A5040721043- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Cytomegalovirus and herpesvirus research
- Retinal Imaging and Analysis
- Adipose Tissue and Metabolism
- Advanced Glycation End Products research
- melanin and skin pigmentation
- Extracellular vesicles in disease
- Autophagy in Disease and Therapy
- Connexins and lens biology
- Retinal and Optic Conditions
- Eicosanoids and Hypertension Pharmacology
- Peroxisome Proliferator-Activated Receptors
- Retinoids in leukemia and cellular processes
- Adenosine and Purinergic Signaling
- Glaucoma and retinal disorders
- Trace Elements in Health
- Sirtuins and Resveratrol in Medicine
- Saffron Plant Research Studies
- Alcohol Consumption and Health Effects
- Biochemical effects in animals
- Calcium signaling and nucleotide metabolism
- RNA modifications and cancer
- Heme Oxygenase-1 and Carbon Monoxide
University of Alicante
2018-2025
Universidad de Salamanca
2023
Universidad Europea
2019
Valencia Catholic University Saint Vincent Martyr
2013-2016
Universitat de València
2014-2016
Abstract The retinal pigment epithelium ( RPE ), a monolayer located between the photoreceptors and choroid, is constantly damaged by oxidative stress, particularly because of reactive oxygen species ROS ). As , its physiological functions, essential for survival retina, any sustained damage may consequently lead to loss vision. Exosomes are small membranous vesicles released into extracellular medium numerous cell types, including cells. Their cargo includes genetic material proteins,...
This study assessed retinal cells in the macula of human donors with diabetes or without retinopathy. Seventeen donor retinas were classified as mellitus (DM, n = 7), diabetic retinopathy (DR, 3), control (n 8). Macular transversal sections analyzed for photoreceptors, bipolar cells, horizontal ganglion their synaptic connections, and Müller using immunohistochemistry confocal microscopy. The densities thickness inner plexiform layer (IPL) quantified around fovea. In macula, cone...
Significance The retina is considered a window to the brain, and retinal degenerative diseases involve same mechanisms as those of other neurodegenerative disorders. Neuronal degeneration complex process involving environmental stress, which can affect vulnerable neurons. High-fat diet–induced metabolic alterations may influence homeostasis exacerbate diseases. This study provides evidence that short-term high-fat feeding promotes glucose intolerance, gut microbiome dysbiosis, oxidative...
Retinal pigment epithelium has a crucial role in the physiology and pathophysiology of retina due to its location metabolism. Oxidative damage been demonstrated as pathogenic mechanism several retinal diseases, reactive oxygen species are certainly important by-products ethanol (EtOH) Autophagy shown exert protective effect different cellular animal models. Thus, our model, EtOH treatment increases autophagy flux, concentration-dependent manner. Mitochondrial morphology seems be clearly...
ARPE-19 retinal pigment epithelial cells cultured in a medium containing 35 mM D-glucose led to an augmented ROS formation and release of vascular endothelial factor (VEGF)-containing exosomes compared 5 (standard medium). Exposing these the melanocortin receptor agonist (MCR5) PG-901 (10−10M), for 9 d reduced generation, number released their VEGF content. In contrast, incubating with MCR1 BMS-470539 (10−5 M) or mixed MCR3/4 MTII (0.30 nmol) did not produce any significant decrease levels....
Purpose: Retinitis pigmentosa (RP) is a blinding neurodegenerative disease of the retina that can be affected by many factors. The present study aimed to analyze effect different environmental light intensities in rd10 mice retina. Methods: C57BL/6J and were bred housed under three intensities: scotopic (5 lux), mesopic (50 photopic (300 lux). Visual function was studied using electroretinography optomotor testing. structural morphological integrity retinas evaluated optical coherence...
Purpose: Central Areolar Choroidal Dystrophy (CACD) is a monogenic hereditary retinal disorder characterized by progressive degeneration. In most cases, CACD presents an autosomal dominant inheritance pattern in which patients carry single mutation the peripherin (PRPRH2) gene. This study functional, immunohistochemical and transcriptomic analysis of retinas mouse model carrying p.Arg195Leu PRPH2 Methods: Neural from Prph2 WT/WT WT/KI mice were used at different ages. Electroretinogram (ERG)...
