A5101495302- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cellular Mechanics and Interactions
- Pluripotent Stem Cells Research
- Neuroscience and Neural Engineering
Stanford University
2022-2025
Abstract Direct ink writing is a 3D printing method that compatible with wide range of structural, elastomeric, electronic, and living materials, it continues to expand its uses into physics, engineering, biology laboratories. However, the large footprint, closed hardware software ecosystems, expense commercial systems often hamper widespread adoption. This work introduces compact, low‐cost, multimaterial, high‐throughput direct printer platform detailed assembly files instructions provided...
Combining the sustainable culture of billions human cells and bioprinting wholly cellular bioinks offers a pathway toward organ-scale tissue engineering. Traditional 2D methods are not inherently scalable due to cost, space, handling constraints. Here, suspension induced pluripotent stem cell-derived aggregates (hAs) is optimized using an automated 250 mL stirred tank bioreactor system. Cell yield, aggregate morphology, pluripotency marker expression maintained over three serial passages in...
Abstract Rheological measurements with in situ visualization can elucidate the microstructural origin of complex flow behaviors an ink. However, existing commercial rheometers suffer from high costs, need for dedicated facilities microfabrication, a lack design flexibility, and cabling that complicates operation sterile or enclosed environments. To address these limitations, low‐cost ($300) visual, in‐expensive wireless rheometer (VIEWR) using 3D‐printed off‐the‐shelf components is...
Direct ink writing is a 3D printing method that compatible with wide range of structural, elastomeric, electronic, and living materials, it continues to expand its uses into physics, engineering, biology laboratories. However, the large footprint, closed hardware software ecosystems, expense commercial systems often hamper widespread adoption. Here, we present compact, simple-to-build, low-cost, multimaterial, high-throughput direct printer platform detailed assembly files instructions...
Abstract Recent breakthroughs in biofabrication have enabled the development of engineered tissues for various organ systems, supporting applications drug testing and regenerative medicine. However, current approaches do not allow dynamic mechanical maturation tissue 3D. Although uniaxial mechanostimulation techniques shown promise generating anisotropic tissues, they fail to recapitulate biomechanics complex tissues. As a result, existing biofabricated lack ability replicate 3D alignment...