The aim of the present study was to evaluate effects heat stress (HS) and methionine supplementation on markers gene expression levels uncoupling proteins ( UCP ), betaine–homocysteine methyltransferase BHMT cystathionine β-synthase CBS glutathione synthetase GSS ) peroxidase 7 GPx7 ). Broilers from 1 21 d 22 42 age were divided into three treatment groups related supplementation: without (MD); recommended level (DL1); excess (DL2). broilers either kept at a comfortable thermal temperature...

The objective of this study was to evaluate the effect heat stress and methionine supplementation on gene expression insulin-like growth factor I (IGF-I), hormone receptor (GHR), phosphatidylinositol 3-kinase, regulatory 1 (PI3KR1) in liver, as well atrogin cathepsin L2 (CTSL2) genes breast muscle broilers. Broilers from 1–21 22–42 days age were divided into three treatments related follows: without (MD), recommended level (DL1), excess (DL2). animals either maintained at a thermal comfort...

The aims of the present study were to evaluate possible effects heat stress (HS) on H2O2 production and whether methionine supplementation (MS) could mitigate deleterious cell metabolism redox state induced by oxidative stress. Meat quails (Coturnix coturnix coturnix) fed a diet that either met nutritional demands for or did not meet this demand (methionine deficient [MD] diet) 7 d. animals kept at thermal comfort temperature (25°C) exposed HS (38°C 24 h, starting sixth day). Heat decreased...

We aimed to evaluate the effects of acute heat stress (HS) and age on redox state in broilers aged 21 42 days. evaluated expression genes related antioxidant capacity, production hydrogen peroxide (H2O2), activity enzymes liver, as well oxidative markers liver plasma. The experiment had a completely randomized factorial design with two thermal environments (thermoneutral HS, 38°C for 24 h) ages (21 days). Twenty-one-day-old animals exposed HS showed highest thioredoxin reductase 1 (TrxR1)...

The aim of this study was to examine whether (1) severe changes in salinity produced increased stress, and (2) vitamin C supplementation might reduce the observed damage Nile tilapia. parameters measured included condition factor, survival rate, gene expression catalase (CAT), heat shock protein 70 (HSP70), glutathione reductase (GSR), synthase (GSS), peroxidase (GPx). investigation conducted with 160 tilapia divided into four treatment groups: freshwater; 7 or 21 parts per thousand (‰)...

This study evaluated the effects of Eimeria spp. challenge and dietary supplementation with free methionine or dipeptide on animal performance; expression genes associated immune system, antioxidant amino acid transport in jejunum; redox status jejunum broiler chickens.A randomized, 2 × 3 factorial design was used, which first factor (Eimeria-challenged, EC, unchallenged, UC, broilers) second (non-supplemented, NS; dl-methionine, dl-Met; dipeptide, dl-methionyl-dl-methionine, dl-MMet). At 14...

This study aimed to develop extruded snacks including flour obtained from Nile tilápia carcasses, and then evaluate the chemical composition, colorimetry, thiobarbituric acid reactive substances, microbiology. There was a significant increase in levels of crude protein, ether extract, ash, minerals, as well reduction carbohydrates more included snacks. Increased flavored caused brightness Oxidative rancidity not detected The inclusion improved nutritional value without changing sensory...

Genetically improved farmed tilapia (GIFT) is a strain of Nile ( Oreochromis niloticus ) developed for production and commercial parameters. Skin color, one the characteristics distinguishing varieties, an important phenotypic trait associated with qualitative productive performance. This study aimed to assess fillet skin in GIFT black red phenotypes. For this, 24 from same spawning stock were divided into two groups based on namely variety (dark skin) (reddish skin). There no significant...

This study examined the effects of dietary levels methionine on lipid and intestinal metabolism in Japanese quail hens their progeny. The experiment was conducted according to a 3 × factorial design, with three maternal progeny diets, as follows: low-methionine (LMET), recommended (MET), high-methionine (HMET). Methionine supplementation improved reproductive performance during laying (p < 0.05). Intestinal morphometry revealed that MET HMET diets increased duodenal villus width crypt depth...

A avicultura brasileira tem passado por mudanças significativas para atender às demandas do mercado, especialmente nas áreas de melhoramento animal, nutrição e sistemas criação. Este trabalho como objetivo avaliar a importância cálcio fósforo no desempenho zootécnico na qualidade da carne frangos corte. animal desempenha um papel central alcance potencial zootécnico, com destaque os minerais (Ca) (P), que são essenciais metabolismo energético, desenvolvimento ósseo funções fisiológicas. O é...

A produção de ovos é muito importante, pois garante uma fonte acessível proteína animal para a população. nutrição das poedeiras comerciais um fator determinante o desempenho produtivo, qualidade dos e bem-estar aves. formulação dietas deve garantir suprimento adequado energia, proteína, minerais vitaminas, ajustando-se às necessidades nutricionais cada fase produtiva. O balanço entre nutrientes essencial evitar deficiências ou excessos que possam comprometer eficiência alimentar saúde Esta...

The aim of the present study was to evaluate effect acute heat stress on production mitochondrial reactive oxygen species (ROS), gene expression avian uncoupling protein (avUCP) and glutathione peroxidase (GPX 7), activity enzyme GPX in liver meat quail. Two groups 15 quail (Coturnix coturnix japonica) that were 23 d age initially housed individually metallic cages. A period 7 provided for 2 bird adapt cages a thermoneutral environment at 25°C with 60% relative humidity. At 30 age, exposed...

