A5044632393- Cancer Genomics and Diagnostics
- Synthesis and Catalytic Reactions
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Chemical Synthesis and Analysis
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Bacterial Identification and Susceptibility Testing
- Click Chemistry and Applications
- Microbial infections and disease research
- Molecular Biology Techniques and Applications
- Pharmacological Receptor Mechanisms and Effects
Weill Cornell Medicine
2019-2020
Cornell University
2019-2020
β-Tryptase, a homotetrameric serine protease, has four identical active sites facing central pore, presenting an optimized setting for the rational design of bivalent inhibitors that bridge two adjacent sites. Using diol, hydroxymethyl phenols or benzoyl methyl hydroxamates, and boronic acid chemistries to reversibly join [3-(1-acylpiperidin-4-yl)phenyl]methanamine core ligands, we have successfully produced series self-assembling heterodimeric inhibitors. These tryptase demonstrate superior...
We have developed a novel high-throughput PCR-ligase detection reaction-capillary electrophoresis (PCR-LDR-CE) assay for the multiplexed identification of 20 blood-borne pathogens (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, faecium, Listeria monocytogenes, Streptococcus pneumoniae, pyogenes, agalactiae, Escherichia coli, Klebsiella Haemophilus influenzae, Pseudomonas aeruginosa, Acinetobacter baumannii, Neisseria meningitidis, Bacteroides...
Abstract Background Interrogation of site-specific CpG methylation in circulating tumor DNAs (ctDNAs) has been employed a number studies for early detection breast cancer (BrCa). In many these studies, the markers were identified based on known biology BrCa progression, and interrogated using methyl-specific PCR (MSP), technique involving bisulfite conversion, PCR, qPCR. Methods this report, we are demonstrating development novel assay (Multiplex Bisulfite PCR-LDR-qPCR) which can potentially...
Detection of low-abundance mutations in cell-free DNA is being used to identify early cancer and recurrence. Here, we report a new PCR-LDR-qPCR assay capable detecting point at single-molecule resolution the presence an excess wild-type DNA. Major features include selective amplification detection mutant employing multiple nested primer-binding regions as well sequence blocking oligonucleotides, prevention carryover contamination, spatial sample dilution, same position. Our method was tested...