A5100435826- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Photosynthetic Processes and Mechanisms
- Peptidase Inhibition and Analysis
- Anodic Oxide Films and Nanostructures
- Cell Adhesion Molecules Research
- Cell Image Analysis Techniques
- Galectins and Cancer Biology
Harvard University
2010-2020
Boston Children's Hospital
2010-2020
Hokkaido University
2015
Within cells, the spatial compartmentalization of thousands distinct proteins serves a multitude diverse biochemical needs. Correlative super-resolution (SR) fluorescence and electron microscopy (EM) can elucidate protein relationships to global ultrastructure, but has suffered from tradeoffs structure preservation, retention, resolution, field view. We developed platform for three-dimensional cryogenic SR focused ion beam-milled block-face EM across entire vitreously frozen cells. The...
Abstract Living cells function through the spatial compartmentalization of thousands distinct proteins serving a multitude diverse biochemical needs. Correlative super-resolution (SR) fluorescence and electron microscopy (EM) has emerged as pathway to directly view nanoscale protein relationships underlying global ultrastructure, but traditionally suffered from tradeoffs structure preservation, retention, resolution, field view. We developed platform for three-dimensional correlative...