This study aimed to evaluate liver and breast muscle insulin-like growth factor I (IGF-I) hormone receptor (GHR) gene expression between broilers fed different methionine levels sources.Broiler chicks were 22 42 days old, distributed in 5 treatments (control diet, DL1 -0.08% DL-methionine, DL2 -0.24% MHA-FA1 -0.11% hydroxy analoguefree acid, MHA-FA2 -0.33% analogue-free acid).The euthanized by cervical dislocation.RNA was extracted from muscle, followed cDNA synthesis amplification using...

Summary This study aimed to evaluate the effect of heat stress ( HS ) and selenium supplementation on markers stress, meat quality gene expression. For this, quails 42 days age were fed a diet that either met [0.33 mg/kg, nutritional demand for SS )] or did not meet [0.11 deficient SD demands during 7 evaluation. In addition, animals kept at thermal comfort temperature (25 °C) exposed (38 °C 24 h). Glutathione synthetase GSS ), glutathione reductase GSR uncoupling protein UCP expression...

We evaluated the effects of heat stress (HS) and methionine supplementation on biological markers expression genes for superoxide dismutase (SOD), thioredoxin (TRx), reductase 1 (TRxR1) sulfoxide A (MsrA) in broilers aged to 21 or 22 42 days. The were divided into two treatments, one with recommended level (MS, dl-2-hydroxy-4-methylthiobutanoic acid (dl-HMTBA)) without (MD). animals maintained at a temperature thermal comfort HS (38°C 24 h). Mortality was only observed 42-day-old exposed fed...

Calpastatin (CAST) is an endogenous calpain inhibitor and its main function to modulate the proteolytic action of enzymes responsible for post-mortem myofibril deterioration.The myostatin gene (GDF-8) acts as a negative regulator skeletal muscle growth.The expression these two genes, well their interaction, affects quality meat, especially tenderness phenotype.We evaluated genetic groups Santa Inês, ½ Dorper-Santa Inês White slaughtered with 2.0 mm, 2.5 mm 3.0 fat thickness, comparing levels...

Current study evaluated the effect of pre-slaughter stress on serum cortisol levels, pH, colorimetry, water-holding capacity (WHC) and gene expression ryanodine receptors (RyR1 RyR3) in Nile tilapia. A 3x4 factorial scheme experiment was conducted comprising three densities (100, 200, 400 kg/m³) with four transportation times (60, 120, 180, 240 minutes).Transportation alone reduced levels up to 180 minutes, followed by increased WHC mRNA expression, RyR1 RyR3 (200 kg/m³ density). No density...

The energy values and nutrient digestibility of shrimp waste meal (SWM), obtained from extractive fishing, were determined in 2 experiments with broiler chickens. In experiment 1, 180 chickens randomly distributed into 5 treatments 6 replicates birds each. corn-soybean basal diet (BD) 4 test diets consisted inclusion 50, 100, 150 or 200 g/kg SWM evaluated. 2, 240 (0, SWM) 10 Non-inclusion (0 g/kg) was a protein-free diet. metabolizable (ME) nitrogen-corrected (MEn) the ingredient (SWM) by...

1. A study was conducted to test the hypothesis that feed efficiency (FE) correlated with expression of genes from somatotropic axis and mitochondrial involved in energy production, environment which birds are exposed influenced such genes.2. Quails were divided into High-FE low-FE groups maintained a comfortable or heat stress (HS) (38°C for 24 h) evaluate changes insulin-like growth factor I (IGF-I), hormone receptor (GHR), adenine nucleotide translocator (ANT), uncoupling protein (UCP)...

Since cinnamon has vitamins and minerals in addition to antioxidants compounds its chemical composition studies have shown the potential of supplementation on some important characteristics performance birds. Thus, this study was conducted under hypothesis that inclusion laying quail diet could influence birds through expression genes related antioxidant activity lipid metabolism. To test hypothesis, 144 Japanese (Coturnix japonica) with an initial age 18 weeks average weight 133g were...

The enzyme 2′-5′ oligoadenylate synthase (OAS) is one of the key interferon-induced antiviral factors that act through inhibition viral replication. In chickens, there a single well-characterized OAS gene, synthase-like (OASL) has been shown to be upregulated after infection with various viruses. However, deeper understanding how chicken OASL acts against still necessary. this study, we tested hypothesis short interfering RNA (siRNA)–mediated knockdown would decrease host gene expression...

A strategy to mitigate the negative effects of stress on animals is enhance their ability beneficially respond stressful conditions. This study aimed assess whether prenatal ambient temperature influences response Japanese quail (Coturnix coturnix japonica) chicks environmental challenges during growth. The experiment was conducted in a 2 × factorial arrangement: two conditions for mothers (thermoneutral and heat by continuous exposure 32 °C) offspring intermittent 34 °C 6 h/day from 15 35...

This study aimed at evaluating the expression of mRNA avian uncoupling protein (avUCP), adenine nucleotide translocase (ANT), and cytochrome c oxidase subunit III (COX III), as well performance broilers fed diets containing two sources levels industrial methionine. Broilers between 22 42 days age were distributed into five treatments (basal diet, supplementation 0.08% dl-methionine, 0.24% 0.11% MHA-FA 0.33% MHA-FA). At end experimental period, birds sacrificed by neck dislocation their liver...

The aim of this work was to compare the tilapia (Oreochromis niloticus), pacu (Piaractus mesopotamicus) and tambaqui (Colossoma macroporum) skins on histology, composition, physico-chemical physico-mechanical features.Therefore, 10 kg each species were used.All have followed same methodology for tanning, all processed in tannery machine.Histological composition analysis conducted fresh skin, 20 leather used after tanning physical-