A5044824154- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Spinal Cord Injury Research
- Autophagy in Disease and Therapy
- Cardiac Ischemia and Reperfusion
- Pulmonary Hypertension Research and Treatments
- Immune Response and Inflammation
- Peroxisome Proliferator-Activated Receptors
- ATP Synthase and ATPases Research
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Advanced Glycation End Products research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Abdominal Trauma and Injuries
- Nausea and vomiting management
- Organ Transplantation Techniques and Outcomes
- Calpain Protease Function and Regulation
- Hip and Femur Fractures
- S100 Proteins and Annexins
- Aortic aneurysm repair treatments
- GDF15 and Related Biomarkers
China Medical University
2016-2024
First Hospital of China Medical University
2017-2024
Fujian Medical University
2021
Background: Liver cancer ranks the top four malignant type worldwide, which needs effective and safe treatment. Ferroptosis is a novel form of regulated cell death driven by iron-dependent lipid peroxidation has been regarded as promising therapeutic target for cancers. In this work, we aimed to study effects anesthetic ketamine on proliferation ferroptosis liver cancer. Methods: Cell viability were detected counting kit 8 (CCK-8), colony formation, 5-ethynyl-2′-deoxyuridine (EdU) assay. was...
Abstract Background Ischemia-reperfusion (IR) affects microRNA (miR) expression and causes substantial inflammation. Multiple roles of the tumor suppressor miR-129-5p in cerebral IR have recently been reported, but its functions spinal cord are unclear. Here, we investigated role after IR, particularly regulating high-mobility group box-1 (HMGB1) Toll-like receptor (TLR)-3 pathway. Methods Ischemia was induced via 5-min occlusion aortic arch. The relationship between HMGB1 elucidated RT-PCR,...
Toll-like receptor 4 (TLR4) and TLR4 interactor with leucine-rich repeats (TRIL) play a crucial role in the inflammatory response. This study investigated of long noncoding RNA taurine-upregulated gene 1 (lncRNA TUG1) TRIL/TLR4 signaling spinal cord ischemia reperfusion (IR) injury. IR injury was induced experimental rats; knockdown TUG1 TRIL by intrathecal injection siRNAs overexpression pcDNA3.3-TRIL. The results showed that mRNA levels were increased at 12 hours after IR; this accompanied...
Background Neuroinflammation and cellular apoptosis caused by spinal cord ischemia/reperfusion (I/R) injury result in neurological dysfunction. MicroRNAs (miRs) have crucial functions I/R pathogenesis according to previous evidences. Herein, whether miR-128-3p contributes regulating specificity protein 1 (SP1) was assessed. Methods A rat model of established occluding the aortic arch for 14 min. Then, miR-128-3p’s interaction with SP1 detected dual-luciferase reporter assays. Next, mimic...
Ischaemia reperfusion (IR) induces multiple pathophysiological changes. In addition to its classical role in regulating tumourigenesis, the feedback loop formed by p53 and driven target p53-upregulated modulator of apoptosis (PUMA) was recently demonstrated be common node tightly controlling various cellular responses during myocardial IR. However, roles p53-PUMA spinal cord remain unclear. This study aimed elucidate interactions after IR, specifically investigating their regulation caspase...
Immune-inflammatory mechanisms contribute greatly to the complex process leading type A aortic dissection (TAAD). This study aims explore immune infiltration and key immune-related genes in acute TAAD.ImmuCellAI algorithm was applied analyze patterns of TAAD samples normal vessel GSE153434 dataset. Differentially expressed (DEGs) were screened. Immune-related obtained from overlapping DEGs ImmPort database. The hub based on protein-protein interaction (PPI) network. validated GSE52093...
miR-101a-3p is expressed in a variety of organs and tissues plays regulatory role many diseases, but its spinal cord ischemia/reperfusion injury remains unclear. In this study, we established rat model by clamping the aortic arch for 14 minutes followed reperfusion 24 hours. Results showed that expression L4-L6 was greatly decreased, whereas MYCN increased. Dual-luciferase reporter assay results targeted MYCN. immunoreactivity, which primarily colocalized with neurons tissue, increased after...
