tissue destruction with non-reversible airflow limitation.Airflow limitation is generally progressive associated an abnormal response the lungs noxious particles or gases such as cigarette smoke, coal mining dust, diesel exhaust particles, fumes from burning biomass fuels for cooking heating that leads chronic inflammation. 1There broad range inflammatory cells like...
We have evaluated the cardiomyogenic potential of clonal populations human bone marrow mesenchymal stem cells (BM-MSC). Four rapidly proliferating clones BM-MSC were obtained from BM a healthy donor which then treated with 5-azacytidine and for expression GATA-4, NKx-2.5, FOG-2, TDGF-1, β-MHC, MEF2D NPPA genes cTnT, Desmin β-MHC proteins. Of four (i) Clone-1 had high GATA-4 (1.89 fold (p<0.05), Nkx2.5 (2.29 fold; p<0.05), FOG2 (2.76 TDGF1 (6.97 fold, p<0.005), βMHC (10.22 MEF-2D (1.91...
Endothelial cell (EC) dysfunction is prevalent in individuals with insulin resistance and diabetes, characterized by diminished phosphorylation of endothelial nitric oxide synthase (p-eNOS) reduced (NO) production the endothelium. Despite its common occurrence, fundamental mechanism underlying EC remains incompletely understood. Dysregulation microRNAs (miRNAs) has been implicated biology metabolic disorders, yet studies exploring functional relevance these miRNAs are limited. In this...
Background: Traditional combustible cigarette (combustible) use induces endothelial dysfunction accelerating cardiovascular disease progression. Young adults frequently electronic cigarettes (e-cig) that is linked to impaired cell (EC) function. We aimed EC gene expression analysis explore altered pathways across patterns of tobacco product use. Methods: Healthy aged 18-45 who regularly e-cig (sole or dual), combustible, and non-use controls were with forearm vein biopsy magnetic bead...
Abstract The reversible oxidation of protein tyrosine phosphatases (PTPs) impairs their ability to dephosphorylate substrates in vivo. This transient inactivation PTPs occurs as conserved catalytic cysteine residue reacts with cellular oxidants thereby abolishing the this reactive attack phosphate target substrate. Hence, vivo, inhibition specific response regulated and localized rises enables phospho‐dependent signaling. We present assays that measure endogenous activity become transiently...
Patients with T2DM display endothelial dysfunction that predicts cardiovascular risk. We have previously demonstrated reduced eNOS activation in cells (ECs) isolated from patients T2DM. sought to identify drivers of EC using a combination proteomic and transcriptomic approaches by measuring: serum O-link panels II III along insulin-mediated phosphorylation miRNA profiling (NextGen sequencing) ECs. In 69 (37 32 age- sex-similar non-T2DM controls) analysis identified 35 upregulated 6...
The Protein Tyrosine Phosphatase (PTP) family of phosphatases regulates cell signaling through dephosphorylation serine, threonine and tyrosine residues downstream effector proteins. role PTPs is very well known in normal functions, as disease states such diabetes cardiovascular diseases. We previously reported on the phosphotyrosine recognition loop (PTBL) PTP1B protein-protein interactions (Londhe et al.2020, Nat. Chem. Biol., 16(2):122-125) showed that interfering with between...