Background/Aims: It is well established that oxidative stress and inflammation are common pathogenic features of retinal degenerative diseases.ITH12674 a novel compound induces the transcription factor Nrf2; in so doing, molecule exhibits anti-inflammatory, antioxidant properties, affords neuroprotection rat cortical neurons subjected to stress.We here tested hypothesis ITH12674 could slow degeneration causes blindness rd10 mice, model retinitis pigmentosa.Methods: Animals were...
Different ocular alterations have been described in patients with coronavirus disease 2019 (COVID-19). Our aim was to determine whether COVID-19 affected retinal cells and establish correlations clinical parameters.Retinal sections flat-mount retinas from human donors (n = 16) controls 15) were immunostained. The location of angiotensin-converting enzyme 2 (ACE2) the morphology microglial cells, Müller astrocytes, photoreceptors analyzed by confocal microscopy. Microglial quantification area...
Ischemia is the main cause of cell death in retinal diseases such as vascular occlusions, diabetic retinopathy, glaucoma, or retinopathy prematurity. Although excitotoxicity considered primary mechanism during an ischemic event, antagonists glutamatergic receptors have been unsuccessful clinical trials with patients suffering ischemia stroke. Our purpose was to analyze if transient receptor potential channel 7 (TRPM7) could contribute dysfunction pathologies associated ischemia. By using...
Purpose: Cytochrome p450 2E1 (CYP2E1) is a detoxifying enzyme with particular affinity for ethanol (EtOH) expressed in several tissues. Although CYP2E1 has been identified human RPE, nothing known about its metabolic activity. Expression of and activity after EtOH exposure have studied RPE ARPE-19 cells. Methods: Ethanol-induced mRNA expression was analyzed by RT-PCR quantitative PCR (qPCR) from donor as well protein determined Western blot. Cytoplasmic location also demonstrated...
The retinal pigment epithelium (RPE) plays a key role in health, being essential for the protection against reactive oxygen species (ROS). Nevertheless, excessive oxidative stress can induce RPE dysfunction, promoting visual loss. Our aim is to clarify possible implication of CYP2E1 ethanol (EtOH)-induced alterations. Despite increase levels ROS, measured by fluorescence probes, cells exposed lowest EtOH concentrations were able maintain cell survival, Cell Proliferation Kit II (XTT)....
Choroidal dystrophies comprise a group of chorioretinal degenerations. However, the different findings observed among these patients make it difficult to establish correct clinical diagnosis. The objective this study was characterize new by optical coherence tomography (OCT) and angiography (OCTA) in patients. Four family members with PRPH2 gene mutation (p.Arg195Leu) were included. OCT performed at macula, thickness outer inner retina, total choroid measured. features vascular network...
The purinergic receptor P2X7 (P2X7R) is implicated in all neurodegenerative diseases of the central nervous system. It also involved retinal degeneration associated with glaucoma, age-related macular degeneration, and diabetic retinopathy, its overexpression retina evident these disorders. Retinitis pigmentosa a progressive degenerative disease that ultimately leads to blindness. Here, we investigated expression P2X7R during progression rd10 mouse model RP. As P2X4 widely co-expressed P2X7R,...
Abstract Central areolar choroidal dystrophy is an inherited disorder characterized by progressive choriocapillaris atrophy and retinal degeneration usually associated with mutations in the PRPH2 gene. We aimed to generate characterize a mouse model p.Arg195Leu mutation previously described patients. Heterozygous ( Prph2 WT/KI ) homozygous KI/KI mice were generated using CRISPR/Cas9 system introduce mutation. Retinal function was assessed electroretinography optomotor tests at 1, 3, 6, 9,...
Aims/Purpose: Central areolar choroidal dystrophy (CACD) is a rare hereditary disease that leads to progressive retinal and choriocapillaris degeneration vision loss. In Spain, there family suffering this disease, since they carry the mutation p.Arg195Leu in PRPH2 . Our team have generated mouse model of CACD carrying same as Spanish patients. aim was characterize progression retina structurally morphologically new murine model. Methods: We studied C57BL/6J‐Prph2 em1Sal by...