A5044257332- Metabolism and Genetic Disorders
- Amino Acid Enzymes and Metabolism
- Mitochondrial Function and Pathology
- Drug Transport and Resistance Mechanisms
- Infectious Encephalopathies and Encephalitis
- Epigenetics and DNA Methylation
- Enzyme Structure and Function
- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Folate and B Vitamins Research
- Peroxisome Proliferator-Activated Receptors
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Chemical Synthesis and Analysis
- Metabolomics and Mass Spectrometry Studies
- Lanthanide and Transition Metal Complexes
- Molecular Sensors and Ion Detection
- Pharmacological Effects and Toxicity Studies
- Biochemical and Molecular Research
- DNA and Nucleic Acid Chemistry
- Heavy Metal Exposure and Toxicity
- RNA modifications and cancer
- Trace Elements in Health
- Hemoglobin structure and function
- Vitamin C and Antioxidants Research
University of Calabria
2016-2025
Toronto Metropolitan University
2015
University of Bari Aldo Moro
2010-2013
The large Amino Acid Transporter 1 (LAT1) is an interesting target in drug discovery since this transporter overexpressed several human cancers. Furthermore, due to its location the blood-brain barrier (BBB), LAT1 for delivering pro-drugs brain. In work, we focused on defining transport cycle of using silico approach. So far, studies interaction with substrates and inhibitors have not considered that must undergo at least four different conformations complete cycle. We built outward-open...
Inflammation is a physiological condition characterized by complex interplay between different cells handled metabolites and specific inflammatory-related molecules. In some pathological situations, inflammation persists underlying worsening the state. Over years, two membrane transporters namely OCTN1 (SLC22A4) OCTN2 (SLC22A5) have been shown to play roles in inflammation. These form OCTN subfamily within larger SLC22 family. The link these proteins has proposed based on their chronic...
Riboflavin, the FMN and FAD precursor, is a crucial vitamin in cell metabolism. Its adsorption tissue distribution are mediated by tree membrane transporters namely RFVT1-3. Mutations of their genes associated with Riboflavin Transporter Deficiency. Moreover, derangements level these have been found several human cancers. To obtain suitable experimental tool for studying function single proteins, testing effect pathological mutations validating predicted ligands as candidate drugs, we set up...
Abstract The type II glycoprotein CD98 (SLC3A2) is a membrane protein with pleiotropic roles in cells, ranging from modulation of inflammatory processes, host–pathogen interactions to association transporters the SLC7 family. recent resolution structure complex LAT1 showed that four Asn residues, N365, N381, N424, N506, harbour N-glycosylation moieties. Then, role on trafficking and stability was investigated by combining bioinformatics, site-directed mutagenesis cell biology approach....
The mitochondrial carnitine/acylcarnitine translocase has been identified, purified and reconstituted in liposomes 1990. Since that time it object of studies aimed to characterize its function define the molecular determinants translocation pathway. Thanks these tenacious map amino acids involved catalysis constructed roles critical residues pathway have elucidated. This possible through combination transport assay liposomes, site-directed mutagenesis, chemical labeling bioinformatics....
Abstract Exosomes are extracellular vesicles involved in cell-to-cell communication. Previous large scale proteomics revealed that they contain SLC proteins. However, no data on the function of exosomal SLCs is available, so far. An localized exosomes was here characterized for first time: carnitine transporter OCTN2 (SLC22A5). The protein detected by Western Blot analysis HEK293 exosomes. To investigate functional properties OCTN2, proteins extracted from were reconstituted into...
The effect of Hg2+ and CH3Hg+ on the mitochondrial carnitine/acylcarnitine transporter (CACT) has been studied recombinant protein CACT extracted from HeLa cells or Zebrafish reconstituted in proteoliposomes. Transport was abolished upon treatment proteoliposomes by CH3Hg+. Inhibition reversed SH reducing agent 1,4-dithioerythritol, GSH, N-acetylcysteine. IC50 for 90 nM 137 nM, respectively, were measured dose–response analyses. C-less mutant. Strong reduction inhibition both reagents...