A5047187913- Microfluidic and Capillary Electrophoresis Applications
- Cancer Cells and Metastasis
- Skin and Cellular Biology Research
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microtubule and mitosis dynamics
- Mass Spectrometry Techniques and Applications
- Terahertz technology and applications
- Analytical Chemistry and Chromatography
- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- Advanced Chemical Sensor Technologies
- Metabolomics and Mass Spectrometry Studies
- Neurogenesis and neuroplasticity mechanisms
- Receptor Mechanisms and Signaling
- Photonic and Optical Devices
- Nanopore and Nanochannel Transport Studies
- Spectroscopy and Laser Applications
- Cancer Research and Treatments
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Biosensors and Analytical Detection
- Molecular Biology Techniques and Applications
- X-ray Diffraction in Crystallography
- Plant Molecular Biology Research
Toho University Medical Center Sakura Hospital
2011-2025
Toho University
2011-2025
Kyushu University
2021-2025
National Cancer Research Institute
2024
RIKEN Center for Biosystems Dynamics Research
2018-2024
Osaka University
2010-2021
Ube Frontier University
2017-2020
Otsu Municipal Hospital
2019-2020
Chiba Institute of Technology
2020
Japan Science and Technology Agency
2014-2019
To investigate the physiological role of α1D-adrenergic receptor (α1D-AR) subtype, we created mice lacking α1D-AR (α1D–/–) by gene targeting and characterized their cardiovascular function. In α1D–/– mice, RT-PCR did not detect any transcript in tissue examined, there was no apparent upregulation other α1-AR subtypes. Radioligand binding studies showed that capacity aorta lost, while heart unaltered mice. Non-anesthetized maintained significantly lower basal systolic mean arterial blood...
We examined the utility of quantification α-synuclein (SNCA) in cerebrospinal fluid (CSF) to differentiate patients with Alzheimer disease (AD), dementia Lewy bodies (DLB), Parkinson (PD), and multiple system atrophy (MSA).Thirty-seven were divided into 4 age-matched sex-matched clinical groups: AD (n = 9), DLB 6), PD 11), MSA 11). Eleven subjects served as neurological controls. The total 48 included 27 men 21 women, aged 66.5 ± 11.4 years. performed a solid-phase sandwich enzyme-linked...
Recently, there has been increased attention on the analysis of circulating tumor cells ( CTC s), also known as liquid biopsy, owing to its potential benefits in cancer diagnosis and treatment. Circulating are released from primary lesions into blood stream eventually metastasize distant body organs. However, a major hurdle with is their natural scarcity. Existing methods lack sensitivity, specificity, or reproducibility required characterization detection. Here, we report untargeted...
Single cell metabolome analysis is essential for studying microscale life phenomena such as neuronal networks and tumor microenvironments. Capillary electrophoresis-mass spectrometry (CE-MS) one of the most sensitive technologies; however, its sensitivity still not enough single on general human cells HeLa. To address these issues, we first developed an efficient ionization emitter, named a "nanoCESI" that had thin-walled (∼10 μm) tapered (5-10 end. The thin conductive wall enabled...
To investigate the physiological role of α1D-adrenergic receptor (α1D-AR) subtype, we created mice lacking α1D-AR (α1D–/–) by gene targeting and characterized their cardiovascular function. In α1D–/– mice, RT-PCR did not detect any transcript in tissue examined, there was no apparent upregulation other α1-AR subtypes. Radioligand binding studies showed that capacity aorta lost, while heart unaltered mice. Non-anesthetized maintained significantly lower basal systolic mean arterial blood...
d-Amino acids (d-AAs) are endogenous molecules found throughout the metazoan, functions of which remain poorly understood. Measurements low abundance and heterogeneously distributed d-AAs in complex biological samples, such as cells multicellular structures central nervous system (CNS), require implementation sensitive selective analytical approaches. In order to measure d- l-forms aspartate glutamate, we developed applied a stacking chiral capillary electrophoresis (CE) with laser-induced...
