A5052068472- Electrochemical sensors and biosensors
- Electrocatalysts for Energy Conversion
- Electrochemical Analysis and Applications
- Genomics, phytochemicals, and oxidative stress
- Bioactive Compounds and Antitumor Agents
- Metalloenzymes and iron-sulfur proteins
- Enzyme Catalysis and Immobilization
- Photosynthetic Processes and Mechanisms
- CO2 Reduction Techniques and Catalysts
- Advanced biosensing and bioanalysis techniques
- Advanced battery technologies research
- Microbial Fuel Cells and Bioremediation
- Molecular Junctions and Nanostructures
- Coenzyme Q10 studies and effects
- Biochemical and Molecular Research
- Cancer, Hypoxia, and Metabolism
- Nanopore and Nanochannel Transport Studies
- Graphene research and applications
- Chalcogenide Semiconductor Thin Films
- Quantum Dots Synthesis And Properties
- Enzyme Structure and Function
- Porphyrin and Phthalocyanine Chemistry
- DNA Repair Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Adenosine and Purinergic Signaling
University of Manchester
2022-2025
University of Oxford
2016-2022
Science Oxford
2017-2019
Queen's University Belfast
2011-2019
A bio-hybrid material has been discovered, which offers a new direction for fast, specific enzyme-catalyzed organic synthesis.
In living cells, redox chains rely on nanoconfinement using tiny enclosures, such as the mitochondrial matrix or chloroplast stroma, to concentrate enzymes and limit distances that nicotinamide cofactors other metabolites must diffuse. a chemical analogue exploiting this principle, adenine dinucleotide phosphate (NADPH) NADP+ are cycled rapidly between ferredoxin-NADP+ reductase second enzyme-the pairs being juxtaposed within 5-100 nm scale pores of an indium tin oxide electrode. The...
This roadmap on Nanotechnology for Catalysis and Solar Energy Conversion focuses the application of nanotechnology in addressing current challenges energy conversion: 'high efficiency, stability, safety, potential low-cost/scalable manufacturing' to quote from contributed article by Nathan Lewis. solar-to-fuel conversion, solar water splitting, photovoltaics bio-catalysis. It includes dye-sensitized cells (DSSCs), perovskite cells, organic photovoltaics. Smart engineering colloidal quantum...
Abstract Multistep enzyme-catalyzed cascade reactions are highly efficient in nature due to the confinement and concentration of enzymes within nanocompartments. In this way, rates exceptionally high, loss intermediates minimised. Similarly, extended enzyme cascades trapped crowded nanoconfined environment a porous conducting metal oxide electrode material form basis powerful way study exploit myriad complex biocatalytic pathways. One confined enzymes, ferredoxin-NADP + reductase, serves as...
Protein film electrochemistry (PFE) has been used to study the assembly of complex 6Fe active site [FeFe]-hydrogenases (known as H-cluster) from its precursors-the [4Fe-4S] domain that is already coordinated within host, and 2Fe presented a synthetic water-soluble stabilized by an additional CO. Not only does PFE allow control redox states via electrode potential but also immobilized state enzyme facilitates extremely low concentrations complex. Results for two enzymes, CrHydA1 Chlamydomonas...
Reduction of CO2 and its direct entry into organic chemistry is achieved efficiently in a highly visible way using metal oxide electrode which two enzyme catalysts, one for electrochemically regenerating reduced nicotinamide adenine dinucleotide phosphate the other assimilating converting pyruvate (C3) to malate (C4), are entrapped within nanopores. The resulting reversible electrocatalysis exploited construct solar reduction/water-splitting device producing O2 C4 with high faradaic efficiency.
The ability to drive and observe rapid enzyme catalysis in both directions is an important natural consequence of immobilizing cascade components within electrode nanopores coupling reactions the fast, quasi-reversible NADP+/NADPH electrochemistry mediated by bound ferredoxin-NADP+ reductase. This approach has been exploited investigate control redox interconversions between a ketone secondary alcohol enantiomers catalyzed enantioselective dehydrogenase variants. An anticipated advantage...
