A5043967157- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Lipoproteins and Cardiovascular Health
- Cancer-related molecular mechanisms research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Peripheral Artery Disease Management
- Angiogenesis and VEGF in Cancer
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Immune Response and Inflammation
- Acute Myeloid Leukemia Research
- Circular RNAs in diseases
- Obesity, Physical Activity, Diet
- Cell Adhesion Molecules Research
- Pharmacology and Obesity Treatment
- MicroRNA in disease regulation
- Protein Interaction Studies and Fluorescence Analysis
- Kruppel-like factors research
- Lipid metabolism and disorders
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Lymphatic System and Diseases
- Blood Pressure and Hypertension Studies
- Cardiovascular Health and Disease Prevention
- Congenital heart defects research
- Platelet Disorders and Treatments
Brigham and Women's Hospital
2016-2025
Harvard University
2017-2025
Fundação Universitária de Cardiologia
2016-2019
Center for Systems Biology
2017
Nova Southeastern University
2017
Massachusetts General Hospital
2017
Superficial erosion currently causes up to a third of acute coronary syndromes; yet, we lack understanding its mechanisms. Thrombi because superficial intimal characteristically complicate matrix-rich atheromata in regions flow perturbation.This study tested vivo the involvement disturbed and neutrophils, hyaluronan, Toll-like receptor 2 ligation injury, process implicated erosion.In mouse carotid arteries with established lesions tailored resemble substrate human eroded plaques,...
Abstract Aims Recent evidence suggests that ‘vulnerable plaques’, which have received intense attention as underlying mechanism of acute coronary syndromes over the decades, actually rarely rupture and cause clinical events. Superficial plaque erosion has emerged a growing residual thrombotic complications atherosclerosis in an era increased preventive measures including lipid lowering, antihypertensive therapy, smoking cessation. The mechanisms remain poorly understood, we currently lack...
Objective: Vascular smooth muscle cell (VSMC) plasticity plays a critical role in the development of atherosclerosis. Long noncoding RNAs (lncRNAs) are emerging as important regulators vessel wall and impact cellular function through diverse interactors. However, lncRNAs regulating VSMCs atherosclerosis remains unclear. Approach Results: We identified VSMC-enriched lncRNA cardiac mesoderm enhancer-associated RNA (CARMN) that is dynamically regulated with progression In both mouse human...
Diabetes-associated atherosclerosis involves excessive immune cell recruitment and plaque formation. However, the mechanisms remain poorly understood. Transcriptomic analysis of aortic intima in Ldlr−/− mice on a high-fat, high-sucrose-containing (HFSC) diet identifies macrophage-enriched nuclear long noncoding RNA (lncRNA), MERRICAL (macrophage-enriched lncRNA regulates inflammation, chemotaxis, atherosclerosis). expression increases by 249% intimal lesions during progression. lncRNA-mRNA...
This study aimed to evaluate the effect of intake yerba mate (YM) and green tea (GT) on serum levels leptin paraoxonase-1 (PON-1), compared control. Controlled, randomized clinical trial (RCT) that evaluated 142 men women affected by overweight or obesity aged 35–60 years, untreated dyslipidemia no history coronary artery disease. Participants were ingest 1000 mL GT, YM apple (AT, control group) daily, during eight weeks. Serum PON-1 analyzed ELISA immunoassay at beginning (baseline) after...
Patients with peripheral artery disease (PAD) and diabetes have the highest risk of critical limb ischemia (CLI) amputation, yet underlying mechanisms remain incompletely understood. MicroRNA (miRNA) sequencing plasma from diabetic patients or without CLI was compared to mice acute subacute identify conserved miRNAs. miRNA-KO on high-fat diet were generated explore impact CLI. Comparison dysregulated miRNAs individuals PAD revealed miR-181 family members. High-fat–fed, Mir181a2b2-KO had...
Abstract Superficial plaque erosion, distinct from rupture, represents a cause of residual thrombotic complications in atherosclerosis. Being able to detect mechanism that now accounts for up 30% acute coronary syndromes, can lead targeted therapies, and perhaps avoid the need urgent invasive interventions required rupture cases, thus greatly affecting clinical management. Nanoparticles (NPs) exposed biological fluids acquire layer proteins on their surface, protein corona (PC). Variations...
In an attempt to increase the therapeutic potential for myocardial regeneration, there is a quest new cell sources and types therapy protocols. The pathophysiology of heart diseases may affect cellular characteristics results.To study proliferative differentiation mesenchymal stem cells (MSC), isolated from bone marrow (BM) sternum, we made comparative analysis between samples patients with ischemic (IHD) or non-ischemic valvular (VHD) diseases. We included IHD (n = 42) VHD 20), average age...
Background: Clonal hematopoiesis of indeterminate potential (CHIP) can contribute to cardiovascular risk. The Interleukin-6 receptor (IL6R) polymorphism (D358A) modifies the risk coronary artery diseases among CHIP carriers in human cohorts. However, effects IL6R D358A on IL-6 pathways are discordant with canonical expectation, that classical signaling pathway is anti-inflammatory while trans-signaling pro-inflammatory. Hypothesis: DNMT3A, most frequent driver gene for CHIP, changes...
Background: Clonal hematopoiesis of indeterminate potential (CHIP) refers to somatic mutations in hematopoietic cells with a minimum allele fraction ≥ 2% without hematological disease. CHIP depends on age and increases the risk coronary artery disease (CAD). In their 70s women’s prevalence cardiovascular catches up that men. Canakinumab Antiinflammatory Thrombosis Outcome Study (CANTOS) neutralizing interleukin-1β (IL-1β) chronic CAD, subjects TET2 showed greater reduction events. However,...
Introduction: In the current era of intense LDL lowering, superficial erosion may be on rise as a cause ACS (25-30%). Experimental data human atheromata support role for neutrophils and formation neutrophil extracellular traps (NETs) in pathogenesis subsequent thrombosis. The common mutation Janus kinase 2 ( Jak2 V617F ) borne by clones leukocytes derived from bone marrow stem cells sensitizes to undergo NETosis linked NET increased associates with accelerated atherosclerosis venous...
Introduction: Clonal hematopoiesis of indeterminate potential (CHIP) describes somatic mutations bone marrow derived blood cells in the absence overt hematological disease. CHIP correlates with aging and atherosclerotic cardiovascular diseases humans. Tet2 mutations, common CHIP, augment atherosclerosis. -deficient macrophages over-express Interleukin-(IL)1β mRNA response to LDL. IL-1β neutralization reduces recurrent events patients post myocardial infarction residual inflammatory risk.We...
Introduction: Diabetes-associated atherosclerosis is characterized by excessive vascular wall inflammation, including impaired lipid deposition, increased immune cell infiltration and plaque formation in the vessel wall. Macrophages are instrumental for lesion formation, however mechanisms underlying their functional capacity remain poorly understood diabetes. Herein, we identify a unique role of macrophage-enriched lncRNA MERRICAL (Macrophage Enriched Regulates Inflammation, Chemotaxis,...