The applicability of an online preconcentration technique, large-volume sample stacking with electroosmotic flow pump (LVSEP), to microchip zone electrophoresis (MCZE) for the analysis oligosaccharides was investigated. Since and separation proceeded continuously without polarity switching in LVSEP, a single "straight" channel could be employed. In MCZE oligosaccharides, adsorption onto surface should suppressed, so straight microchannel modified poly(vinyl alcohol) (PVA). So far, mechanism...
Dwarfism is a common type of mutation in many plant species. The pathways and factors regulating biosynthesis signaling several growth regulators have been clarified through analyses dwarf mutants rice, Arabidopsis, pea, maize. However, the genetic mechanisms controlling dwarfism are not well characterized, causal genes underlying most still uncovered. Here, we report dominant mutant, Twisted 1-1 (Tid1-1), showing twisted rice. Tid1-1 exhibit right helical leaves stem shortening roots. They...
Abstract Dementia with Lewy bodies (DLB) is the second most common neurodegenerative dementia after Alzheimer's disease (AD). Early differentiation of these disorders crucial for managing core symptoms; however, existing biomarkers remain insufficient. DLB shares motor and cognitive symptoms Parkinson’s (PD), both are classified as synucleinopathies due to abnormal α-synuclein aggregation. Although predominantly expressed in central nervous system, it also abundant erythrocytes. Recent...
Neurogenesis in the brain continues throughout life and is promoted by insults including ischemia. There no critical conclusion, however, about whether proliferated cells acquire neuronal function after Transient global ischemia was produced a four-vessel occlusion procedure rats (n = 54). To label proliferative cells, were administrated with single dose of 5-bromo-2'-deoxyuridine (BrdU) at 4, 6, 8, 10, 13, or 15 days Increases BrdU-positive detected hippocampal dentate gyrus 5, 7, 9...
A simple method for micro-casting with agarose gel was developed. Vacuum pressure in a degassed PDMS elastomer acted as driving force introducing solution into micro-channels. The repellency of well-guided cell adhesion area.
A combination of two online sample concentration techniques, large‐volume stacking with an electroosmotic flow (EOF) pump (LVSEP) and field‐amplified injection (FASI), was investigated in microchip electrophoresis (MCE) to achieve highly sensitive analysis. By applying reversed‐polarity voltages on a cross‐channel microchip, anionic analytes injected throughout microchannel were first concentrated the basis LVSEP, followed by electrokinetic from reservoir FASI mechanism. As well as voltage...
A combination of two online sample concentration techniques, large-volume stacking with an electroosmotic flow pump (LVSEP) and field-amplified injection (FASI), was investigated in CE to achieve highly sensitive oligosaccharide analysis. In LVSEP-FASI, analytes injected throughout the capillary were concentrated on basis LVSEP, followed by electrokinetic introduction from inlet vial FASI mechanism. After switching solution running buffer, then separated CZE. present LVSEP-FASI-CZE, pressure...
GPR52 is a Gs-coupled G protein-coupled receptor that predominantly expressed in the striatum and nucleus accumbens (NAc) was recently proposed as potential therapeutic target for schizophrenia. In current study, we investigated vitro vivo pharmacologic activities of novel agonist, 4-(3-(3-fluoro-5-(trifluoromethyl)benzyl)-5-methyl-1H-1,2,4-triazol-1-yl)-2-methylbenzamide (FTBMT). FTBMT functioned selective agonist vivo, demonstrated by activation Camp signaling striatal neurons. inhibited...
In our previous study, the combination of two on-line sample preconcentration techniques, large-volume stacking with an electroosmotic flow (EOF) pump (LVSEP) and transient isotachophoresis (tITP), in microchip electrophoresis (MCE) was developed, which named dual by (LDIS). LDIS apparently effective for improving sensitivity peak shape. LDIS, however, there a limit to improvement enhancement factor (SEF) since amount analytes be concentrated limited channel volume. To overcome this issue,...
Recently, microfluidic lab-on-a-CD (LabCD) has attracted attentions of researchers for its potential pumpless, compact, and chip-inclusive on-site bioassay. To control the fluids in LabCD, microvalves such as capillary, hydrophobic, siphon, sacrificial valves have been employed. However, no microvalve can regulate more than one channel. In a complicated bioassay with many sequential mixing, washing, wasting steps, thus, an intricate fluidic network microchannels, microvalves, reservoirs is...