Abstract In living cells, the overall rates of catalytic reaction chains (cascades) are massively enhanced by nanoconfinement enzymes in tiny enclosed volumes: presented such a way, interdependent catalysts highly concentrated, and distances (active site‐to‐active site) across which intermediates cofactors must diffuse, may be tiny. parallel technology exploiting this principle, enzyme cascades powered, amplified, monitored real time as they work concert, being nanoconfined within pores an...
The importance of energized nanoconfinement for facilitating the study and execution enzyme cascades that feature multiple exchangeable cofactors is demonstrated by experiments with carboxylic acid reductase (CAR), an requires both NADPH ATP during a single catalytic cycle. Conversion cinnamic to cinnamaldehyde package four enzymes loaded into trapped in random nanopores indium tin oxide (ITO) electrode driven monitored through simultaneous delivery electrical chemical energy. energy...
There are two common forms of NRH‐quinone oxidoreductase 2 (NQO2) in the human population resulting from SNP rs1143684. One has phenylalanine at position 47 (NQO2‐F47) and other leucine (NQO2‐L47). Using recombinant proteins, we show that these variants have similar steady state kinetic parameters, although NQO2‐L47 a slightly lower specificity constant. is less stable towards proteolytic digestion thermal denaturation than NQO2‐F47. Both inhibited by resveratrol, but NQO2‐F47 shows negative...
A one-pot fuel cell for specific, enzyme-catalysed organic synthesis, with continuous monitoring of rate and reaction progress, combines an electrode catalysing rapid, reversible diffusion-controlled interconversion NADP+ NADPH a Pt 2H+/H2 interconversion. This Communication demonstrates its performance characteristics using the reductive amination 2-oxoglutarate as test system.
Significance The maturation of each hydrogenase class describes the process that converts an apo-hydrogenase into active holoenzyme involving a machinery maturases. For [FeFe]-hydrogenases, synthesis and insertion catalytic dinuclear [2Fe H ]-cofactor is executed by 3 maturases (HydE, HydF, HydG) have already been studied extensively. However, final steps occurring within (cofactor recognition integration) not elucidated yet. Herein, we postulate molecular mechanism, exemplifying how...
NAD(P)H quinone oxidoreductase-1 (NQO1) is a homodimeric protein that acts as detoxifying enzyme or chaperone protein. Dicourmarol interacts with NQO1 at the binding site and can both inhibit activity modulate interaction of other proteins. We show dicoumarol related compounds to generates negative cooperativity between monomers. This does not occur in presence reducing cofactor, NAD(P)H, alone. Alteration Gly150 (but Gly149 Gly174) abolished dicoumarol-induced cooperativity. Analysis...
Enantioselective conversion of alcohols to ketones using air as the oxidant is achieved with high rates and efficiency an indium tin oxide electrode in which alcohol dehydrogenase a NADPH recycling enzyme are confined within nanopores.
Living organisms are characterized by the ability to process energy (all release heat). Redox reactions play a central role in biology, from transduction (photosynthesis, respiratory chains) highly selective catalyzed transformations of complex molecules. Distance and scale important: electrons transfer on 1 nm scale, hydrogen nuclei between molecules 0.1 extended catalytic processes (cascades) operate most efficiently when different enzymes under nanoconfinement (10 nm–100 scale). Dynamic...
Human isocitrate dehydrogenase (IDH1) and its cancer-associated variant (IDH1 R132H) are rendered electroactive through coconfinement with a rapid NADP(H) recycling enzyme (ferredoxin-NADP+ reductase) in nanopores formed within an indium tin oxide electrode. Efficient coupling to localized enables IDH activity be energized, controlled, monitored real time, leading directly thermodynamic redox landscape for accumulation of the oncometabolite, 2-hydroxyglutarate, that would occur biological...