Spinal cord ischemia/reperfusion injury (SCII) is a severe complication driven by apoptosis and neuroinflammation. An increase in the expression of c-Fos, member AP-1 family, known as neuronal activation marker SCII. The family composed Jun, Fos, associated with regulation cytokines apoptosis. Fra-1 Fos however, contribution to SCII still unclear. In our study, was highly upregulated especially neurons microglia promoted changing Bax/Bcl-2 after Furthermore, we found that directly regulated...
Lung cancer, recognized globally as a leading cause of malignancy-associated morbidity and mortality, is marked by its high prevalence lethality, garnering extensive attention within the medical community. Mitophagy critical cellular process that plays crucial role in regulating metabolism ensuring quality control cells. Its relevance to lung cancer has garnered significant among researchers scientists. Mitophagy’s involvement encompasses initiation, progression, metastatic dissemination...
BACKGROUND:Intestinal ischemia/reperfusion (I/R) injury is a serious clinical complication. This study aimed to explore the hub genes and pathways of intestinal I/R injury. MATERIAL AND METHODS:GSE96733 from GEO website was extracted analyze differentially expressed (DEGs) injured sham-operated mice at 3 h 6 after surgery. The DAVID STRING databases were used construct functional enrichment analyses DEGs protein–protein interaction (PPI) network. In Cytoscape software, cytoHubba identify...
Spinal cord ischemia-reperfusion (I/R) injury is an unresolved complication and its mechanisms are still not completely understood. Here, we studied the neuroprotective effects of dexmedetomidine (DEX) postconditioning against spinal I/R in rats explored possible mechanisms.In study, were randomly divided into five groups: sham group, DEX0.5 DEX2.5 DEX5 group. was induced experimental rats; 0.5 μg/kg, 2.5 5 μg/kg DEX intravenously injected upon reperfusion respectively. Neurological...
Spinal cord ischemia/reperfusion injury (SCII) is a catastrophic complication involved with cardiovascular, spine, and thoracic surgeries can lead to paraplegia. Nevertheless, the molecular mechanism of SCII remain ill-defined.Expression profiling (GSE138966) data were obtained from GEO database. Then, differentially expressed (DE) lncRNAs DEmRNAs screened out p < 0.05, | fold change| > 1.5. Aberrant miRNAs expression in was PubMed. Functional enrichment analysis overlapping between...
Spinal cord ischemia/reperfusion injury (SCII) is one of the most serious spinal complications that stem from varied spine injuries or thoracoabdominal aortic surgery. Nevertheless, molecular mechanisms underlying SCII remain unclear.Male Sprague-Dawley (SD) rats were randomly divided into 5 groups sham, 24 h, 72 sevoflurane preconditioning h (SCII h+sevo), and h+sevo) group. We then analyzed expression differentially expressed micro RNAs (DEmiRNAs) in these their target genes. Functional...
Spinal cord ischemia–reperfusion injury (SCII) is still a serious problem, and the mechanism not fully elaborated. In rat SCII model, qRT-PCR was applied to explore altered expression of miR-9 (miR-9a-5p) after SCII. The biological function its potential target genes based on bioinformatics analysis experiment validation in were explored next. Before surgical procedure SCII, mimic inhibitor intrathecally infused. improved neurological function. addition, reduced blood-spinal barrier (BSCB)...
Spinal cord ischemia-reperfusion injury (SCII) is a pathological process with severe complications such as paraplegia and paralysis. Aberrant miRNA expression involved in the development of SCII. Differences experimenters, filtering conditions, control selection, sequencing platform may lead to different results. This study systematically analyzes available SCII data explore key differently expressed miRNAs (DEmiRNAs) underlying molecular mechanism A systematic bioinformatics analysis was...