The spatial-temporal dynamics of an in vitro radicular system Typha domingensis for the development rhizofiltration technologies, with potential use as a phytotreatment eutrophicated water, were studied first time roots seedlings and rhizotron systems. effect indole-3-acetic acid (AIA) combination kinetin (CIN) or 6-benzylaminopurine (BAP) on cultivated light dark three systems rhizotrophic regime screening dynamic rhizogenic lines, by weekly allometric measurements length number roots,...

3-Hydroxy-4,5-dimethyl-2(5H)-furanone (sotolone) and 3-amino-4,5-dimethyl-2(5H)-furanone, the postulated precursor of sotolone, were detected in hairy root cultures Trigonella foenum-graecum (fenugreek) by GC-MS. The both conical flasks airlift with mesh bioreactors achieved from hypocotyl seedling infection Agrobacterium rhizogenes. In flasks, mathematical relationship between growth conductivity was established afterward used to evaluate biomass evolution bioreactor due difficulty...

Due to their antioxidant properties, polyphenolic compounds are considered beneficial for human health. In this work, we investigated the polyphenol profile and activity of edible tejocote ( Crataegus pubescens ) fruit extracts by micellar electrokinetic chromatography (MEKC) HPLC/UV. The major phenolic in pulp were (+)-catechin (9.17 ± 0.20 mg/100 mg dry fruit), (−)-epicatechin (4.32 0.11 chlorogenic acid (5.60 0.24 fruit). total content was 168.6 0.9 gallic equivalent/g fruit;...

Oil spills are the most common source of environmental damage in areas with oil extraction activities.Bioaugmentation is a bioremediation technology that involves increasing microbiotas can remove contaminant compounds from soils or water.This be achieved by supplying exogenous microorganisms abundance native able to contaminants.As biosurfactants enhance solubility and bioavailability hydrophobic compounds, addition bacteria produce may improve rates hydrocarbons biodegradation at spill...

To date, there is an urgent need for implementing practical strategies to reduce CH 4 emissions from ruminants. Lovastatin (Lv) a specific inhibitor of methanogenic archaea. Due the high cost pure Lv, solid-state fermentation might be economical bioprocess produce Lv and facilitate its use in ruminant nutrition. The goal this work was assess effects supplementing fermented oat straw as lovastatin carrier (FOS) high-grain ration on vitro inhibition rumen microbiota beef cattle. experimental...

Expression systems based on plant cells, tissue, and organ cultures have been investigated as an alternative for production of human therapeutic proteins in bioreactors. In this work, hairy root Brassica oleracea var. italica (broccoli) were established airlift with mesh bioreactor to produce isoform 1 the growth hormone (hGH1) a model protein. The hGH1 cDNA was cloned into pCAMBIA1105.1 binary vector induce roots hypocotyls broccoli plantlets via Agrobacterium rhizogenes. Most infected...

The effect of recalcitrant hydrocarbons on the fatty acid profile from leaf, basal corm, and roots Cyperus laxus plants cultivated in greenhouse phytoremediation systems soils aged oil spill-impacted sites containing 16 to 340 g/Kg total (THC) was assessed investigate if this is a C18:3 species hydrocarbon removal during process has relationship with plant. specific each vegetative organ strongly affected by level impacted sites. Leaf extracts uncontaminated soil produced palmitic (C16),...

Several nitrogen fixing consortia (NFC) were isolated from kerosene contaminated soil, where Phaseolus vulgaris plants being cultivated. The capability of these for hydrocarbons removal was investigated and demonstrated. NFC cultivated under aerobic conditions, as sole carbon source, effected a maximum 75% reduction the total hydrocarbons. Also, experiments conducted to evaluate their atmospheric capability, all showed nitrogenase activity: 4 183 nmol N2/3E09 bacteria.day. To our knowledge,...

The effect of mixing low amounts orange peel (Citrus reticulata) with a soil contaminated hydrocarbons (58,000 mg kg(-1) soil) for promoting the remediation in solid culture was studied. experimental design established at soil/orange ratios 100:0, 98:2, 96:4, 94:6 and 92:8, 30% humidity C:N:P ratio 100:10:1, 15, 60 90 days, respectively. total petroleum (TPHs) decreased significantly (69%) after 15 days treatment to 92:8. Furthermore, this bacterial counts increased from 17 20 ln CFU (2.6 x...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCapsaicin recovery from a cell culture brothJ. Carlos Tapia, Rafael Garcia, Eleazar M. Escamilla, Graciano Calva, and J. Antonio RochaCite this: Ind. Eng. Chem. Res. 1993, 32, 10, 2242–2246Publication Date (Print):October 1, 1993Publication History Published online1 May 2002Published inissue 1 October 1993https://pubs.acs.org/doi/10.1021/ie00022a007https://doi.org/10.1021/ie00022a007research-articleACS PublicationsRequest reuse permissionsArticle...

A protocol for the induction of hairy roots on somatic embryos rhizoclones from Typha domingensis seedlings grown in hydroponic rhizotron systems was established first time. Rhizogenesis induced through agrotransformation oblong and scutellar states development using K599, LBA9402, A4 strains Agrobacterium rhizogenes. The transfection to performed by cocultivation a Murashige Skoog mineral medium at 50% strength (MS0.5), dark, 28 ± 2 °C 72 h. In contrast nontransformed that did not exhibit...

HPLC-UV, 1H NMR, 13C and 1H−1H COSY analyses revealed that exogenous capsaicin was specifically converted into 5,5'-dicapsaicin by both cell suspension cultures of Capsicum annuum var. (chili Jalapeño chigol) their soluble NaCl-extracted wall protein fractions under oxidative conditions. In found only in biomass capsaicin-fed cultures. This compound has not been detected before either fresh fruits or vitro Capsicum. The transformation different most the enzymatic activity located...

Antibiotic resistance occurs when the use of antibiotics to treat bacterial infections or select for transformed bacteria from in vitro cultures becomes ineffective.Although this phenomenon is primarily observed medicine, it also affects success scientific research bacterial-based genetic transformation experiments are performed.During agrotransformation plant cells, tissue, and organs, acquisition antibiotic by Agrobacterium spp.has been widely observed, making difficult bacteria.The...