A5079281452- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Extracellular vesicles in disease
- interferon and immune responses
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- Eicosanoids and Hypertension Pharmacology
- E-Learning and Knowledge Management
- PARP inhibition in cancer therapy
- Cancer Mechanisms and Therapy
- Advanced biosensing and bioanalysis techniques
- Corneal Surgery and Treatments
- Educational Technology in Learning
- Saffron Plant Research Studies
- Trace Elements in Health
- melanin and skin pigmentation
- Mosquito-borne diseases and control
- Atherosclerosis and Cardiovascular Diseases
- Advanced Glycation End Products research
- Magnesium in Health and Disease
- Fatty Acid Research and Health
- Advanced Nanomaterials in Catalysis
- RNA regulation and disease
- Angiogenesis and VEGF in Cancer
University of Alicante
2022-2025
Université Paris-Saclay
2024
Institut des Neurosciences Paris-Saclay
2024
Centre National de la Recherche Scientifique
2024
Universidad de Salamanca
2023
Valencia Catholic University Saint Vincent Martyr
2015-2020
Universitat de València
2020
Different mechanisms have been suggested for cocaine neurotoxicity, including oxidative stress alterations. Nuclear factor kappa B (NF-κB), considered a sensor of and inflammation, is involved in drug toxicity addiction. NF-κB key mediator immune responses that induces microglial/macrophage activation under inflammatory processes neuronal injury/degeneration. Although cerebellum commonly associated to motor control, muscular tone, balance. Its relation with addiction getting relevance, being...
Abstract miR‐205‐5p is known to be involved in VEGF‐related angiogenesis and seems regulate associated cell signalling pathways, such as migration, proliferation apoptosis. Therefore, several studies have focused on the potential role of an anti‐angiogenic factor. Vascular observed diabetic retinopathy ‘wet’ form age‐related macular degeneration. Today, most common treatments against these eye‐related diseases are anti‐VEGF therapies. In addition, both AMD DR typically with oxidative stress;...
ARPE-19 retinal pigment epithelial cells cultured in a medium containing 35 mM D-glucose led to an augmented ROS formation and release of vascular endothelial factor (VEGF)-containing exosomes compared 5 (standard medium). Exposing these the melanocortin receptor agonist (MCR5) PG-901 (10−10M), for 9 d reduced generation, number released their VEGF content. In contrast, incubating with MCR1 BMS-470539 (10−5 M) or mixed MCR3/4 MTII (0.30 nmol) did not produce any significant decrease levels....
Abstract Retinitis Pigmentosa is a group of inherited neurodegenerative diseases that result in selective cell death photoreceptors. In the developed world, RP regarded as main cause blindness among working age population. The precise mechanisms eventually leading to remain unknown and date no adequate treatment for available. Poly ADP ribose polymerase (PARP) over activity involved photoreceptor degeneration pharmacological inhibition or genetic knock-down PARP1 protect photoreceptors mice...
Unlike mammals, some nonmammalian species recruit Müller glia for retinal regeneration after injury. Identifying the underlying mechanisms may help to foresee regenerative medicine strategies. Using a
Purpose: Central Areolar Choroidal Dystrophy (CACD) is a monogenic hereditary retinal disorder characterized by progressive degeneration. In most cases, CACD presents an autosomal dominant inheritance pattern in which patients carry single mutation the peripherin (PRPRH2) gene. This study functional, immunohistochemical and transcriptomic analysis of retinas mouse model carrying p.Arg195Leu PRPH2 Methods: Neural from Prph2 WT/WT WT/KI mice were used at different ages. Electroretinogram (ERG)...
Abstract Age related macular degeneration (AMD) is a common retina-related disease leading to blindness. Little known on the origin of disease, but it well documented that oxidative stress generated in retinal pigment epithelium and choroid neovascularization are closely involved. The study circulating miRNAs opening new possibilities terms diagnosis therapeutics. can travel associated lipoproteins or inside small Extracellular Vesicles (sEVs). A number reports indicate significant...
Ischemia is the main cause of cell death in retinal diseases such as vascular occlusions, diabetic retinopathy, glaucoma, or retinopathy prematurity. Although excitotoxicity considered primary mechanism during an ischemic event, antagonists glutamatergic receptors have been unsuccessful clinical trials with patients suffering ischemia stroke. Our purpose was to analyze if transient receptor potential channel 7 (TRPM7) could contribute dysfunction pathologies associated ischemia. By using...
The retinal pigment epithelium (RPE) plays a key role in health, being essential for the protection against reactive oxygen species (ROS). Nevertheless, excessive oxidative stress can induce RPE dysfunction, promoting visual loss. Our aim is to clarify possible implication of CYP2E1 ethanol (EtOH)-induced alterations. Despite increase levels ROS, measured by fluorescence probes, cells exposed lowest EtOH concentrations were able maintain cell survival, Cell Proliferation Kit II (XTT)....
Abstract Central areolar choroidal dystrophy is an inherited disorder characterized by progressive choriocapillaris atrophy and retinal degeneration usually associated with mutations in the PRPH2 gene. We aimed to generate characterize a mouse model p.Arg195Leu mutation previously described patients. Heterozygous ( Prph2 WT/KI ) homozygous KI/KI mice were generated using CRISPR/Cas9 system introduce mutation. Retinal function was assessed electroretinography optomotor tests at 1, 3, 6, 9,...
Diabetic retinopathy is a leading cause of vision loss and has been correlated with increased oxidative stress. The aim the present study was to evaluate protective properties docosahexaenoic acid (DHA), an omega-3 fatty acid, in human retinal pigment epithelial (RPE) cells exposed high glucose. Human RPE cell line (ARPE-19) cultured for 4 days followed by 5 exposure either normal (5.5 mM) or (45 D-glucose concentration absence presence DHA (100 µM). Reduced form glutathione (GSH), total...
Abstract Purpose Central areolar choroidal dystrophy is an inherited disorder characterized by progressive choriocapillaris atrophy and retinal degeneration, usually associated with mutations in the PRPH2 gene. We aimed to generate characterize a mouse model p.Arg195Leu mutation previously described patients. Methods Heterozygous ( Prph2 WT/KI ) homozygous KI/KI mice have been generated using CRISPR/Cas9 system introduce Arg195Leu mutation. Retinal function was assessed electroretinography...
Abstract Purpose: Parkinson's disease (PD) patients suffer from visual deficiencies in the early stages of disease. One these symptoms is impairment motion perception. In that sense, we wanted to determine if starburst amacrine cells (the main cell type involved perception) are degenerated PD. Also, sought evaluate this possible degeneration related dopaminergic system. Methods: Post‐mortem human eyes control and PD donors were used study. Immunohistochemistry confocal microscopy whole‐mount...